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Phb2 antibody (DZ41317) performs well in IF, WB and shows reactivity with Fruit fly.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of Phb2 using anti-Phb2 antibody (DZ41317).
Phb2 was detected in a paraffin-embedded section of Drosophila ovary tissue. The tissues were fixed with 4% PFA and permeabilized in PBS-TX. And then incubated with 1:100 rabbit anti-Phb2 Antibody (DZ41317) in 2% BSA and 0.1% PBS-TX overnight at 4°C. Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5 was used as secondary antibody at 1:1000 dilution and incubated for 1 hours at RT. Visualize using Zeiss LSM 900 Confocal Microscope with Airyscan 2 and filter sets appropriate for the label used.
Phb2 was detected in a paraffin-embedded section of Drosophila ovary tissue. The tissues were fixed with 4% PFA and permeabilized in PBS-TX. And then incubated with 1:100 rabbit anti-Phb2 Antibody (DZ41317) in 2% BSA and 0.1% PBS-TX overnight at 4°C. Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5 was used as secondary antibody at 1:1000 dilution and incubated for 1 hours at RT. Visualize using Zeiss LSM 900 Confocal Microscope with Airyscan 2 and filter sets appropriate for the label used.
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Anti-Fruit fly Phb2 Antibody
Phb2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Fruit fly | Rabbit | IF, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1:1000
IF: 1:100
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Fruit fly Phb2 Antibody
View all PHB2 Antibodies
View all PHB2 Antibodies
Form
Liquid
Uniprot
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Cite This Product
Anti-Fruit fly Phb2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41317)
Review
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2 Reviews For Anti-Fruit fly Phb2 Antibody
Western blot analysis of Drosophila ovaries from three fly strains showed comparable Phb2 protein levels across all samples. Consistent bands at 30 kDa confirmed expected molecular weight, with β-Actin used as a loading control to verify equal protein loa
| SKU | DZ41317 |
|---|---|
| Application | Western Blot |
| Sample | Drosophila Ovaries |
| Sample Processing Description | Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting |
| Primary Antibody | Anti-Fruit fly Phb2 Antibody |
| Primary Incubation | 1:1000 in 5% BSA and 0.1% PBST. Either 2 hours at RT or Overnight at 4 Degrees |
| Secondary Antibody | 1:10000 Anti-rabbit/mouse IgG horseradish peroxidase-conjugated |
| Secondary Incubation | 1 hour at room temperature |
| Detection | Biorad ChemiDoc |
| Results Summary | Western blot analysis of Drosophila ovaries from three fly strains showed comparable Phb2 protein levels across all samples. Consistent bands at 30 kDa confirmed expected molecular weight, with β-Actin used as a loading control to verify equal protein loading. |
Kasturi Mitra
Verified customer
Submitted 2026-01-07
Drosophila fatbody showed good staining of Phb2.
| SKU | DZ41317 |
|---|---|
| Application | Immunofluorescence |
| Sample | Drosophila Fat body and Ovaries |
| Sample Processing Description | Dissection, Fixation in 4%PFA, Permeabilization in PBS-TX, Blocking, Primary, Washes,Secondary, Washes,Nuclei Staining, Slide Preparation |
| Primary Antibody | Anti-Fruit fly Phb2 Antibody |
| Primary Incubation | 1:200 in 2%BSA and 0.5% PBS-TX for Ovaries, 1:100 in 2%BSA and 0.1% PBS-TX for Fat body |
| Secondary Antibody | 1:1000 Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5 |
| Secondary Incubation | 1 hour at room temperature |
| Detection | Zeiss LSM 900 Confocal Microscope with Airyscan 2 |
| Results Summary | Drosophila fatbody showed good staining of Phb2. |
Kasturi Mitra
Verified customer
Submitted 2026-01-07
Product Promise
We promise Anti-Fruit fly Phb2 Antibody performs excellently in relevant applications for IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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DZ41317
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026