Anti-Wnt1 Antibody Picoband™

Wnt-1 antibody

Boster Bio Anti-Wnt1 Antibody Picoband™ catalog # PB9460. Tested in WB applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: PB9460
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Customers Who Bought This Also Bought

Product Name

Anti-Wnt1 Antibody Picoband™

View all Wnt-1 Antibodies

SKU/Catalog Number

PB9460

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Wnt1 Antibody Picoband™ catalog # PB9460. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Wnt1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9460)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9460 is reactive to WNT1 in Human

Applications

PB9460 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

41 kDa

Calculated molecular weight

40.982kDa

Background of Wnt-1

Wingless-type MMIV integration site family, member 1 is a protein that in humans is encoded by the WNT1 gene. This gene is a member of the WNT gene family. The gene was assigned to human chromosome 12q13.12. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. The Wnt1 protein functions in the induction of the mesencephalon and cerebellum. However, the gene mutations might not have a significant role in Joubert syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For WNT1 (Source: Uniprot.org, NCBI)

Gene Name

WNT1

Full Name

Proto-oncogene Wnt-1

Weight

40.982kDa

Superfamily

Wnt family

Alternative Names

Int-1; INT1Proto-oncogene Int-1 homolog; proto-oncogene Wnt-1; wingless-type MMTV integration site family, member 1 (oncogene INT1); wingless-type MMTV integration site family, member 1; Wnt1; Wnt-1 WNT1 BMND16, INT1, OI15 Wnt family member 1 proto-oncogene Wnt-1|proto-oncogene Int-1 homolog|wingless-type MMTV integration site family member 1|wingless-type MMTV integration site family, member 1 (oncogene INT1)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on WNT1, check out the WNT1 Infographic

WNT1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for WNT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9460 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

MicroRNA-148a inhibits breast cancer migration and invasion by directly targeting WNT-1

Dong L, Duan Xc, Han Cx, Zhang H, Wu Y. Oncol Lett. 2015 Jan;9(1):81-85. Epub 2014 Oct 30. Suppression Of Wingless-Type Mmtv Integration Site Family, Member 1 Expression By Small Interfering Rna Inhibits U251 Glioma Cell Growth In Vitro.

Mi L, Li Y, Zhang Q, Zhao C, Peng Y, Yang G, Zheng X. Biochem Cell Biol. 2015 Feb;93(1):8-15. Doi: 10.1139/Bcb-2014-0079. Epub 2014 Sep 2. Microrna-139-5P Regulates C2C12 Cell Myogenesis Through Blocking Wnt/??-Catenin Signaling Pathway.

Have you used Anti-Wnt1 Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Wnt1 Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Anti-Wnt1 Antibody Picoband™

Question

Is this PB9460 anti-Wnt1 antibody reactive to the isotypes of WNT1?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

The immunogen of PB9460 anti-Wnt1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt1 (45-74aa NLLTDSKSLQLVLEPSLQLLSRKQRRLIRQ), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-23

Question

We are currently using anti-Wnt1 antibody PB9460 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-11

Answer

The anti-Wnt1 antibody (PB9460) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-11

Question

I see that the anti-Wnt1 antibody PB9460 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-24

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-24

Question

See below the WB image, lot number and protocol we used for testis using anti-Wnt1 antibody PB9460. Please let me know if you require anything else.

S. Patel

Verified customer

Asked: 2018-04-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-11

Question

Would anti-Wnt1 antibody PB9460 work on dog WB with nucleus accumbens?

Verified Customer

Verified customer

Asked: 2018-01-12

Answer

Our lab technicians have not validated anti-Wnt1 antibody PB9460 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Wnt1 antibody PB9460 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog nucleus accumbens in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-12

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Wnt1 antibody PB9460. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-11-08

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-08

Question

I have a question about product PB9460, anti-Wnt1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

E. Lewis

Verified customer

Asked: 2016-12-23

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9460 anti-Wnt1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-12-23

Order DetailsPrice
PB9460

100μg

$370
PB9460-10ug

10μg sample (liquid)

$99
PB9460-Biotin

100 μg Biotin conjugated

$570
PB9460-Cy3

100 μg Cy3 conjugated

$570
PB9460-Dylight488

100 μg Dylight488 conjugated

$570
PB9460-Dylight550

100 μg Dylight550 conjugated

$570
PB9460-Dylight594

100 μg Dylight594 conjugated

$570
PB9460-FITC

100 μg FITC conjugated

$570
PB9460-HRP

100 μg HRP conjugated

$570
PB9460-APC

100 μg APC conjugated

$670
PB9460-PE

100 μg PE conjugated

$670
PB9460-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
PB9460
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.