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Product Info Summary
| SKU: | M04681 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IP, IF, IHC, WB |
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Product info
Product Name
Anti-GADD45G Mouse Monoclonal Antibody [Clone ID: OTI2F12]
SKU/Catalog Number
M04681
Size
100 µl
Description
Boster Bio GADD45G mouse monoclonal antibody, clone OTI2F12 (formerly 2F12). Catalog# M04681. Tested in IF, IHC, IP, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-GADD45G Mouse Monoclonal Antibody [Clone ID: OTI2F12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04681)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2F12
Isotype
IgG1
Immunogen
Full length human recombinant protein of human GADD45G (NP_006696) produced in HEK293T cell.
Reactive Species
M04681 is reactive to GADD45G in Human, Mouse, Rat
Calculated molecular weight
17.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04681 is guaranteed for IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:4000
IHC 1:150
IF 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
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Figure from citation: Western Blot of GADD45G protein level by using anti-GADD45G antibody in human NPC cell lines.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Anti-GADD45G mouse monoclonal antibody (M04681) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD45G.
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Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human NPC cell lines.
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Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human GBM8401 cells.
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Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human CRC cells.
Specific Publications For Anti-GADD45G Mouse Monoclonal Antibody [Clone ID: OTI2F12] (M04681)
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