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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||IP, ICC, WB|
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Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114
Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 catalog # M00227-5. Tested in IP, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-5)
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
A peptide corresponding to the C-terminus of GAPDH
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M00227-5 is reactive to GAPDH in Human, Mouse, Rat
M00227-5 is guaranteed for IP, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western Blot (WB): 1:1000 dilution;
Immunoprecipitation (IP): 1:200 dilution;
Chromatin IP (ChIP): 1:200 dilution;
Immunocytochemistry (ICC): 1:200 dilution;
Immunohistochemistry (IHC): 1:200 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western blot of A431 cells, using Clone RM114 at 1/1000 dilution. A GAPDH band showed at the predicted MW (~36 kDa).
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Figure 2. ICC result
Immunocytochemical staining of HeLa cells, using anti-GAPDH Clone RM114 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).
Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)
glyceraldehyde-3-phosphate dehydrogenase family
1D4 GAPDH; aging-associated gene 9 protein; Cytoplasm Marker; EC 1.2.1; EC 220.127.116.11; EC 2.6.99.-; G3PD; G3PD; G3PDH; GAPD; GAPDH; GAPDPeptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38; OCT1 coactivator in S phase; Peptidyl-cysteine S-nitrosylase GAPDH GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on GAPDH, check out the GAPDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
M00227-5 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
(-)-Epigallocatechin-3-gallate induces interferon-λ2 expression to anti-influenza A virus in human bronchial epithelial cells (BEAS-2B) through p38 MAPK signaling pathway
Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathy
Xie R,Liu J,Yu X,Li C,Wang Y,Yang W,Hu J,Liu P,Sui H,Liang P,Huang X,Wang L,Bai Y,Xue Y,Zhu J,Fang T.ANXA2 Silencing Inhibits Proliferation, Invasion, and Migration in Gastric Cancer Cells.J Oncol.2019 May 2;2019:4035460.doi:10.1155/2019/4035460.PMID:31186633;PMCID:PMC6521490.
M00227-5 usage in article: APP:WB, SAMPLE:SGC-7901 CELL,MKN-45 CELL,BGC-823 CELL AND AGS CELL, DILUTION:NA
Tibatan MA,Önay Uçar E.Long non-coding RNA NKILA regulates expression of HSP90α, NF-κB and β-catenin proteins in the MCF-7 breast cancer cell line.Mol Biol Rep.2021 Jun 14.doi:10.1007/s11033-021-06482-y.Epub ahead of print.PMID:34125329.
M00227-5 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:NA
Deng S,Zhang Y,Liao Z,Huang J,Huang R,Li Z.S100A4 plays a key role in TRPV3 ion channel expression and its electrophysiological function.Neurosci Lett.2021 May 28:135999.doi:10.1016/j.neulet.2021.135999.Epub ahead of print.PMID:34058292.
M00227-5 usage in article: APP:WB, SAMPLE:HEK293 T CELL, DILUTION:NA
Bin Wang,Jinyu Li,Mi Bao,Runji Chen,Haiyan Li,Binger Lu,Meixin Chen,Danmei Huang,Yanmei Zhang,Fenfei Gao,Ganggang Shi,"Melatonin Attenuates Diabetic Myocardial Microvascular Injury through Activating the AMPK/SIRT1 Signaling Pathway",Oxidative Medicine and Cellular Longevity,vol.2021,Article ID 8882130,15 pages,2021.https://doi.org/10.1155/2021/8882130
M00227-5 usage in article: APP:WB, SAMPLE:CMECS, DILUTION:NA
Chen X,Xu H,Hou J,Wang H,Zheng Y,Li H,Cai H,Han X,Dai J.Epithelial cell senescence induces pulmonary fibrosis through Nanog-mediated fibroblast activation.Aging (Albany NY).2019 Dec 31;12(1):242-259.doi:10.18632/aging.102613.Epub 2019 Dec 31.PMID:31891567
M00227-5 usage in article: APP:WB, SAMPLE:MLE-12 CELL, DILUTION:NA
She Y,Sun J,Hou P,Fang P, Zhang Z. Time-restricted feeding attenuates gluconeogenic activity through inhibition of PGC-1α expression and activity. Physiol Behav. 2021 Mar 15;231:113313. doi: 10.1016/j.physbeh.2021.113313. Epub 2021 Jan 4.PMID:33412190.
M00227-5 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Gang-Zhu Xu,Yang Xue,Si-Qi Wei,Jia-Heng Li,Richard J. Traub,Mao-De Wang,Dong-Yuan Cao,Valproate reverses stress-induced somatic hyperalgesia and visceral hypersensitivity by up-regulating spinal 5-HT2C receptor expression in female rats,Neuropharmacology,
M00227-5 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:4000
Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
M00227-5 usage in article: APP:WB, SAMPLE:MSC-LIPO, DILUTION:NA
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