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GAPDH antibody (A00227-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Chicken, Human, Monkey, Mouse, Rat, Zebrafish.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 142 Publications

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a00227 1 gapdh primary antibodies fcm testing 6

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Flow Cytometry analysis of Hela cells using anti-GAPDH antibody (A00227-1).
Overlay histogram showing Hela cells stained with A00227-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GAPDH Antibody (A00227-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-GAPDH Antibody Picoband®

Name: Anti-GAPDH Antibody Picoband®
Brand: Boster Bio
SKU: A00227-1
Price: 370 USD
Availability: InStock
Rating: 100 (3 reviews)

GAPDH antibody

142 Publications|

3 Reviews

|Submit a Review

Reactive Species	Host	Application
Chicken, Human, Monkey, Mouse, Rat, Zebrafish	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

GAPDH antibody was positively detected by WB in:

human Hela whole cell, human CACO-2 whole cell, human CCRF-CEM whole cell, SK-HEP1 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

GAPDH antibody was positively detected by IHC in:

human laryngeal squamous cell carcinoma tissue, human renal clear cell carcinoma tissue, human ovarian serous cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Chicken

Recommend

Monkey

Recommend

Zebrafish

Recommend

Rabbit

Predicted

Dog

Predicted

Bovine

Predicted

Sheep

Predicted

Product Information

Product Name

Anti-GAPDH Antibody Picoband®
View all GAPDH Antibodies

Form

Lyophilized

Uniprot

P04406

Gene ID

2597

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human GAPDH recombinant protein (Position: N136-E335). Human GAPDH shares 95% and 94.5% amino acid (aa) sequence identity with mouse and rat GAPDH, respectively.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

36 kDa

Calculated Molecular Weight

36.1 kDa

Background of GAPDH

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus.

Cite This Product

Anti-GAPDH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00227-1)

Protocols

Western Blot protocol for GAPDH antibody

Review Protocol

Immunohistochemistry (IHC) protocol for GAPDH antibody

Review Protocol

Publications

Repeated Moderate and Severe Heat Exposure Has Divergent Effects on Skeletal Muscle Mitochondrial, Proteostatic and Neuromuscular Outcomes in Mice

Published: 2026-08-27

Journal: Acta Physiologica

Application: WB

Impact Factor: 5.70

Species: Mouse

PMID: 42656190

SKU	A00227-1
Application	Immunohistochemistry
Sample	Human SH-SY5Y cells
Sample Processing Description	Extract total cellular protein, denature it with loading buffer, and load 10 µg of the protein sample.
Other Reagents	Blocking solution (5% non-fat milk); BCA Protein Assay Kit
Primary Antibody	GAPDH Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	Anti-rabbit IgG HRP-conjugated secondary antibody (BA1054)
Secondary Incubation	Incubate at room temperature for 2 hours.
Detection	ProteinSimple FluorChem system (BIO-RAD, ChemiDocTM XRS+)
Results Summary	The human SH-SY5Y cell line was used for this experiment. The resulting GAPDH protein band appeared at approximately 36 kDa; the band was distinct and showed stable expression with no non-specific bands. These results further confirm the antibody's excellent recognition capability and high specificity, aligning with expectations.

SKU	A00227-1
Application	Western Blot
Sample	human OCI-LY1 cellls
Sample Processing Description	Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents	5% non-fat milk
Primary Antibody	GAPDH Antibody Picoband®
Primary Incubation	1:5000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.

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Free Secondary Antibody

Anti-GAPDH Antibody Picoband®

GAPDH antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Repeated Moderate and Severe Heat Exposure Has Divergent Effects on Skeletal Muscle Mitochondrial, Proteostatic and Neuromuscular Outcomes in Mice

Virtual screening and MD simulation-guided discovery of CDK12-IN-3, a potent ALK inhibitor suppressing neuroblastoma growth in vitro and in vivo

Development of Oncolytic Virus-Proteolysis-Targeting Chimera Conjugates for Tumor Immunotherapy

Electroacupuncture ameliorates disuse muscular atrophy and inhibits the NLRP3 inflammasome and SIRT1 through miR-133a in a mouse model

Transcobalamin receptor CD320, responsible for vitamin B12 cellular uptake, is present on the cell surface as a homo-oligomer

Targeting heparanase-mediated glycocalyx degradation as a novel strategy to inhibit EMT-driven pulmonary fibrosis

IGF2BP3 promotes epithelial ovarian cancer progression by regulating FASN expression

CBLC-mediated ubiquitination stabilizes METTL1, enhances N7-methylguanosine modification of ESRRA, and promotes the progression of endometrial cancer

Hederagenin Alleviates Ulcerative Colitis via Suppressing the Activation of EGFR/p38 MAPK Signaling

NOTCH4 downregulation repolarizes tumor-associated macrophages and enhances the sorafenib sensitivity of colorectal cancer cells

hucMSCs alleviate LPS-induced endothelial glycocalyx damage by secreting Cxcl12 to activate CXCR4

CSTA and DDX24: potential biomarkers regulating ferroptosis in sepsis and their diagnostic value

Astragaloside IV targets TUBB4B to inhibit proliferation and promote apoptosis of pituitary tumor cells via the STMN1/ERK pathway

Delphinidin, a dietary anthocyanidin, modulates PD-L1 and STING/TBK1 signaling and reprograms the immune microenvironment in triple-negative breast cancer

Skin bacteriota ameliorates androgenetic alopecia via harmonizing skin immuno inflammatory balance

Benzo[a]pyrene lowers the sperm quality in male rats by downregulating the expression of ZMYND15 and ZMYND10

PLGA nanoparticles encapsulating fibroblast growth factor 21 alleviate acetaminophen-induced acute liver injury

Occupational silica exposure drives systemic immune dysregulation and tumor microenvironment susceptibility: evidence from a real-world study

Overexpression of GMFB Predicts Poor Prognosis and Correlates With EMT-Mediated Progression in Gastric Cancer

Skullcapflavone II Suppresses Colorectal Cancer by Inhibiting the HIF-1α-Glycolysis Pathway

The SOX1:c.1036A > G:p.M346V variant act as an pathogenic gene in nasopharyngeal carcinoma

4EBP1 phosphorylation mediates TGF-β1–induced hepatic fibrosis and upregulates HIF-1α expression

Endothelial HMGB1-AIM2 axis worsens myocardial ischemia—reperfusion injury by regulating endothelial pyroptosis

Three-dimensional scaffold-free periodontal ligament stem cell pellets for alveolar ridge preservation: an in vitro and in vivo study

The effect of microRNA-145 on proliferation and apoptosis of cutaneous squamous cell carcinoma cells

Exercise Training Promotes Neural Remodeling and Vascular Regeneration in Cerebral Ischemic Rats Through the JNK/c-jun Signaling Pathway

Ellagic acid alleviates iron overload-induced liver damage by mitigating ferroptosis through modulation of the TGFβ/Smad signaling pathway

Identification of Sinigrin as Active Compound of Rape Pollen for Treating Benign Prostatic Hyperplasia Through PI3K/AKT/mTOR Axis

A promising approach to diabetic osteoporosis: oxymatrine’s effects on gut microbiota and osteoblasts

MiR-27b-3p Reduces the Efficacy of Propranolol in the Treatment of Infantile Hemangioma by Inhibiting the Expression of Apaf-1

Investigating the Mechanism of the Fuzheng Huayu Formula in Treating Cirrhosis through Network Pharmacology, Molecular Docking, and Experimental Verification

Downregulation of CPT2 promotes proliferation and migration through the TNFα/NF-κB pathway in cholangiocarcinoma

TDH-11 inhibits the proliferation and colonization of colorectal cancer by reducing the activity of HDAC

Shuangshi Tonglin Capsule Improves Prostate Fibrosis through Nrf2/TGF-β1 Signaling Pathways

Mechanistic Studies on the Role of IL-17/NLRP3 in Arsenic-Induced Activation of Hepatic Stellate Cells Through Hepatocyte Proptosis

LncRNA H19 promotes adipogenic differentiation disorder by sponging miR-130b-3p to upregulate PPARγ in steroid-induced osteonecrosis of the femoral head

A bifunctional antibody targeting PD-1 and TGF-β signaling has antitumor activity in combination with radiotherapy and attenuates radiation-induced lung injury

Cornus Officinalis Total Glycosides Alleviate Granulomatous Lobular Mastitis via the B7-CD28/CTLA-4 Costimulatory Pathway

Lentivirus-mediated RNA interference targeting HMGB1 modulates AQP1 to reduce pain induced by chronic compression of the dorsal root ganglia

Irisin alleviates the pyroptosis of β cells in T2DM by inhibiting NLRP3 inflammasome through regulating miR-19b-3p/SOCS3/STAT3 axis mediated autophagy

The role of AdipoQ on proliferation, apoptosis, and hormone Secretion in chicken primary adenohypophysis cells

The Cytotoxic Effects of Human Mesenchymal Stem Cells Induced by Uranium

Epimedium-Curculigo herb pair enhances bone repair with infected bone defects and regulates osteoblasts through LncRNA MALAT1/miR-34a-5p/SMAD2 axis

Mechanism underlying the role of integrin α3β1 in adhesive dysfunction between thyroid cells induced by diesel engine exhaust particles

Epigallocatechin-3-gallate Inhibits LPS/AβO-induced Neuroinflammation in BV2 Cells through Regulating the ROS/TXNIP/NLRP3 Pathway

MYH7 R453C induced cardiac remodelling via activating TGF-β/Smad2/3, ERK1/2 and Nox4/ROS/NF-κB signalling pathways

Astragaloside IV prevents enterovirus 71-induced pyroptosis through the TCF12-TXNIP-Keap1/Nrf2 axis

Role of transforming growth factor-β1 pathway in angiogenesis induced by chronic stress in colorectal cancer

Metabolomics combined with network pharmacology reveals a role for astragaloside IV in inhibiting enterovirus 71 replication via PI3K-AKT signaling

Loss of SIL1 Affects Actin Dynamics and Leads to Abnormal Neural Migration

SHARPIN contributes to sevoflurane-induced neonatal neurotoxicity through up-regulating HMGB1 to repress M2 like-macrophage polarization

Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer

Sodium arsenite impairs sperm quality via downregulating the ZMYND15 and ZMYND10

Enhanced therapeutic potential of Flotillins-modified MenSCs by improve the survival, proliferation and migration

Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability

Tamarix aphylla derived metabolites ameliorate indomethacin-induced gastric ulcers in rats by modulating the MAPK signaling pathway, alleviating oxidative stress and inflammation: In vivo study supported by pharmacological network analysis

Revealing the mechanism of ethyl acetate extracts of Semen Impatientis against prostate cancer based on network pharmacology and transcriptomics

BUB1 Promotes Gemcitabine Resistance in Pancreatic Cancer Cells by Inhibiting Ferroptosis

Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study

Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway

Multidimensional data analysis revealed thyroiditis-associated TCF19 SNP rs2073724 as a highly ranked protective variant in thyroid cancer

The Nuclear Localization Signal of Porcine Circovirus Type 4 Affects the Subcellular Localization of the Virus Capsid and the Production of Virus-like Particles

Pan-cancer analysis identified OAS1 as a potential prognostic biomarker for multiple tumor types

Gemcitabine and Pin1 siRNA co-delivery with fucoidan-coated nano-liposomes for therapy of pancreatic cancer

The effect of Par3 on the cellular junctions and biological functions of odontoblast-lineage cells

Dexmedetomidine Improves Anxiety-like Behaviors in Sleep-Deprived Mice by Inhibiting the p38/MSK1/NFκB Pathway and Reducing Inflammation and Oxidative Stress

Role and mechanism of Circ-PDE7B in the formation of keloid