Anti-GFAP Rabbit Monoclonal Antibody

GFAP antibody

Boster Bio Anti-GFAP Rabbit Monoclonal Antibody catalog # M00213-1. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: M00213-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

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Product Name

Anti-GFAP Rabbit Monoclonal Antibody

View all GFAP Antibodies

SKU/Catalog Number

M00213-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-GFAP Rabbit Monoclonal Antibody catalog # M00213-1. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GFAP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00213-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DBI-7

Immunogen

A synthesized peptide derived from human GFAP

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00213-1 is reactive to GFAP in Human, Mouse, Rat

Applications

M00213-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:10000-1:50000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30

Validation Images & Assay Conditions

Gene/Protein Information For GFAP (Source: Uniprot.org, NCBI)

Uniprot ID

P14136

Gene ID

2670

Gene Name

GFAP

Full Name

Glial fibrillary acidic protein

Weight

49.88kDa

Superfamily

intermediate filament family

Alternative Names

FLJ45472; GFAP astrocytes; GFAP immunohistochemistry; GFAP mouse; GFAP rabbit; GFAP stain; GFAP; glial fibrillary acidic protein GFAP ALXDRD glial fibrillary acidic protein glial fibrillary acidic protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GFAP, check out the GFAP Infographic

GFAP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GFAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

M00213-1 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Deng J,Xu T,Yang J,Zhang KM,Li Q,Yu XY,Li R,Fu J,Jiang Q,Ma JX,Chen YM.Sema7A, a brain immune regulator, regulates seizure activity in PTZ-kindled epileptic rats.CNS Neurosci Ther.2020 Jan;26(1):101-116.doi:10.1111/cns.13181.Epub 2019 Jun 9.PMID:31179640;
Species: Human,Rat
M00213-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100

Chen X,Zhang L,Hua F,Zhuang Y,Liu H,Wang S.EphA4 Obstructs Spinal Cord Neuron Regeneration by Promoting Excessive Activation of Astrocytes.Cell Mol Neurobiol.2021 Feb 17.doi:10.1007/s10571-021-01046-x.Epub ahead of print.PMID:33595805.
Species: Mouse
M00213-1 usage in article: APP:IF, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:500

Zhang LY,Jin QQ,Hölscher C,Li L.Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model. Neural Regen Res 2021;16:1660-70
Species: Human,Rat
M00213-1 usage in article: APP:IF, SAMPLE:SN TISSUE, DILUTION:1:100

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
M00213-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020, PREPRINT (Version 1) available at Research Square [https:/
Species: Mouse
M00213-1 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Ahmed S. Ahmed,JAK-1/STAT-3 pathway mediated role in aging cerebellar cortex degenerative changes of albino wistar rats, Translational Research in Anatomy, 2020,100089,ISSN 2214-854X, https://doi.org/10. 1016/j.tria.2020.100089.
Species: Rat
M00213-1 usage in article: APP:IHC, SAMPLE:CEREBELLAR CORTEX, DILUTION:1:20

Zhou Feng,Shengyan Liu,Qianwei Chen,Qiang Tan,Jishu Xian,Hua Feng,Zhi Chen,Gang Li,uPA alleviates kaolin-induced hydrocephalus by promoting the release and activation of hepatocyte growth factor in rats, Neuroscience Letters,Volume 731,2020,135011,ISSN 03
Species: Rat

Jian Wang,Xiaolin Li,Yu Song,Qiangfei Su,Xiakeerzhati Xiaohalati,Wen Yang,Luming Xu,Bo Cai,Guobin Wang,Zheng Wang,Lin Wang,Injectable silk sericin scaffolds with programmable shape-memory property and neuro-differentiation-promoting activity for individua
Species: Silkworm,Mouse
M00213-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100

Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020,PREPRINT (Version 1)available at Research Square [+https://
Species: Mouse
M00213-1 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Luo Z,Wang J,Tang S,Zheng Y,Zhou X,Tian F,Xu Z.Dynamic-related protein 1 inhibitor eases epileptic seizures and can regulate equilibrative nucleoside transporter 1 expression.BMC Neurol.2020 Sep 22;20(1):353.doi:10.1186/s12883-020-01921-y.PMID:32962663;PM
Species: Rat
M00213-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200

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18 Customer Q&As for Anti-GFAP Rabbit Monoclonal Antibody

Question

Is there a BSA free version of anti-GFAP Rabbit Monoclonal antibody M00213-1 available?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GFAP Rabbit Monoclonal antibody M00213-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-09

Question

We have seen staining in rat brain thalamus. Are there any suggestions? Is anti-GFAP Rabbit Monoclonal antibody supposed to stain brain thalamus positively?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

From literature brain thalamus does express GFAP. From Uniprot.org, GFAP is expressed in dorsal motor nucleus of vagus nerve, brain thalamus, brain, kidney, fetal brain cortex, fetal brain, blood, among other tissues. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2020-01-09

Question

I see that the anti-GFAP Rabbit Monoclonal antibody M00213-1 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-23

Question

I am interested in using your anti-GFAP Rabbit Monoclonal antibody for nuclear signaling by erbb4 studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

Thanks for your inquiry. This M00213-1 anti-GFAP Rabbit Monoclonal antibody is validated on rat brain tissue, mouse brain. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-29

Question

We ordered your anti-GFAP Rabbit Monoclonal antibody for IF on blood in the past. I am using human, and We want to use the antibody for WB next. My lab would like examining blood as well as kidney in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

I took a look at the website and datasheets of our anti-GFAP Rabbit Monoclonal antibody and I see that M00213-1 has been validated on human in both IF and WB. Thus M00213-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-21

Question

We are currently using anti-GFAP Rabbit Monoclonal antibody M00213-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

The anti-GFAP Rabbit Monoclonal antibody (M00213-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-15

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-GFAP Rabbit Monoclonal antibody M00213-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-15

Question

Has M00213-1 been tested for Flow Cytometry application?

Verified customer

Asked: 2019-07-04

Answer

The Anti-GFAP Rabbit Monoclonal Antibody (M00213-1) can't work for Flow Cytometry application.

Boster Scientific Support

Answered: 2019-07-05

Question

Can FITC or Alexfluor 488 secondary antibodies work for M00213-1?

Verified customer

Asked: 2019-07-04

Answer

FITC or Alexfluor 488 secondary antibodies could work for the Anti-GFAP Rabbit Monoclonal Antibody (M00213-1).

Boster Scientific Support

Answered: 2019-07-04

Question

Does anti-GFAP Rabbit Monoclonal antibody M00213-1 work for IF with kidney?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

According to the expression profile of kidney, GFAP is highly expressed in kidney. So, it is likely that anti-GFAP Rabbit Monoclonal antibody M00213-1 will work for IF with kidney.

Boster Scientific Support

Answered: 2019-06-21

Question

Will M00213-1 anti-GFAP Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

As indicated on the product datasheet, M00213-1 anti-GFAP Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-05

Question

I have a question about product M00213-1, anti-GFAP Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00213-1 anti-GFAP Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-28

Question

Is a blocking peptide available for product anti-GFAP Rabbit Monoclonal antibody (M00213-1)?

Verified Customer

Verified customer

Asked: 2018-12-14

Answer

We do provide the blocking peptide for product anti-GFAP Rabbit Monoclonal antibody (M00213-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-12-14

Question

I was wanting to use your anti-GFAP Rabbit Monoclonal antibody for IF for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?

Verified Customer

Verified customer

Asked: 2017-07-25

Answer

You can see on the product datasheet, M00213-1 anti-GFAP Rabbit Monoclonal antibody has been validated for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-07-25

Question

Here is the WB image, lot number and protocol we used for kidney using anti-GFAP Rabbit Monoclonal antibody M00213-1. Please let me know if you require anything else.

D. Carter

Verified customer

Asked: 2014-10-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-10-28

Question

Is this M00213-1 anti-GFAP Rabbit Monoclonal antibody reactive to the isotypes of GFAP?

D. Rodriguez

Verified customer

Asked: 2014-08-26

Answer

The immunogen of M00213-1 anti-GFAP Rabbit Monoclonal antibody is A synthesized peptide derived from human GFAP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-08-26

Question

My team were satisfied with the WB result of your anti-GFAP Rabbit Monoclonal antibody. However we have observed positive staining in brain cytoplasm. using this antibody. Is that expected? Could you tell me where is GFAP supposed to be expressed?

Z. Roberts

Verified customer

Asked: 2013-03-27

Answer

Based on literature, brain does express GFAP. Generally GFAP expresses in cytoplasm. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2013-03-27

Question

My lab would like to test anti-GFAP Rabbit Monoclonal antibody M00213-1 on human kidney for research purposes, then I may be interested in using anti-GFAP Rabbit Monoclonal antibody M00213-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

A. Parker

Verified customer

Asked: 2013-02-25

Answer

The products we sell, including anti-GFAP Rabbit Monoclonal antibody M00213-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-02-25

Size

Total: $315

SKU:M00213-1

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M00213-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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