Anti-FoxO1a Rabbit Monoclonal Antibody

Western blot analysis of FOXO1 using anti-FOXO1 antibody (M00073).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO1 antigen affinity purified monoclonal antibody (M00073) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FOXO1 at approximately 80 kDa. The expected band size for FOXO1 is at 70 kDa.

The FXR agonist GW4064 improved fasting blood glucose and inhibited the translocation of PGC1α and FOXO1 from the nucleus to the cytoplasm in gluconeogenesis with FK506 treatment in mice for 3 months. a After FK506 was administered at a dose of 1 mg/kg/day to the C57BL/6J mice for 3 months, the fasting blood glucose levels obviously increased gradually compared with those of the control group, and the FXR agonist GW4064 downregulated the blood glucose levels. No significant difference was found for b body weights and c morphological alterations among the control group, the FK506 group and the FK506 + GW4064 group. The C-1, C-2 and C-3 groups were the control group, the FK506 group and the FK506 + GW4064 group, respectively. d , f – h , j , k Western blotting and quantitative analysis show the protein subcellular localization of PGC1α and FOXO1. Original magnification, ×3400 in each group. e , i IF staining for PGC1α ( e ) and FOXO1 ( i ) in sections of 3 groups. DAPI was used to locate the nuclei of the cells. Data are presented as the mean ± SD (n = 7). * P < 0.05 vs. the control group, # P < 0.05 vs. the FK506 group
Index in PubMed under a CC BY license. PMID: 31836014

Immunohistochemical analysis of paraffin-embedded human brain carcinoma, using FoxO1a Antibody.

Immunofluorescent analysis of Hela cells, using FoxO1a Antibody .