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GFAP antibody (M00213-8) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 28 Publications

m00213 8 gfap primary antibodies if testing 4

IF analysis of Histone H3 and GFAP using anti-Histone H3 antibody (A12477-2) and anti-GFAP antibody (M00213-8).
Histone H3 and GFAP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Histone H3 antibody (A12477-2) and mouse anti-GFAP antibody (M00213-8) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127), Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-GFAP Antibody Picoband® (monoclonal, 3F2)

GFAP antibody

28 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Mouse	IF, IHC, WB

Tested Applications

GFAP antibody was positively detected by WB in:

rat brain tissue, mouse brain tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

GFAP antibody was positively detected by IHC in:

human glioma tissue, rat brain tissue, rat cerebral cortex tissue, rat hippocampus tissue, mouse hippocampus tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

GFAP antibody was positively detected by IF in:

rat brain tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Rat

Tested

Mouse

Tested

Recommend

Human

Tested

Recommend

Monkey

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Rabbit

Predicted

Product Information

Product Name

Anti-GFAP Antibody Picoband® (monoclonal, 3F2)
View all GFAP Antibodies

Form

Lyophilized

Uniprot

P14136

Gene ID

2670

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

3F2

Isotype

Mouse IgG1

Immunogen

E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP.

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Observed Molecular Weight

50 kDa

Calculated Molecular Weight

49.9 kDa

Background of GFAP

Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, and ependymal cells. It is mapped to 17q21.31. GFAP is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells. This gene has been shown to play a role in mitosis by adjusting the filament network present in the cell. GFAP is necessary for many critical roles in the CNS. What’s more, GFAP also plays a role in astrocyte-neuron interactions as well as cell-cell communication.

Cite This Product

Anti-GFAP Antibody Picoband® (monoclonal, 3F2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00213-8)

Protocols

Western Blot protocol for GFAP antibody

Review Protocol

Immunohistochemistry (IHC) protocol for GFAP antibody

Review Protocol

Immunofluorescence (IF) protocol for GFAP antibody

Review Protocol

Publications

Tauroursodeoxycholic acid regulates macrophage/monocyte distribution and improves spinal microenvironment to promote nerve regeneration through inhibiting NF-κB signaling pathway in spinal cord injury

Published: 2025-04-10

Journal: Frontiers in Pharmacology

Application: IF

Impact Factor: 4.80

Species: Mouse

PMID: 40276612

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Free Secondary Antibody

Anti-GFAP Antibody Picoband® (monoclonal, 3F2)

GFAP antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Tauroursodeoxycholic acid regulates macrophage/monocyte distribution and improves spinal microenvironment to promote nerve regeneration through inhibiting NF-κB signaling pathway in spinal cord injury

Chitosan-Modified Hydrogel Microsphere Encapsulating Zinc-Doped Bioactive Glasses for Spinal Cord Injury Repair by Suppressing Inflammation and Promoting Angiogenesis

The impact of ICOSL/ICOS pathway-regulated long non-coding RNAs on liver fibrosis in mice infected with Schistosoma japonicum

A neurotherapeutic approach with Lacticaseibacillus rhamnosus E9 on gut microbiota and intestinal barrier in MPTP-induced mouse model of Parkinson’s disease

Transplantation of Wnt5a-modified Bone Marrow Mesenchymal Stem Cells Promotes Recovery After Spinal Cord Injury via the PI3K/AKT Pathway

Menthol-Modified Quercetin Liposomes with Brain-Targeting Function for the Treatment of Senescent Alzheimer’s Disease

Spinal HMGB1 participates in the early stages of paclitaxel-induced neuropathic pain via microglial TLR4 and RAGE activation

SerpinA3N attenuates ischemic stroke injury by reducing apoptosis and neuroinflammation

Resistance Exercise and Whey Protein Supplementation Reduce Mechanical Allodynia and Spinal Microglia Activation After Acute Muscle Trauma in Rats

NDGA-P21, a novel derivative of nordihydroguaiaretic acid, inhibits glioma cell proliferation and stemness

3D bioprinted glioma stem cells for brain tumor model and applications of drug susceptibility

Effect of Corilagin on the Proliferation and NF-κB in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells

Lentiviral Vector-Induced Overexpression of RGMa in the Hippocampus Suppresses Seizures and Mossy Fiber Sprouting

Elevated Expression of the Delta-Subunit of Epithelial Sodium Channel in Temporal Lobe Epilepsy Patients and Rat Model

β-elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells

Decreased expression of Gab2 in patients with temporal lobe epilepsy and pilocarpine-induced rat model

Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection

Expression pattern of sorting nexin 25 in temporal lobe epilepsy: A study on patients and pilocarpine-induced rats

ADAM17 promotes U87 glioblastoma stem cell migration and invasion

Decreased expression of Ras-GRF1 in the brain tissue of the intractable epilepsy patients and experimental rats

Protective effects of cationic bovine serum albumin-conjugated PEGylated tanshinone IIA nanoparticles on cerebral ischemia

Down-Regulation of CRMP-1 in Patients with Epilepsy and a Rat Model

Increased expression of calponin-3 in epileptic patients and experimental rats

Transplantation of neural stem cells enhances expression of synaptic protein and promotes functional recovery in a rat model of traumatic brain injury

A rat model of mild cognitive impairment associated with vascular factor

Induced Multilineage Differentiation of Chicken Embryonic Germ Cells via Embryoid Body Formation

Clinical, histopathological and genetic studies in a family with fatal familial insomnia

Brain injury due to acute organophosphate poisoning: Magnetic resonance imaging manifestation and pathological characteristics

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