Anti-GADD45G Antibody Picoband™

GADD45G antibody

Boster Bio Anti-GADD45G Antibody Picoband™ catalog # A04681-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A04681-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-GADD45G Antibody Picoband™

View all GADD45G Antibodies

SKU/Catalog Number

A04681-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GADD45G Antibody Picoband™ catalog # A04681-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GADD45G Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04681-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human GADD45G, which shares 94.4% and 100% amino acid (aa) sequence identity with mouse and rat GADD45G, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04681-1 is reactive to GADD45G in Human, Mouse, Rat

Applications

A04681-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

17.121kDa

Background of GADD45G

Growth arrest and DNA-damage-inducible protein GADD45 gamma is a protein that in humans is encoded by the GADD45G gene on chromosome 9. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For GADD45G (Source: Uniprot.org, NCBI)

Gene Name

GADD45G

Full Name

Growth arrest and DNA damage-inducible protein GADD45 gamma

Weight

17.121kDa

Superfamily

GADD45 family

Alternative Names

CR6DNA damage-inducible transcript 2 protein; Cytokine-responsive protein CR6; DDIT-2; DDIT2GADD45-gamma; growth arrest and DNA damage-inducible protein GADD45 gamma; growth arrest and DNA-damage-inducible, gamma; GRP1717 kD GADD45G CR6, DDIT2, GADD45gamma, GRP17 growth arrest and DNA damage inducible gamma growth arrest and DNA damage-inducible protein GADD45 gamma|DDIT-2|DNA damage-inducible transcript 2 protein|GADD45-gamma|cytokine-responsive protein CR6|gadd-related protein, 17 kD

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GADD45G, check out the GADD45G Infographic

GADD45G infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GADD45G: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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13 Customer Q&As for Anti-GADD45G Antibody Picoband™

Question

Is a blocking peptide available for product anti-GADD45G antibody (A04681-1)?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

We do provide the blocking peptide for product anti-GADD45G antibody (A04681-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-19

Question

Do you have a BSA free version of anti-GADD45G antibody A04681-1 available?

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GADD45G antibody A04681-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-25

Question

We are currently using anti-GADD45G antibody A04681-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

The anti-GADD45G antibody (A04681-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-15

Question

I was wanting to use your anti-GADD45G antibody for WB for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

It shows on the product datasheet, A04681-1 anti-GADD45G antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-13

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-GADD45G antibody A04681-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-03-15

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-03-15

Question

My question regarding product A04681-1, anti-GADD45G antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-01-15

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04681-1 anti-GADD45G antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-01-15

Question

Here is the WB image, lot number and protocol we used for lung using anti-GADD45G antibody A04681-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-12-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-12-13

Question

Is this A04681-1 anti-GADD45G antibody reactive to the isotypes of GADD45G?

Verified Customer

Verified customer

Asked: 2018-12-07

Answer

The immunogen of A04681-1 anti-GADD45G antibody is A synthetic peptide corresponding to a sequence of human GADD45G (MTLEEVRGQDTVPESTARMQGAGKALHELLLSAQRQ). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-12-07

Question

I am interested in to test anti-GADD45G antibody A04681-1 on human lung for research purposes, then I may be interested in using anti-GADD45G antibody A04681-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-12-05

Answer

The products we sell, including anti-GADD45G antibody A04681-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-12-05

Question

Would anti-GADD45G antibody A04681-1 work for WB with lung?

Verified Customer

Verified customer

Asked: 2018-10-04

Answer

According to the expression profile of lung, GADD45G is highly expressed in lung. So, it is likely that anti-GADD45G antibody A04681-1 will work for WB with lung.

Boster Scientific Support

Answered: 2018-10-04

Question

Will anti-GADD45G antibody A04681-1 work on dog WB with brain?

Verified Customer

Verified customer

Asked: 2018-08-24

Answer

Our lab technicians have not tested anti-GADD45G antibody A04681-1 on dog. You can run a BLAST between dog and the immunogen sequence of anti-GADD45G antibody A04681-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-24

Question

Will A04681-1 anti-GADD45G antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Krishna

Verified customer

Asked: 2017-10-02

Answer

As indicated on the product datasheet, A04681-1 anti-GADD45G antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-10-02

Question

I see that the anti-GADD45G antibody A04681-1 works with WB, what is the protocol used to produce the result images on the product page?

S. Li

Verified customer

Asked: 2016-08-10

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-08-10

Order DetailsPrice
A04681-1

100μg

$370
A04681-1-10ug

10μg sample (liquid)

$99
A04681-1-Biotin

100 μg Biotin conjugated

$570
A04681-1-Cy3

100 μg Cy3 conjugated

$570
A04681-1-Dylight488

100 μg Dylight488 conjugated

$570
A04681-1-Dylight550

100 μg Dylight550 conjugated

$570
A04681-1-Dylight594

100 μg Dylight594 conjugated

$570
A04681-1-FITC

100 μg FITC conjugated

$570
A04681-1-HRP

100 μg HRP conjugated

$570
A04681-1-APC

100 μg APC conjugated

$670
A04681-1-PE

100 μg PE conjugated

$670
A04681-1-carrier-free

Carrier Free

$370

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A04681-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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