Anti-GFAP Rabbit Monoclonal Antibody

Western blot analysis of GFAP using anti-GFAP antibody (M00213-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFAP antigen affinity purified polyclonal antibody (Catalog # M00213-1) at 1:10000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFAP at approximately 45 kDa. The expected band size for GFAP is at 50 kDa.

IHC analysis of GFAP using anti-GFAP antibody (M00213-1).
GFAP was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GFAP Antibody (M00213-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of GFAP using anti-GFAP antibody (M00213-1).
GFAP was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GFAP Antibody (M00213-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of GFAP using anti-GFAP antibody (M00213-1).
GFAP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GFAP Antibody (M00213-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Cell specific location of IL-1β in the lumbar spinal cord. Representative images of double immunofluorescence staining for IL-1β with NeuN, GFAP, or Iba1 are shown. At 60 d, increased expression of IL-1β was observed in the neuronal cytoplasm of ventral horn spinal cord of ALS mice, and low expression was found in CON mice a . Besides NeuN + /IL-1β + double positive cells, dramatic co-localization of IL-1β with activated GFAP + astrocytes and Iba1 + microglia in transgenic ALS mice were observed from 95 to 122 d b , c , d . Arrows indicate double-labelled cells. Scale bar 50 μm
Index in PubMed under a CC BY license. PMID: 35945502

IHC analysis of GFAP using anti-GFAP antibody (M00213-1).
GFAP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GFAP Antibody (M00213-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Cell-specific location of caspase-1 in the lumbar spinal cord. Representative images of double immunofluorescence staining for caspase-1 with NeuN, GFAP, or Iba1 are shown. At 60 d, caspase-1 was mainly expressed in the neuronal cytoplasm of ventral horn spinal cord of ALS mice, and low expression was found in CON mice a . Increased co-localization of caspase-1 with activated GFAP + astrocytes and Iba1 + microglia in ALS mice were observed from 95 to 122 d b , c , d . Arrows indicate double-labelled cells. Scale bar 50 μm
Index in PubMed under a CC BY license. PMID: 35945502

Immunofluorescent analysis of SH-SY5Y cells, using GFAP Antibody .

Cell-specific location of NLRP3 in the lumbar spinal cord. Representative images of double immunofluorescence staining for NLRP3 with NeuN, GFAP, or Iba1 are shown. At 60 d, NLRP3 was mainly expressed in the neuronal cytoplasm of ventral horn spinal cord of ALS mice, and low expression was found in CON mice a . Dramatic co-localization with neurons and activated GFAP + astrocytes in ALS mice was observed at 95 d b . Increased NLRP3 expression was observed in GFAP + and Iba1 + cells in the ALS spinal cord c , d . Arrows indicate double-labelled cells. Scale bar 50 μm
Index in PubMed under a CC BY license. PMID: 35945502