Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody

Product Name

Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody

View all Glucose 6 Phosphate Dehydrogenase Antibodies

SKU/Catalog Number

M00287

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody catalog # M00287. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00287)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABBI-7

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human G6PD

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00287 is reactive to G6PD in Human

Applications

M00287 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

59 kDa

Calculated molecular weight

59.257kDa

Background of Glucose 6 Phosphate Dehydrogenase

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of G6PD using anti-G6PD antibody (M00287).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G6PD antigen affinity purified monoclonal antibody (Catalog # M00287) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for G6PD at approximately 59 kDa. The expected band size for G6PD is at 59 kDa.

Gene/Protein Information For G6PD (Source: Uniprot.org, NCBI)

Alternative Names

G6PD; G6PD1EC 1.1.1.49; G6PDH; glucose-6-phosphate dehydrogenase G6PD G6PD1 glucose-6-phosphate dehydrogenase glucose-6-phosphate 1-dehydrogenase|epididymis secretory sperm binding protein

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