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AQP4 antibody (PA1937) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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IHC analysis of AQP4 using anti-AQP4 antibody (PA1937).
AQP4 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP4 Antibody (PA1937) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
AQP4 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP4 Antibody (PA1937) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Aquaporin 4/AQP4 Antibody Picoband®
AQP4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
AQP4 antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
AQP4 antibody was positively detected by IHC in:
human glioma tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Human
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Aquaporin 4/AQP4 Antibody Picoband®
View all AQP4 Antibodies
View all AQP4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4, different from the related rat and mouse sequences by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
24 kDa
Calculated Molecular Weight
34.8 kDa
Background of AQP4
AQP4 (Aquaporin 4), also known as MERCURIAL-INSENSITIVE WATER CHANNEL; MIWC, is protein which in humans is encoded by the AQP4 gene. The aquaporins are a family of water-selective membrane channels found in animals, plants, and microorganisms. AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. By fluorescence in situ hybridization, Lu et al. (1996) determined that the AQP4 gene maps to 18q11.2-q12.1. By interspecific backcross analysis, Turtzo et al. (1997) mapped the mouse Aqp4 gene to the proximal region of chromosome 18. Analyzing the expression of AQP4 in mammalian skeletal muscle, Frigeri et al. (1998) found that, in immunohistochemical experiments, affinity-purified AQP4 antibodies stained selectively the sarcolemma of fast-twitch fibers. Immunocytochemistry revealed strong AQP4 water channel expression in Muller cells in mouse retina and in fibrous astrocytes in optic nerve.
Cite This Product
Anti-Aquaporin 4/AQP4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1937)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
94%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Monkey
94%
High Homology
Mouse
88%
Moderate Homology
Rat
88%
Moderate Homology
Dog
82%
Moderate Homology
Zebrafish
35%
Low homology
Drosophila
29%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for AQP4 antibody
Immunohistochemistry (IHC) protocol for AQP4 antibody
Publications
Application:
Species:
Euphorbia factor L1 inhibited transport channel and energy metabolism in human colon adenocarcinoma cell line Caco-2
Published: 2023-11-22
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 7.50
Species: Human
PMID: 37992574
Acetazolamide Alleviate Cerebral Edema Induced by Ischemic Stroke Through Inhibiting the Expression of AQP4 mRNA
Published: 2021-07-23
Journal: Neurocritical Care
Application: IHC
Impact Factor: 3.21
Species: Rat
PMID: 34302276
Orientin Attenuates Cerebral Ischemia/Reperfusion Injury in Rat Model through the AQP-4 and TLR4/NF-κB/TNF-α Signaling Pathway
Published: 2017-06-20
Journal: Journal of Stroke & Cerebrovascular Diseases
Application: IHC
Impact Factor: 1.52
PMID: 28645524
Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema
Published: 2015-08-11
Journal: Molecular Medicine Reports
Application: IHC
Impact Factor: 1.55
Species: Human
PMID: 26261083
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Product Promise
We promise Anti-Aquaporin 4/AQP4 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1937
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026