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Product Info Summary
| SKU: | M03394-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-NG2 Purified CSPG4 Monoclonal Antibody
SKU/Catalog Number
M03394-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NG2 Purified CSPG4 Monoclonal Antibody (Catalog# M03394-1). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-NG2 Purified CSPG4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03394-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
7.1
Isotype
Mouse IgG1
Immunogen
Human bone marrow stromal cells infected with SV-40. The mouse monoclonal antibody 7.1 recognizes an extracellular epitope of NG2, the melanoma-associated chondroitin sulfate proteoglycan 4 of Mw approximately 220-300 kDa.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M03394-1 is reactive to CSPG4 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
250.5 kDa
Background of CSPG4
NG2 / chondroitin sulfate proteoglycan 4 is expressed on glial cell populations, but not on normal hepatopoietic cells. It is an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells, where it plays role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes, and supports signaling pathways important for tumor invasion and growth. NG2 also serves as an AML blast tumor marker associated with poor prognosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03394-1 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Separation of SK-MEL-30 cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human NG2 (7.1) purified antibody (concentration in sample 0.6 µg/ml) GAM APC.
Specific Publications For Anti-NG2 Purified CSPG4 Monoclonal Antibody (M03394-1)
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