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Product Info Summary
| SKU: | A00964-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-MyoD1 Antibody (Center)
SKU/Catalog Number
A00964-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-MyoD1 Antibody (Center) (Catalog # A00964-1). Tested in IF, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MyoD1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00964-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MyoD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human MyoD1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00964-1 is reactive to MYOD1 in Human
Calculated molecular weight
34.5 kDa
Background of MYOD1
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00964-1 is guaranteed for IF, WB Boster Guarantee
Recommend Dilution
IF: 1:25
WB: 1:4000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling MYOD1 with A00964-1 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (red). The nuclear counter stain is DAPI (blue).
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All lanes : Anti-MyoD1 Antibody (Center) at 1:4000 dilution
Lane 1: Human skeletal muscle tissue lysate
Lane 2: Human placenta tissue lysate
Lane 3: RD whole cell lysate
Lane 4: HL-60 whole cell lysate
Lane 5: Hela whole cell lysate
Lane 6: HT-1080 whole cell lysate
Lane 7: U-2OS whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 35 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-MyoD1 Antibody (Center) (A00964-1)
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