Anti-Forkhead box protein O4 FOXO4 Antibody

Product Name

Anti-Forkhead box protein O4 FOXO4 Antibody

View all FOXO4 Antibodies

SKU/Catalog Number

A01819

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Forkhead box protein O4 FOXO4 Antibody (Catalog # A01819). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

FOXO4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Forkhead box protein O4 FOXO4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01819)

Host

Rabbit

Contents

FOXO4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

FOXO4 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human FOXO4. The immunogen is located within amino acids 30 - 80 of FOXO4.

Reactive Species

A01819 is reactive to FOXO4 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

53.7 kDa

Background of FOXO4

FOXO4 is a ubiquitously expressed protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In the absence of PI3K/AKT activation, FOXO4 localizes in the nucleus where it functions as a transcription factor. FOXO4 can also be phosphorylated by JNK following induction of reactive oxygen species (ROS), resulting in transcriptional activation and the induction of a negative feedback mechanism to counteract the ROS. It is through this mechanism that FOXO4 is thought to sensitize cancer cells to doxorubicin-mediated toxicity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01819 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FOXO4 antibody can be used for detection of FOXO4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information FOXO4

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