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WNT7B antibody (A05086-2) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of THP-1 cells using anti-WNT7B antibody (A05086-2).
Overlay histogram showing THP-1 cells stained with A05086-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-WNT7B Antibody (A05086-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with A05086-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-WNT7B Antibody (A05086-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-WNT7B Antibody Picoband®
WNT7B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
WNT7B antibody was positively detected by WB in:
human HEK293 whole cell, human HELA whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
WNT7B antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Monkey
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Dog
Predicted
Predicted
Product Information
Product Name
Anti-WNT7B Antibody Picoband®
View all WNT7B Antibodies
View all WNT7B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human WNT7B recombinant protein (Position: K220-D267).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated Molecular Weight
39.3 kDa
Background of WNT7B
Protein Wnt-7b is a protein that in humans is encoded by the WNT7B gene. This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Among members of the human WNT family, this gene product is most similar to WNT7A protein.
Cite This Product
Anti-WNT7B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05086-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Monkey
100%
High Homology
Cow
98%
High Homology
Sheep
98%
High Homology
Dog
98%
High Homology
Zebrafish
75%
Moderate Homology
Drosophila
33%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for WNT7B antibody
Flow Cytometry (FC) protocol for WNT7B antibody
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Product Promise
We promise Anti-WNT7B Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A05086-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026