Click image to see more details
Product Info Summary
| SKU: | PB9460 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Wnt1 Antibody Picoband®
SKU/Catalog Number
PB9460
PB0484 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Wnt1 Antibody Picoband® catalog # PB9460. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Wnt1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9460)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt1, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9460 is reactive to WNT1 in Human, Mouse, Rat
Observed Molecular Weight
45 kDa
Calculated molecular weight
41.0 kDa
Background of WNT1
Wingless-type MMIV integration site family, member 1 is a protein that in humans is encoded by the WNT1 gene. This gene is a member of the WNT gene family. The gene was assigned to human chromosome 12q13.12. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. The Wnt1 protein functions in the induction of the mesencephalon and cerebellum. However, the gene mutations might not have a significant role in Joubert syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9460 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human Jurkat whole cell, human CACO-2 whole cell, human Hela whole cell, human HepG2 whole cell, rat testis tissue, rat C6 whole cell, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
Click image to see more details
FBXO45 activates the Wnt/β-catenin signaling pathway. (A) KEGG enrichment analysis. (B) Pearson correlation analysis revealing the relationship between FBXO45 and β-catenin. (C) GSEA of the FBXO45 and Wnt/β-catenin signaling pathway. (D, E) Western blot analysis assessing the impact of FBXO45 knockdown on key proteins in the Wnt/β-Catenin pathway in A2780 and HEY cells. (F) Western blot analysis of FBXO45 and WNT1 expression in three normal ovarian tissues and three OV tissues, accompanied by statistical analysis (* p < 0.05; ** p < 0.01; *** p < 0.001).
Index in PubMed under a CC BY license. PMID: 40990020
Click image to see more details
Western blot analysis of Wnt1 using anti-Wnt1 antibody (PB9460).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt1 antigen affinity purified polyclonal antibody (PB9460) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Wnt1 at approximately 45 kDa. The expected band size for Wnt1 is at 41 kDa.
Specific Publications For Anti-Wnt1 Antibody Picoband® (PB9460)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Wnt1 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Wnt1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Wnt1 Antibody Picoband®
Question
Is this PB9460 anti-Wnt1 antibody reactive to the isotypes of WNT1?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The immunogen of PB9460 anti-Wnt1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt1 (45-74aa NLLTDSKSLQLVLEPSLQLLSRKQRRLIRQ), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-23
Question
We are currently using anti-Wnt1 antibody PB9460 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
The anti-Wnt1 antibody (PB9460) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-11
Question
I see that the anti-Wnt1 antibody PB9460 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-24
Question
See below the WB image, lot number and protocol we used for testis using anti-Wnt1 antibody PB9460. Please let me know if you require anything else.
S. Patel
Verified customer
Asked: 2018-04-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-11
Question
Would anti-Wnt1 antibody PB9460 work on dog WB with nucleus accumbens?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
Our lab technicians have not validated anti-Wnt1 antibody PB9460 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Wnt1 antibody PB9460 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog nucleus accumbens in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Wnt1 antibody PB9460. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-08
Question
I have a question about product PB9460, anti-Wnt1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Lewis
Verified customer
Asked: 2016-12-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9460 anti-Wnt1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-12-23
