Forkhead box protein O1 Antibodies

Forkhead box protein O1 (FOXO1)

Transcription factor that's the main goal of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the associated Daf-16 household binding component (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'.

Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass.

Gene Information

Human
Gene Name:	FOXO1
Uniprot:	Q12778
Entrez:	2308

Family

Belongs to:
No superfamily

Alternative Names

FKH1; FKHR; FKHRforkhead box protein O1; forkhead box O1; Forkhead box protein O1A; forkhead homolog in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma; FoxO1; FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Molecular Weight

Mass (kDA):
69.662 kDA

Genomic Context

Human
Location:	13q14.11
Sequence:	13; NC_000013.11 (40555667..40666641, complement)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Cytoplasm. Nucleus. Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells. In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway. Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation. Phosphorylation by NLK results in nuclear export. Translocates to the nucleus upo

Diseases

• Neoplasms
• Malignant Neoplasms
• Diabetes Mellitus
• Insulin Resistance
• Rhabdomyosarcoma
• Alveolar Rhabdomyosarcoma
• Obesity
• Diabetes Mellitus, Non-insulin-dependent
• Cell Transformation, Neoplastic
• Chromosomal Translocation
• Atrophy
• Muscular Atrophy
• Insulin Sensitivity

• Hyperglycemia
• Inflammation
• Hyperplasia
• Carcinoma
• Liver Carcinoma
• Rhabdomyosarcoma, Embryonal
• Tumor Angiogenesis

Interacting Proteins

• AKT1
• CCNB1
• CDK1
• CREBBP
• ESR1

• FHL2
• SET
• SIRT1
• Sirt1
• YWHAZ

Pathways

• Cell Cycle
• Localization
• Cell Death
• Cell Proliferation
• Gluconeogenesis
• Secretion
• Aging
• Pathogenesis
• Muscle Atrophy
• Insulin Secretion
• Cell Cycle Arrest
• Glucose Homeostasis
• Cell Growth

• Angiogenesis
• Cell Differentiation
• Nuclear Export
• Transport
• Proteolysis
• Energy Homeostasis
• Decidualization

PTMs

• Phosphorylation
• Acetylation
• Dephosphorylation
• Oxidation
• Deacetylation
• Cleavage
• Ubiquitination
• Methylation
• Glycosylation
• Reduction

• Deglycosylation
• Farnesylation
• Sumoylation
• Demethylation
• Nitrosylation
• Prenylation
• Acylation
• Nitration
• Gamma-carboxylation
• Myristoylation

Research Areas

• Apoptosis
• DNA Repair
• Growth and Development
• Neuronal Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/FOXO1

Boster Bio - Best Uses Of The FOXO1 Marker

This article will discuss some of the most useful molecular biology techniques available with Boster Bio's FOXO1 marker, including Statistical analysis of FOXO1 and PAX3 expression data, VMO1 detection, and more. We'll also talk about the application of the FOXO1 marker to various diseases. These techniques can be very useful for researchers attempting to understand the origins of certain diseases and for research that aims to understand the mechanism of disease and treatment.

Boster Bio's molecular biology techniques

The new findings demonstrate that the human definitive endoderm gene FoxO1 plays a role in the regulation of cell growth and development. Researchers at Boster Bio used LCM and tissue disruption techniques to isolate pure populations of cell clones. The alteration of ERAD genes may explain the effect of FoxO on mutant cells. The discovery of this novel gene will be of interest to cell growth researchers around the world.

A DNA probe containing the Foxo1 marker was used to determine its interactions with nuclear proteins. This probe was designed to bind to three potential binding sites on the Runx2 promoter. Three unlabeled oligonucleotides were designed for these three sites and used with nuclear proteins. These proteins were then incubated with a 100-fold excess of these probes.

Statistical analyses of FOXO1 and PAX3 expression data

Statistical analyses of FOXO1 andPAX3 expression data were performed on a sample of EOC patients. Interestingly, high expression of FOXO1 and PAX3 was associated with poorer overall survival and a higher risk of death. These findings highlight the potential for these two genes as therapeutic and prognostic markers in cancer. Further research is needed to clarify their exact roles in the body.

A tissue microarray was constructed by using a tumor specimen from a patient with a tumor that contained sufficient amounts of the tumor. These tumor tissues were formalin-fixed and embedded in graded ethanol. After the sections were prepared, heat-induced antigen retrieval was performed. For each marker, the sample was incubated in a specific target retrieval buffer. For FOXO1 and PAX3, the reagent was diluted to make it non-toxic.

The PAX3-FOXO1 fusion gene product has the ability to regulate the expression of several proteins in ARMS. Furthermore, it has the potential to elucidate the role of PAX3-FOXO1 fusion genes in ARMS. Finally, the fusion gene product regulates the expression of PP2A, a potential therapeutic target in ARMS.

For the study, four ARMS cell lines were transfected with siRNA targeting PAX3-FOXO1 in a knockdown assay. Interestingly, all four cell lines were significantly less responsive to siRNA than the control groups. Protein expression was verified by Western blotting and the knockdown cells had significantly lower FOXO1 protein expression than the controls. The analysis results show that PAX3 and FOXO1 expression levels are associated with cellular activity. Understanding these interactions can lead to cancer therapies.

These transcription factors have important roles in glucose metabolism, cell cycle progression, apoptosis, and differentiation. However, their functions are not yet fully understood. Recent studies have shown that FOXO1 may act as a tumor suppressor in various cancer types, including EOC. But this is not enough to determine if they play a prognostic role. Further research is needed to better understand the mechanisms behind their action.

The results of this study indicate that FOXO1 expression levels are associated with colon formation and migration in EOC cells. Knockdown of FOXO1 significantly reduces colony formation compared to siRNA-transfected cells. Likewise, the knockdown of PAX3 decreased the viability of cells, as indicated by lower colony formation. The significance level was 0.05 or below.

In addition, the P3F oncoprotein was associated with aggressiveness in RMS. Furthermore, PAX3/FOXO1 fusion status significantly improved the current risk stratification in patients with this type of cancer. These studies provide further support for the importance of biomarkers and effective therapeutic targets for this disease. The role of these genes in tumor growth and differentiation is emphasized in RMS.

Detection of VMO1 with FOXO1 Marker

Detection of VMO1 using the FOXO1 marker was performed in a cell line that expresses the mRNA for this gene. In this study, OVCA429 and OVCA433 cells were used. Cells were homogenized in RIPA buffer and treated with a proteinase inhibitor cocktail. Then, protein levels were measured using SDS-PAGE.

In addition to its role in cardiomyocyte hypertrophy, FOXO1 also plays an important role in cardiac remodeling. This protein is required for the production of insulin and is involved in the regulation of EC apoptosis and viability. Its expression is upregulated in tumors and cardiovascular diseases. Its expression increases in response to stress or serum deprivation.

Activated FOXO1 expression promotes differentiation and homeostasis in MPECs. It is also essential for cellular redox. It is induced by Akt-mediated phosphorylation. Further, increased Foxo1 expression may be indicative of a disease or condition affecting MPECs. In addition to regulating homeostasis, it is a good marker for detecting vascular malformation.

Expression of PAX3 and FOXO1 is a significant indicator of poor prognosis. This suggests that FOXO1 and PAX3 are potential therapeutic and prognostic markers for EOC. Further study is needed to determine how these genes regulate different biological processes. But for now, this marker may help with the diagnosis of various types of cancers. If you want to detect VMO1 in the blood, the FOXO1 and PAX3 gene expression may help you with your search.

Using a log2-fold change, a similar signal was obtained in the two independent datasets. MPEC and SLEC were determined based on log2-fold-change. For the analysis of the two gene expression levels, we used a log2-fold-change. In addition to this, we used a standard amplification procedure that was performed for the VMO1 protein marker.

Activated memory T cells respond well to antigen-experienced CD8+ T cells. Foxo1 is a critical component of the immune system, promoting memory T cell migration and homeostasis in vivo. Moreover, Foxo1 has an anti-inflammatory role and plays a key role in the development of autoimmune diseases. Further research is needed to understand how Foxo1 regulates T cell homeostasis.