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- Table of Contents
FOXF1 antibody (A03563-1) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of Jurkat cells using anti-FOXF1 antibody (A03563-1).
Overlay histogram showing Jurkat cells stained with A03563-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXF1 Antibody (A03563-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing Jurkat cells stained with A03563-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXF1 Antibody (A03563-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-FOXF1 Picoband® Antibody
FOXF1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
FOXF1 antibody was positively detected by WB in:
human A549 whole cell, human Hela whole cell, human PC-3 whole cell, human U2OS whole cell, mouse liver tissue, mouse SP20 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FOXF1 antibody was positively detected by IHC in:
human oesophagus squama cancer tissue, human rectal cancer tissue, rat lung tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
FOXF1 antibody was positively detected by FC in:
Jurkat cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Rat
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-FOXF1 Picoband® Antibody
View all FOXF1 Antibodies
View all FOXF1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FOXF1 recombinant protein (Position: R44-M379).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38-40 kDa
Calculated Molecular Weight
40.1 kDa
Background of FOXF1
Forkhead box protein F1 is a protein that in humans is encoded by the FOXF1 gene. It is mapped to 16q24.1. This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the regulation of pulmonary genes as well as embryonic development.
Cite This Product
Anti-FOXF1 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03563-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Sheep
97%
High Homology
Cow
96%
High Homology
Dog
96%
High Homology
Rabbit
96%
High Homology
Mouse
95%
High Homology
Rat
95%
High Homology
Zebrafish
81%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FOXF1 antibody
Immunohistochemistry (IHC) protocol for FOXF1 antibody
Flow Cytometry (FC) protocol for FOXF1 antibody
Publications
Application:
Species:
FOXF1 and SHH participate in the regulation of iron signaling in pulmonary fibrosis
Published: 2025-10-10
Journal: Redox Biology
Application: IHC,IF
Impact Factor: 11.90
Species: Mouse
PMID: 41101211
LINC00022 acts as an oncogene in colorectal cancer progression via sponging miR-375-3p to regulate FOXF1 expression
Published: 2022-04-26
Journal: BMC CANCER
Application: WB
Impact Factor: 4.64
Species: Human
PMID: 35468741
Review
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Product Promise
We promise Anti-FOXF1 Picoband® Antibody performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A03563-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026