Anti-FoxO1a Rabbit Monoclonal Antibody

Boster Bio Anti-FoxO1a Rabbit Monoclonal Antibody catalog # M00073. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M00073
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-FoxO1a Rabbit Monoclonal Antibody

See all FOXO1 products

SKU/Catalog Number

M00073

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-FoxO1a Rabbit Monoclonal Antibody catalog # M00073. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FoxO1a Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00073)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CIA-6

Immunogen

A synthesized peptide derived from human FoxO1a

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00073 is reactive to FOXO1 in Human, Mouse, Rat

Applications

M00073 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For FOXO1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q12778

Gene ID

2308

Gene Name

FOXO1

Full Name

Forkhead box protein O1

Weight

69.662kDa

Alternative Names

FKH1; FKHR; FKHRforkhead box protein O1; forkhead box O1; Forkhead box protein O1A; forkhead homolog in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma; FoxO1; FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma FOXO1 FKH1, FKHRA, FOXO1 forkhead box O1 forkhead box protein O1|forkhead box protein O1A|forkhead, Drosophila, homolog of, in rhabdomyosarcoma

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FOXO1, check out the FOXO1 Infographic

FOXO1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FOXO1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00073 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li L,Zhao H,Chen B,Fan Z,Li N,Yue J,Ye Q.FXR activation alleviates tacrolimus-induced post-transplant diabetes mellitus by regulating renal gluconeogenesis and glucose uptake.J Transl Med.2019 Dec 13;17(1):418.doi:10.1186/s12967-019-02170-5.PMID:31836014;PMCID:PMC6909577.
Species: Mouse
M00073 usage in article: APP:IF, SAMPLE: KIDNEY TISSUE, DILUTION:NA

Dong P, Mai Y, Zhang Z, Mi L, Wu G, Chu G, Yang G, Sun S. Acta Biochim Biophys Sin (Shanghai). 2014 Jul;46(7):565-71. Doi: 10.1093/Abbs/Gmu043. Epub 2014 May 26. Mir-15A/B Promote Adipogenesis In Porcine Pre-Adipocyte Via Repressing Foxo1.

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16 Customer Q&As for Anti-FoxO1a Rabbit Monoclonal Antibody

Question

We bought anti-FoxO1a Rabbit Monoclonal antibody for IHC on rhabdomyosarcoma a few months ago. I am using human, and I plan to use the antibody for WB next. I was wanting to use examining rhabdomyosarcoma as well as lymph placenta in our next experiment. Could give a recommendation on which antibody would work the best for WB?

G. Wu

Verified customer

Asked: 2020-05-06

Answer

I have checked the website and datasheets of our anti-FoxO1a Rabbit Monoclonal antibody and it appears that M00073 has been tested on human in both IHC and WB. Thus M00073 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-05-06

Question

I was wanting to use using your anti-FoxO1a Rabbit Monoclonal antibody for regulation of neural precursor cell proliferation studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

I appreciate your inquiry. This M00073 anti-FoxO1a Rabbit Monoclonal antibody is validated on hela cells. It is guaranteed to work for IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-13

Question

We have observed staining in rat female gonad. Are there any suggestions? Is anti-FoxO1a Rabbit Monoclonal antibody supposed to stain female gonad positively?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

From literature female gonad does express FOXO1. From Uniprot.org, FOXO1 is expressed in female gonad, rhabdomyosarcoma, lymph placenta, cervix carcinoma, among other tissues. Regarding which tissues have FOXO1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Lymph, and Placenta, Pubmed ID: 15489334
Rhabdomyosarcoma, Pubmed ID: 9479491

Boster Scientific Support

Answered: 2020-02-19

Question

Will anti-FoxO1a Rabbit Monoclonal antibody M00073 work for ICC with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

According to the expression profile of cervix carcinoma, FOXO1 is highly expressed in cervix carcinoma. So, it is likely that anti-FoxO1a Rabbit Monoclonal antibody M00073 will work for ICC with cervix carcinoma.

Boster Scientific Support

Answered: 2019-12-24

Question

Our team were satisfied with the WB result of your anti-FoxO1a Rabbit Monoclonal antibody. However we have observed positive staining in rhabdomyosarcoma cytoplasm. using this antibody. Is that expected? Could you tell me where is FOXO1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

Based on literature, rhabdomyosarcoma does express FOXO1. Generally FOXO1 expresses in cytoplasm. Regarding which tissues have FOXO1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Lymph, and Placenta, Pubmed ID: 15489334
Rhabdomyosarcoma, Pubmed ID: 9479491

Boster Scientific Support

Answered: 2019-09-03

Question

Is this M00073 anti-FoxO1a Rabbit Monoclonal antibody reactive to the isotypes of FOXO1?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

The immunogen of M00073 anti-FoxO1a Rabbit Monoclonal antibody is A synthesized peptide derived from human FoxO1a. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-26

Question

I would like to test anti-FoxO1a Rabbit Monoclonal antibody M00073 on rat cervix carcinoma for research purposes, then I may be interested in using anti-FoxO1a Rabbit Monoclonal antibody M00073 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

The products we sell, including anti-FoxO1a Rabbit Monoclonal antibody M00073, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-31

Question

I was wanting to use your anti-FoxO1a Rabbit Monoclonal antibody for ICC for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

It shows on the product datasheet, M00073 anti-FoxO1a Rabbit Monoclonal antibody has been tested for IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-24

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-FoxO1a Rabbit Monoclonal antibody M00073. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-11-01

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-01

Question

I see that the anti-FoxO1a Rabbit Monoclonal antibody M00073 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-09-14

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-09-14

Question

Is a blocking peptide available for product anti-FoxO1a Rabbit Monoclonal antibody (M00073)?

Verified Customer

Verified customer

Asked: 2017-08-22

Answer

We do provide the blocking peptide for product anti-FoxO1a Rabbit Monoclonal antibody (M00073). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-08-22

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-FoxO1a Rabbit Monoclonal antibody M00073. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-07-10

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-10

Question

My question regarding product M00073, anti-FoxO1a Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

W. Jackson

Verified customer

Asked: 2017-05-15

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00073 anti-FoxO1a Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-05-15

Question

We are currently using anti-FoxO1a Rabbit Monoclonal antibody M00073 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

N. Patel

Verified customer

Asked: 2014-10-29

Answer

The anti-FoxO1a Rabbit Monoclonal antibody (M00073) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-10-29

Question

Is there a BSA free version of anti-FoxO1a Rabbit Monoclonal antibody M00073 available?

B. Mangal

Verified customer

Asked: 2014-07-22

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FoxO1a Rabbit Monoclonal antibody M00073 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-07-22

Question

Would M00073 anti-FoxO1a Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

K. Edwards

Verified customer

Asked: 2013-01-02

Answer

You can see on the product datasheet, M00073 anti-FoxO1a Rabbit Monoclonal antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-01-02

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
Order Product
M00073
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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