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- Table of Contents
GFAP antibody (M00213-1) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 25 Publications
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Immunofluorescent analysis of SH-SY5Y cells, using GFAP Antibody .
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Anti-GFAP Rabbit Monoclonal Antibody
GFAP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
GFAP antibody was positively detected by WB in:
human U251 whole cell, rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GFAP antibody was positively detected by IHC in:
human glioma tissue, human glioma tissue, mouse brain tissue, mouse brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GFAP antibody was positively detected by ICC/IF in:
SH-SY5Y cell
Recommend Dilution
Application
Dilution
Species
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-GFAP Rabbit Monoclonal Antibody
View all GFAP Antibodies
View all GFAP Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DBI-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GFAP
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
45 kDa
Calculated Molecular Weight
49.9 kDa
Cite This Product
Anti-GFAP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00213-1)
Protocols
Western Blot protocol for GFAP antibody
Immunohistochemistry (IHC) protocol for GFAP antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for GFAP antibody
Publications
Application:
Species:
Melatonin Promotes Cerebral Angiogenesis in Ischemic Mice via BMP6/Smad1/5/9 Pathway
Published: 2025-04-25
Journal: MOLECULAR NEUROBIOLOGY
Application: IF
Impact Factor: 4.30
Species: Mouse
PMID: 40274709
Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model
Published: 2025-01-23
Journal: Animal Models and Experimental Medicine
Application: WB,IF
Impact Factor: 3.40
Species: Mouse
PMID: 39853719
Chitosan-Modified Hydrogel Microsphere Encapsulating Zinc-Doped Bioactive Glasses for Spinal Cord Injury Repair by Suppressing Inflammation and Promoting Angiogenesis
Published: 2024-11-09
Journal: Advanced Healthcare Materials
Application: IF
Impact Factor: 10.00
Species: Mouse
PMID: 39520381
The impact of ICOSL/ICOS pathway-regulated long non-coding RNAs on liver fibrosis in mice infected with Schistosoma japonicum
Published: 2024-07-23
Journal: Parasites & Vectors
Application: IF
Impact Factor: 3.00
Species: Mouse
PMID: 39044218
Pathological polarizations from microglia to astrocyte contributes to spatial memory deficit in methamphetamine abstinence mice
Published: 2024-07-09
Journal: CEREBRAL CORTEX
Application: IF
Impact Factor: 2.90
Species: Mouse
PMID: 38981852
A neurotherapeutic approach with Lacticaseibacillus rhamnosus E9 on gut microbiota and intestinal barrier in MPTP-induced mouse model of Parkinson’s disease
Published: 2024-07-04
Journal: Scientific Reports
Application: IHC
Impact Factor: 3.80
Species: Mouse,Human
PMID: 38965287
Menthol-Modified Quercetin Liposomes with Brain-Targeting Function for the Treatment of Senescent Alzheimer’s Disease
Published: 2024-05-23
Journal: ACS Chemical Neuroscience
Application: IHC
Impact Factor: 4.10
Species: Mouse
PMID: 38780450
STZ-induced diabetes exacerbates neurons ferroptosis after ischemic stroke by upregulating LCN2 in neutrophils
Published: 2024-04-24
Journal: EXPERIMENTAL NEUROLOGY
Application: IF
Impact Factor: 4.60
Species: Mouse
PMID: 38670252
Salidroside promotes angiogenesis after cerebral ischemia in mice through Shh signaling pathway
Published: 2024-04-20
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: IF
Impact Factor: 6.90
Species: Mouse
PMID: 38643543
Sevoflurane Alters Serum Metabolites in Elders and Aging Mice and Increases Inflammation in Hippocampus
Published: 2024-02-23
Journal: Journal of Inflammation Research
Application: IHC
Impact Factor: 4.20
Species: Mouse
PMID: 38415263
Satellite glial cell-secreted exosomes after in-vitro oxaliplatin treatment presents a pro-nociceptive effect for dorsal root ganglion neurons and induce mechanical hypersensitivity in naïve mice
Published: 2023-07-18
Journal: MOLECULAR AND CELLULAR NEUROSCIENCE
Application: ICC
Impact Factor: 3.50
Species: Mouse
PMID: 37467904
Spatiotemporal evolution of pyroptosis and canonical inflammasome pathway in hSOD1G93A ALS mouse model
Published: 2022-08-09
Journal: BMC NEUROSCIENCE
Application: IF
Impact Factor: 3.26
Species: Mouse
PMID: 35945502
Negative effects of brain regulatory T cells depletion on epilepsy
Published: 2022-08-02
Journal: PROGRESS IN NEUROBIOLOGY
Application: IHC
Impact Factor: 10.89
Species: Human,Mouse
PMID: 35931355
Neuroprotective effects of leptin on cerebral ischemia through JAK2/STAT3/PGC-1-mediated mitochondrial function modulation
Published: 2020-01-11
Journal: BRAIN RESEARCH BULLETIN
Application: IF
Impact Factor: 3.37
Species: Mouse
PMID: 31935431
Geniposidic acid ameliorates spatial learning and memory deficits and alleviates neuroinflammation via inhibiting HMGB-1 and downregulating TLR4/2 signaling pathway in APP/PS1 mice
Published: 2019-12-12
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: IHC
Impact Factor: 3.17
Species: Mouse
PMID: 31837991
Activation of leukocyte immunoglobulin-like receptor B2 signaling pathway in cortical lesions of pediatric patients with focal cortical dysplasia type IIb and tuberous sclerosis complex
Published: 2019-09-05
Journal: BRAIN & DEVELOPMENT
Application: IF
Impact Factor: 1.76
Species: Human
PMID: 31495513
Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment
Published: 2019-03-01
Journal: Neural Regeneration Research
Application: IF
Impact Factor: 2.47
Species: Mouse
PMID: 30539813
Melatonin Enhances Proliferation and Modulates Differentiation of Neural Stem Cells Via Autophagy in Hyperglycemia
Published: 2019-01-14
Journal: STEM CELLS
Application: ICC
Impact Factor: 5.61
Species: Mouse
PMID: 30644149
Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress
Published: 2018-04-04
Journal: Gastroenterology Research and Practice
Application: ICC
Impact Factor: 1.86
Species: Rat
PMID: 29849603
Evaluation of Connexin 43 Redistribution and Endocytosis in Astrocytes Subjected to Ischemia/Reperfusion or Oxygen-Glucose Deprivation and Reoxygenation
Published: 2017-03-23
Journal: Biomed Research International
Application: IF
Impact Factor: 2.48
Species: Rat
PMID: 28424784
Inhibition of Calcineurin A by FK506 Suppresses Seizures and Reduces the Expression of GluN2B in Membrane Fraction
Published: 2017-03-15
Journal: NEUROCHEMICAL RESEARCH
Application: IF
Impact Factor: 2.58
Species: Human,Rat
PMID: 28299629
Effect of synaptic adhesion-like molecule 3 on epileptic seizures: Evidence from animal models
Published: 2017-03-14
Journal: EPILEPSY & BEHAVIOR
Application: IF
Impact Factor: 2.63
Species: Rat
PMID: 28222338
Activation of VEGF/Flk-1-ERK Pathway Induced Blood–Brain Barrier Injury After Microwave Exposure
Published: 2014-09-09
Journal: MOLECULAR NEUROBIOLOGY
Application: ICC
Impact Factor: 5.29
Species: Rat
PMID: 25195697
Protective effects of cationic bovine serum albumin-conjugated PEGylated tanshinone IIA nanoparticles on cerebral ischemia
Published: 2012-10-27
Journal: BIOMATERIALS
Impact Factor: 7.40
PMID: 23111336
Structural and functional improvement of injured brain after severe acute carbon monoxide poisoning by stem cell–based therapy in rats*
Published: 2009-04-01
Journal: CRITICAL CARE MEDICINE
Application: ICC,IF,IHC
Impact Factor: 6.59
Species: Rat
PMID: 19242325
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Product Promise
We promise Anti-GFAP Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00213-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026