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Product Info Summary
| SKU: | M00213-8 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-GFAP Antibody Picoband™ (monoclonal, 3F2)
SKU/Catalog Number
M00213-8
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GFAP Antibody Picoband™ (monoclonal, 3F2) catalog # M00213-8. Tested in IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GFAP Antibody Picoband™ (monoclonal, 3F2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00213-8)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
3F2
Isotype
IgG1
Immunogen
E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross Reactivity
No cross reactivity with other proteins.
Reactive Species
M00213-8 is reactive to GFAP in Human, Mouse, Rat
Applications
M00213-8 is guaranteed for IHC-P, WB Boster Guarantee
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GFAP using anti-GFAP antibody (M00213-8).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GFAP antigen affinity purified monoclonal antibody (Catalog # M00213-8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GFAP at approximately 50KD. The expected band size for GFAP is at 50KD.
Click image to see more details
Figure 2. IHC analysis of GFAP using anti-GFAP antibody (M00213-8).
GFAP was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GFAP Antibody (M00213-8) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of GFAP using anti-GFAP antibody (M00213-8).
GFAP was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GFAP Antibody (M00213-8) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GFAP (Source: Uniprot.Org, NCBI)
Uniprot ID
P14136
Gene ID
2670
Gene Name
GFAP
Full Name
Glial fibrillary acidic protein
Weight
49.88kDa
Superfamily
intermediate filament family
Alternative Names
FLJ45472; GFAP astrocytes; GFAP immunohistochemistry; GFAP mouse; GFAP rabbit; GFAP stain; GFAP; glial fibrillary acidic protein GFAP ALXDRD glial fibrillary acidic protein glial fibrillary acidic protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GFAP, check out the GFAP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GFAP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-GFAP Antibody Picoband™ (monoclonal, 3F2) (M00213-8)
M00213-8 has been cited in 29 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu Y,Li L,Qiu M,Tan L,Zhang M,Li J,Zhu H,Jiang S,Su X,Li A.Renal and cerebral RAS interaction contributes to diabetic kidney disease.Am J Transl Res.2019 May 15;11(5):2925-2939.PMID:31217864;PMCID:PMC6556645.
Species: Rat
M00213-8 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:NA
Ren Y,Jiang J,Jiang W,Zhou X,Lu W,Wang J,Luo Y.Spata2 Knockdown Exacerbates Brain Inflammation via NF-κB/P38MAPK Signaling and NLRP3 Inflammasome Activation in Cerebral Ischemia/Reperfusion Rats.Neurochem Res.2021 Jun 1.doi:10.1007/s11064-021-03360-8.Epub ahead of print.PMID:34075523.
Species: Rat
M00213-8 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Liu J,Zhang N,Zhang M,Yin H,Zhang X,Wang X,Wang X,Zhao Y.N-acetylserotonin alleviated the expression of interleukin-1β in retinal ischemia-reperfusion rats via the TLR4/NF-κB/NLRP3 pathway.Exp Eye Res.2021 May 14:108595.doi:10.1016/j.exer.2021.108595.Epub ahead of print.PMID:34000276.
Species: Rat
M00213-8 usage in article: APP:IF, SAMPLE:RETINAL TISSUE, DILUTION:1:100
Xiang Z,Jiang X,Ji R,Yuan H.Enhanced expression of P2X4 purinoceptors in pyramidal neurons of the rat hippocampal CA1 region may be involved ischemia-reperfusion injury.Purinergic Signal.2021 May 9.doi:10.1007/s11302-021-09780-z.Epub ahead of print.PMID:33966147.
Species: Rat
M00213-8 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:400
Zhu X,Yao Y, Yang J,Zhang C,Li X,Zhang A,Liu X,Zhang C,Gan G.ADAM10 suppresses demyelination and reduces seizure susceptibility in cuprizone-induced demyelination model.Free Radic Biol Med.2021 May 6:S0891-584 9(21)00282-3.doi:10.1016/j.freeradbiomed.2021.05.001.Epub ahead of print.PMID:33965566.
Species: Mouse
M00213-8 usage in article: APP:ICC, SAMPLE:CORPUS CALLOSUM TISSUE, DILUTION:1:100
Xiang S,Zhao D,Hao H,Wang XU,Li L,Yang T.α-Helical protein absorption at post-traumatic epileptic foci monitored by Fourier transform infrared mapping.J Biosci.2020;45:55.PMID:32345781.
Species: Rat
M00213-8 usage in article: APP:IHC, SAMPLE:FRONTAL BRAIN LOBE, DILUTION:1:100
Xu H,Wang Y,Luo Y.OTULIN is a new target of EA treatment in the alleviation of brain injury and glial cell activation via suppression of the NF-κB signalling pathway in acute ischaemic stroke rats.Mol Med.2021 Apr 9;27(1):37.doi:10.1186/s10020-021-00297-0.PMID:33836646;PMCID:PMC8035756.
Species: Rat
M00213-8 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION: NA
Moraes TR,Elisei LS,Malta IH,Galdino G.Participation of CXCL1 in the glial cells during neuropathic pain.Eur J Pharmacol.2020 May 15;875:173039.doi:10.1016/j.ejphar.2020.173039.Epub 2020 Feb 28.PMID:32119843.
Species: Rat
M00213-8 usage in article: APP:IF, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:100
Zhang Y,Zhang M,Zhu W,Pan X,Wang Q,Gao X,Wang C,Zhang X,Liu Y,Li S,Sun H.Role of Elevated Thrombospondin-1 in Kainic Acid-Induced Status Epilepticus.Neurosci Bull.2020 Mar;36(3):263-276.doi:10.1007/s12264-019-00437-x.Epub 2019 Oct 29.PMID:31664678;PMCID:P
Species: Rat
M00213-8 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS AND CORTEX, DILUTION:1:1000
Deng J,Xu T,Yang J,Zhang KM,Li Q,Yu XY,Li R,Fu J,Jiang Q,Ma JX,Chen YM.Sema7A, a brain immune regulator, regulates seizure activity in PTZ-kindled epileptic rats.CNS Neurosci Ther.2020 Jan;26(1):101-116.doi:10.1111/cns.13181.Epub 2019 Jun 9.PMID:31179640;
Species: Human,Rat
M00213-8 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Chen X,Zhang L,Hua F,Zhuang Y,Liu H,Wang S.EphA4 Obstructs Spinal Cord Neuron Regeneration by Promoting Excessive Activation of Astrocytes.Cell Mol Neurobiol.2021 Feb 17.doi:10.1007/s10571-021-01046-x.Epub ahead of print.PMID:33595805.
Species: Mouse
M00213-8 usage in article: APP:IF, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:500
Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Yang H,Zhang C.Expression and cellular distribution of FGF13 in cortical tubers of the tuberous sclerosis complex.Neurosci Lett. 2021 Feb 11:135714.doi:10.1016/j.neulet.2021.135714.Epub ahead of print.PMID:33582188.
Species: Human
M00213-8 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:50
Hongbei Xu,You Wang,Yong Luo et al.OTULIN is a New Target for EA Treatment in Alleviating Brain Injury and The Activation of Glial Cells By Depressing NF-κB Signalling Pathway in Acute Ischaemic Stroke Rats,28 January 2021,PREPRINT (Version 1) available a
Species: Rat
M00213-8 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:NA
Zhang LY,Jin QQ,Hölscher C,Li L.Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model. Neural Regen Res 2021;16:1660-70
Species: Human,Rat
M00213-8 usage in article: APP:IF, SAMPLE:SN TISSUE, DILUTION:1:100
Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
M00213-8 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020, PREPRINT (Version 1) available at Research Square [https:/
Species: Mouse
M00213-8 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Ahmed S. Ahmed,JAK-1/STAT-3 pathway mediated role in aging cerebellar cortex degenerative changes of albino wistar rats, Translational Research in Anatomy, 2020,100089,ISSN 2214-854X, https://doi.org/10. 1016/j.tria.2020.100089.
Species: Rat
M00213-8 usage in article: APP:IHC, SAMPLE:CEREBELLAR CORTEX, DILUTION:1:20
Zhou Feng,Shengyan Liu,Qianwei Chen,Qiang Tan,Jishu Xian,Hua Feng,Zhi Chen,Gang Li,uPA alleviates kaolin-induced hydrocephalus by promoting the release and activation of hepatocyte growth factor in rats, Neuroscience Letters,Volume 731,2020,135011,ISSN 03
Species: Rat
M00213-8 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Jian Wang,Xiaolin Li,Yu Song,Qiangfei Su,Xiakeerzhati Xiaohalati,Wen Yang,Luming Xu,Bo Cai,Guobin Wang,Zheng Wang,Lin Wang,Injectable silk sericin scaffolds with programmable shape-memory property and neuro-differentiation-promoting activity for individua
Species: Silkworm,Mouse
M00213-8 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020,PREPRINT (Version 1)available at Research Square [+https://
Species: Mouse
M00213-8 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Luo Z,Wang J,Tang S,Zheng Y,Zhou X,Tian F,Xu Z.Dynamic-related protein 1 inhibitor eases epileptic seizures and can regulate equilibrative nucleoside transporter 1 expression.BMC Neurol.2020 Sep 22;20(1):353.doi:10.1186/s12883-020-01921-y.PMID:32962663;PM
Species: Rat
M00213-8 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Wang X,Tong Y,Zhang J,Khan N,Zhang K,Bai H,Zhang Q,Chen Y.Neuroinflammation changes with periodontal inflammation status during periodontitis in wild-type mice.Oral Dis.2020 Aug 20.doi:10.1111/odi.13618.Epub ahead of print.PMID:32815656.
Species: Mouse
M00213-8 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:50
Wang X,Zhou W,Li X,Ren J,Ji G,Du J,Tian W,Liu Q,Hao A. Graphene oxide suppresses the growth and malignancy of glioblastoma stem cell-like spheroids via epigenetic mechanisms. J Transl Med.2020 May 14;18(1):200. doi:10.1186/s12967-020-02359-z.PMID:32410622
Species: Human
M00213-8 usage in article: APP:WB, SAMPLE:GSCS, DILUTION:1:1000
Li R,Wen Y,Wu B,He M,Zhang P,Zhang Q,Chen Y.MicroRNA-25-3p suppresses epileptiform discharges through inhibiting oxidative stress and apoptosis via targeting OXSR1 in neurons.Biochem Biophys Res Commun. 2020 Mar 19;523(4):859-866.doi:10.1016/j.bbrc.2020.
Species: Rat
M00213-8 usage in article: APP:IHC, SAMPLE:HIPPOCAMPUL NEURONS, DILUTION:NA
Deng Z,Wei Y,Yao Y,Gao S,Wang X.Let-7f promotes the differentiation of neural stem cells in rats.Am J Transl Res.2020 Sep 15;12(9):5752-5761.PMID:33042454;PMCID:PMC7540113.
Species: Rat
M00213-8 usage in article: APP:IF, SAMPLE:NSCS, DILUTION:1:100
Li X,Shi MQ,Chen C,Du JR.Phthalide derivative CD21 ameliorates ischemic brain injury in a mouse model of global cerebral ischemia: involvement of inhibition of NLRP3.Int Immunopharmacol.2020 Sep;86:106714.doi: 10.1016/j.intimp.2020.106714.Epub 2020 Jun 24
Species: Mouse
M00213-8 usage in article: APP:IF, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:100
Xue NY,Ge DY,Dong RJ,Kim HH,Ren XJ,Tu Y.Effect of electroacupuncture on glial fibrillary acidic protein and nerve growth factor in the hippocampus of rats with hyperlipidemia and middle cerebral artery thrombus. Neural Regen Res.2021 Jan;16(1):137-142.doi
Species: Rat
M00213-8 usage in article: APP:IHC, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:100
Zhu X,Yao Y,Yang J,Zhengxie J,Li X,Hu S,Zhang A,Dong J,Zhang C,Gan G.COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice. Int Immunopharmacol. 2020 Oct;87:106801.doi: 10.1016/j.inti
Species: Mouse
M00213-8 usage in article: APP:ICC, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Zhou Z,Hou J,Mo Y,Ren M,Yang G,Qu Z,Hu Y.Geniposidic acid ameliorates spatial learning and memory deficits and alleviates neuroinflammation via inhibiting HMGB-1 and downregulating TLR4/2 signaling pathway in APP/PS1 mice.Eur J Pharmacol.2020 Feb 15;869:1
Species: Mouse
M00213-8 usage in article: APP:IHC, SAMPLE:BRAIN SECTION, DILUTION:1:200
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-GFAP Antibody Picoband™ (monoclonal, 3F2)
Question
We are currently using anti-GFAP in antibody (monoclonal, 3F2) M00213-8 for rat tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The anti-GFAP in antibody (monoclonal, 3F2) (M00213-8) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-04
Question
My team were content with the WB result of your anti-GFAP in antibody (monoclonal, 3F2). However we have observed positive staining in blood cytoplasm. using this antibody. Is that expected? Could you tell me where is GFAP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
From what I have seen in literature, blood does express GFAP. Generally GFAP expresses in cytoplasm. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2019-11-28
Question
We have tried in the past anti-GFAP in antibody (monoclonal, 3F2) for IHC-P on dorsal motor nucleus of vagus nerve in a previous project. I am using rat, and We are going to use the antibody for WB next. Our lab want to know about examining dorsal motor nucleus of vagus nerve as well as brain thalamus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
B. Krishna
Verified customer
Asked: 2017-02-23
Answer
I viewed the website and datasheets of our anti-GFAP in antibody (monoclonal, 3F2) and I see that M00213-8 has been tested on rat in both IHC-P and WB. Thus M00213-8 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-02-23
Question
We have seen staining in rat fetal brain. Any tips? Is anti-GFAP in antibody (monoclonal, 3F2) supposed to stain fetal brain positively?
E. Wu
Verified customer
Asked: 2016-07-27
Answer
According to literature fetal brain does express GFAP. According to Uniprot.org, GFAP is expressed in dorsal motor nucleus of vagus nerve, brain thalamus, brain, kidney, fetal brain cortex, fetal brain, blood, among other tissues. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2016-07-27
