Click image to see more details
Product Info Summary
| SKU: | M12995 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GCET2 Monoclonal Antibody
SKU/Catalog Number
M12995
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GCET2 Monoclonal Antibody catalog # M12995. Tested in IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GCET2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12995)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFHF-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GCET2 Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation.
Reactive Species
M12995 is reactive to GCSAM in Human
Observed Molecular Weight
21 kDa
Calculated molecular weight
21.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12995 is guaranteed for Flow Cytometry, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100-1:500
ICC/IF 1:100-1:500
FC 1:100
Positive Control
IHC: human bladder cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human bladder cancer, using GCET2 Antibody.
Specific Publications For Anti-GCET2 Monoclonal Antibody (M12995)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GCET2 Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-GCET2 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-GCET2 Monoclonal Antibody
Question
We are currently using anti-GCET2 Monoclonal antibody M12995 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The anti-GCET2 Monoclonal antibody (M12995) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-03
Question
Does anti-GCET2 Monoclonal antibody M12995 work for IHC with lymphoma?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
According to the expression profile of lymphoma, GCSAM is highly expressed in lymphoma. So, it is likely that anti-GCET2 Monoclonal antibody M12995 will work for IHC with lymphoma.
Boster Scientific Support
Answered: 2017-10-09
Question
I am interested in to test anti-GCET2 Monoclonal antibody M12995 on human lymphoma for research purposes, then I may be interested in using anti-GCET2 Monoclonal antibody M12995 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Collins
Verified customer
Asked: 2017-01-12
Answer
The products we sell, including anti-GCET2 Monoclonal antibody M12995, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-01-12
Question
Is this M12995 anti-GCET2 Monoclonal antibody reactive to the isotypes of GCSAM?
P. Edwards
Verified customer
Asked: 2014-12-30
Answer
The immunogen of M12995 anti-GCET2 Monoclonal antibody is A synthesized peptide derived from human GCET2 Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-12-30
Question
Will M12995 anti-GCET2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Patel
Verified customer
Asked: 2013-06-19
Answer
As indicated on the product datasheet, M12995 anti-GCET2 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-06-19
