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Product Info Summary
| SKU: | M04574 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-FOLR2 Purified Monoclonal Antibody
SKU/Catalog Number
M04574
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FOLR2 Purified Monoclonal Antibody (Catalog# M04574). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-FOLR2 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04574)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
EM-35
Isotype
Mouse IgG1
Immunogen
BW5147alpha,beta- cells. The mouse monoclonal antibody EM-35 recognizes an extracellular epitope on FOLR2, a 30-40 kDa cell surface protein serving as a receptor for folic acid.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M04574 is reactive to FOLR2 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
29.3 kDa
Background of FOLR2
FOLR2 (folate receptor beta) is a cell surface protein that was originally thought to be specific for placenta, but it can be also expressed in other tissues, including hematopoietic cells. Its expression is increased in malignant tissues. FOLR2 may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. FOLR2 is a marker for macrophages generated in the presence of M-CSF (M2), including M2-like tumor-associated macrophages, which exert potent immunosuppressive functions within the tumor environment, but not GM-CSF (M1), and whose expression correlates with increased folate uptake ability.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04574 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: Non-reducing conditions.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Western blotting analysis of FOLR2 in THP1 cells (A) and FOLR2-transfected THP1 cells (B) using anti-FOLR2 (EM-35) purified.
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Separation of FORL2 transfected BW5147 cells stained using anti-FOLR2 (EM-35) purified antibody (concentration in sample 4 µg/ml, GAM APC, red-filled) from FORL2 transfekted BW5147 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Specific Publications For Anti-FOLR2 Purified Monoclonal Antibody (M04574)
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