Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114

GAPDH antibody

Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 catalog # M00227-5. Tested in IP, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: M00227-5
Size: 100uL
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, ICC, WB

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Product Name

Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114

View all GAPDH Antibodies

SKU/Catalog Number

M00227-5

Size

100uL

Description

Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 catalog # M00227-5. Tested in IP, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-5)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM114

Immunogen

A peptide corresponding to the C-terminus of GAPDH

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00227-5 is reactive to GAPDH in Human, Mouse, Rat

Applications

M00227-5 is guaranteed for IP, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western Blot (WB): 1:1000 dilution;
Immunoprecipitation (IP): 1:200 dilution;
Chromatin IP (ChIP): 1:200 dilution;
Immunocytochemistry (ICC): 1:200 dilution;
Immunohistochemistry (IHC): 1:200 dilution.

Validation Images & Assay Conditions

Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)

Uniprot ID

P04406

Gene ID

2597

Gene Name

GAPDH

Full Name

Glyceraldehyde-3-phosphate dehydrogenase

Weight

36.053kDa

Superfamily

glyceraldehyde-3-phosphate dehydrogenase family

Alternative Names

1D4 GAPDH; aging-associated gene 9 protein; Cytoplasm Marker; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PD; G3PDH; GAPD; GAPDH; GAPDPeptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38; OCT1 coactivator in S phase; Peptidyl-cysteine S-nitrosylase GAPDH GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GAPDH, check out the GAPDH Infographic

GAPDH infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

M00227-5 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

(-)-Epigallocatechin-3-gallate induces interferon-λ2 expression to anti-influenza A virus in human bronchial epithelial cells (BEAS-2B) through p38 MAPK signaling pathway

Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathy

Xie R,Liu J,Yu X,Li C,Wang Y,Yang W,Hu J,Liu P,Sui H,Liang P,Huang X,Wang L,Bai Y,Xue Y,Zhu J,Fang T.ANXA2 Silencing Inhibits Proliferation, Invasion, and Migration in Gastric Cancer Cells.J Oncol.2019 May 2;2019:4035460.doi:10.1155/2019/4035460.PMID:31186633;PMCID:PMC6521490.
Species: Human
M00227-5 usage in article: APP:WB, SAMPLE:SGC-7901 CELL,MKN-45 CELL,BGC-823 CELL AND AGS CELL, DILUTION:NA

Tibatan MA,Önay Uçar E.Long non-coding RNA NKILA regulates expression of HSP90α, NF-κB and β-catenin proteins in the MCF-7 breast cancer cell line.Mol Biol Rep.2021 Jun 14.doi:10.1007/s11033-021-06482-y.Epub ahead of print.PMID:34125329.
Species: Human
M00227-5 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:NA

Deng S,Zhang Y,Liao Z,Huang J,Huang R,Li Z.S100A4 plays a key role in TRPV3 ion channel expression and its electrophysiological function.Neurosci Lett.2021 May 28:135999.doi:10.1016/j.neulet.2021.135999.Epub ahead of print.PMID:34058292.
Species: Human
M00227-5 usage in article: APP:WB, SAMPLE:HEK293 T CELL, DILUTION:NA

Bin Wang,Jinyu Li,Mi Bao,Runji Chen,Haiyan Li,Binger Lu,Meixin Chen,Danmei Huang,Yanmei Zhang,Fenfei Gao,Ganggang Shi,"Melatonin Attenuates Diabetic Myocardial Microvascular Injury through Activating the AMPK/SIRT1 Signaling Pathway",Oxidative Medicine and Cellular Longevity,vol.2021,Article ID 8882130,15 pages,2021.https://doi.org/10.1155/2021/8882130
Species: Mouse
M00227-5 usage in article: APP:WB, SAMPLE:CMECS, DILUTION:NA

Chen X,Xu H,Hou J,Wang H,Zheng Y,Li H,Cai H,Han X,Dai J.Epithelial cell senescence induces pulmonary fibrosis through Nanog-mediated fibroblast activation.Aging (Albany NY).2019 Dec 31;12(1):242-259.doi:10.18632/aging.102613.Epub 2019 Dec 31.PMID:31891567
Species: Human,Mouse
M00227-5 usage in article: APP:WB, SAMPLE:MLE-12 CELL, DILUTION:NA

She Y,Sun J,Hou P,Fang P, Zhang Z. Time-restricted feeding attenuates gluconeogenic activity through inhibition of PGC-1α expression and activity. Physiol Behav. 2021 Mar 15;231:113313. doi: 10.1016/j.physbeh.2021.113313. Epub 2021 Jan 4.PMID:33412190.
Species: Mouse
M00227-5 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

Gang-Zhu Xu,Yang Xue,Si-Qi Wei,Jia-Heng Li,Richard J. Traub,Mao-De Wang,Dong-Yuan Cao,Valproate reverses stress-induced somatic hyperalgesia and visceral hypersensitivity by up-regulating spinal 5-HT2C receptor expression in female rats,Neuropharmacology,
Species: Rat
M00227-5 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:4000

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
M00227-5 usage in article: APP:WB, SAMPLE:MSC-LIPO, DILUTION:NA

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16 Customer Q&As for Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114

Question

See below the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-21

Question

Would anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 work for IP with cajal-retzius cell fetal brain cortex?

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

According to the expression profile of cajal-retzius cell fetal brain cortex, GAPDH is highly expressed in cajal-retzius cell fetal brain cortex. So, it is likely that anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 will work for IP with cajal-retzius cell fetal brain cortex.

Boster Scientific Support

Answered: 2020-03-20

Question

Does M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

It shows on the product datasheet, M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-30

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-20

Question

Is this M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 reactive to the isotypes of GAPDH?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

The immunogen of M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 is A peptide corresponding to the C-terminus of GAPDH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-19

Question

I was wanting to use your anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 for IP for mouse cajal-retzius cell fetal brain cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cajal-retzius cell fetal brain cortex identification?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

You can see on the product datasheet, M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 has been tested for IP, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cajal-retzius cell fetal brain cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-17

Question

I see that the anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 works with IP, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-08

Question

My lab would like using your anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 for killing by host of symbiont cells studies. Has this antibody been tested with western blotting on a431 cells? We would like to see some validation images before ordering.

E. Wu

Verified customer

Asked: 2019-06-28

Answer

I appreciate your inquiry. This M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 is tested on a431 cells, hela cells. It is guaranteed to work for IP, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-28

Question

I am interested in to test anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 on mouse cajal-retzius cell fetal brain cortex for research purposes, then I may be interested in using anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-04-26

Answer

The products we sell, including anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-04-26

Question

Do you have a BSA free version of anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 available?

Verified Customer

Verified customer

Asked: 2019-01-31

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-01-31

Question

Can you help my question with product M00227-5, anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-04-13

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-04-13

Question

Is a blocking peptide available for product anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 (M00227-5)?

Verified Customer

Verified customer

Asked: 2017-12-07

Answer

We do provide the blocking peptide for product anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 (M00227-5). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-12-07

Question

Our team were happy with the WB result of your anti-GAPDH Rabbit Monoclonal antibody, Clone

Z. Collins

Verified customer

Asked: 2016-09-12

Answer

From literature, leukemic t-cell does express GAPDH. Generally GAPDH expresses in cytoplasm, cytosol. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305

Boster Scientific Support

Answered: 2016-09-12

Question

We are currently using anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

S. Krishna

Verified customer

Asked: 2016-06-17

Answer

The anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 (M00227-5) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-06-17

Question

We have been able to see staining in human cajal-retzius cell fetal brain cortex. Any tips? Is anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 supposed to stain cajal-retzius cell fetal brain cortex positively?

K. Zhao

Verified customer

Asked: 2015-08-28

Answer

From what I have seen in literature cajal-retzius cell fetal brain cortex does express GAPDH. From what I have seen in Uniprot.org, GAPDH is expressed in smooth muscle tissue, liver, lung, placenta, astrocytoma, leukemia, colon adenocarcinoma, eye, kidney, lung, lymph placenta, muscle, platelet, prostatic carcinoma, brain, cajal-retzius cell fetal brain cortex, heart, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305

Boster Scientific Support

Answered: 2015-08-28

Question

We ordered your anti-GAPDH Rabbit Monoclonal antibody, Clone

K. Kulkarni

Verified customer

Asked: 2015-01-23

Answer

I have checked the website and datasheets of our anti-GAPDH Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2015-01-23

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M00227-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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