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Product Info Summary
| SKU: | M00287 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody
SKU/Catalog Number
M00287
BM5109 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody catalog # M00287. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00287)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABBI-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human G6PD
Reactive Species
M00287 is reactive to G6PD in Human
Observed Molecular Weight
59 kDa
Calculated molecular weight
59.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00287 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human MCF-7 whole cell, human A549 whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Western blot analysis of G6PD using anti-G6PD antibody (M00287).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G6PD antigen affinity purified monoclonal antibody (Catalog # M00287) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for G6PD at approximately 59 kDa. The expected band size for G6PD is at 59 kDa.
Specific Publications For Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody (M00287)
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6 Customer Q&As for Anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal Antibody
Question
I see that the anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal antibody M00287 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-22
Question
Will anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal antibody M00287 work on dog IF with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Our lab technicians have not validated anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal antibody M00287 on dog. You can run a BLAST between dog and the immunogen sequence of anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal antibody M00287 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cervix carcinoma in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-24
Question
We want to test anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal antibody M00287 on human cervix carcinoma for research purposes, then I may be interested in using anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal antibody M00287 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-15
Answer
The products we sell, including anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal antibody M00287, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal antibody M00287. Let me know if you need anything else.
P. Carter
Verified customer
Asked: 2017-12-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-12
Question
Would M00287 anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-18
Answer
It shows on the product datasheet, M00287 anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-18
Question
Is this M00287 anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal antibody reactive to the isotypes of G6PD?
K. Anderson
Verified customer
Asked: 2013-04-29
Answer
The immunogen of M00287 anti-G6PD/Glucose 6 Phosphate Dehydrogenase Rabbit Monoclonal antibody is A synthesized peptide derived from human G6PD. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-04-29
