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Product Info Summary
| SKU: | A06754-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-G beta 5 GNB5 Antibody
SKU/Catalog Number
A06754-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-G beta 5 GNB5 Antibody catalog # A06754-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-G beta 5 GNB5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06754-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GNB5. AA range:151-200
Reactive Species
A06754-1 is reactive to GNB5 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
43.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06754-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of HepG2 cells using Gβ 5 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Specific Publications For Anti-G beta 5 GNB5 Antibody (A06754-1)
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6 Customer Q&As for Anti-G beta 5 GNB5 Antibody
Question
We are currently using anti-G beta 5 antibody A06754-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-G beta 5 antibody (A06754-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-G beta 5 antibody A06754-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-08
Question
My question regarding product A06754-1, anti-G beta 5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06754-1 anti-G beta 5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-12
Question
I was wanting to use to test anti-G beta 5 antibody A06754-1 on rat lymph for research purposes, then I may be interested in using anti-G beta 5 antibody A06754-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-05-23
Answer
The products we sell, including anti-G beta 5 antibody A06754-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-23
Question
I was wanting to use your anti-G beta 5 antibody for WB for rat lymph on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat lymph identification?
E. Wu
Verified customer
Asked: 2016-05-13
Answer
It shows on the product datasheet, A06754-1 anti-G beta 5 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lymph in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-13
Question
Is a blocking peptide available for product anti-G beta 5 antibody (A06754-1)?
J. Huang
Verified customer
Asked: 2015-03-17
Answer
We do provide the blocking peptide for product anti-G beta 5 antibody (A06754-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-03-17
