Anti-GFAP Rabbit Monoclonal Antibody

Boster Bio Anti-GFAP Rabbit Monoclonal Antibody catalog # M00213-1. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 61 publication(s).

Product Info Summary

SKU: M00213-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-GFAP Rabbit Monoclonal Antibody

See all GFAP products

SKU/Catalog Number

M00213-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-GFAP Rabbit Monoclonal Antibody catalog # M00213-1. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GFAP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00213-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DBI-7

Immunogen

A synthesized peptide derived from human GFAP

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00213-1 is reactive to GFAP in Human, Mouse, Rat

Applications

M00213-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:10000-1:50000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30

Validation Images & Assay Conditions

Gene/Protein Information For GFAP (Source: Uniprot.Org, NCBI)

Uniprot ID

P14136

Gene ID

2670

Gene Name

GFAP

Full Name

Glial fibrillary acidic protein

Weight

49.88kDa

Superfamily

intermediate filament family

Alternative Names

FLJ45472; GFAP astrocytes; GFAP immunohistochemistry; GFAP mouse; GFAP rabbit; GFAP stain; GFAP; glial fibrillary acidic protein GFAP ALXDRD glial fibrillary acidic protein glial fibrillary acidic protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GFAP, check out the GFAP Infographic

GFAP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GFAP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00213-1 has been cited in 61 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Deng J,Xu T,Yang J,Zhang KM,Li Q,Yu XY,Li R,Fu J,Jiang Q,Ma JX,Chen YM.Sema7A, a brain immune regulator, regulates seizure activity in PTZ-kindled epileptic rats.CNS Neurosci Ther.2020 Jan;26(1):101-116.doi:10.1111/cns.13181.Epub 2019 Jun 9.PMID:31179640;
Species: Human,Rat
M00213-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100

Chen X,Zhang L,Hua F,Zhuang Y,Liu H,Wang S.EphA4 Obstructs Spinal Cord Neuron Regeneration by Promoting Excessive Activation of Astrocytes.Cell Mol Neurobiol.2021 Feb 17.doi:10.1007/s10571-021-01046-x.Epub ahead of print.PMID:33595805.
Species: Mouse
M00213-1 usage in article: APP:IF, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:500

Zhang LY,Jin QQ,Hölscher C,Li L.Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model. Neural Regen Res 2021;16:1660-70
Species: Human,Rat
M00213-1 usage in article: APP:IF, SAMPLE:SN TISSUE, DILUTION:1:100

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
M00213-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020, PREPRINT (Version 1) available at Research Square [https:/
Species: Mouse
M00213-1 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Ahmed S. Ahmed,JAK-1/STAT-3 pathway mediated role in aging cerebellar cortex degenerative changes of albino wistar rats, Translational Research in Anatomy, 2020,100089,ISSN 2214-854X, https://doi.org/10. 1016/j.tria.2020.100089.
Species: Rat
M00213-1 usage in article: APP:IHC, SAMPLE:CEREBELLAR CORTEX, DILUTION:1:20

Zhou Feng,Shengyan Liu,Qianwei Chen,Qiang Tan,Jishu Xian,Hua Feng,Zhi Chen,Gang Li,uPA alleviates kaolin-induced hydrocephalus by promoting the release and activation of hepatocyte growth factor in rats, Neuroscience Letters,Volume 731,2020,135011,ISSN 03
Species: Rat

Jian Wang,Xiaolin Li,Yu Song,Qiangfei Su,Xiakeerzhati Xiaohalati,Wen Yang,Luming Xu,Bo Cai,Guobin Wang,Zheng Wang,Lin Wang,Injectable silk sericin scaffolds with programmable shape-memory property and neuro-differentiation-promoting activity for individua
Species: Silkworm,Mouse
M00213-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100

Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020,PREPRINT (Version 1)available at Research Square [+https://
Species: Mouse
M00213-1 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Luo Z,Wang J,Tang S,Zheng Y,Zhou X,Tian F,Xu Z.Dynamic-related protein 1 inhibitor eases epileptic seizures and can regulate equilibrative nucleoside transporter 1 expression.BMC Neurol.2020 Sep 22;20(1):353.doi:10.1186/s12883-020-01921-y.PMID:32962663;PM
Species: Rat
M00213-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Wang X,Tong Y,Zhang J,Khan N,Zhang K,Bai H,Zhang Q,Chen Y.Neuroinflammation changes with periodontal inflammation status during periodontitis in wild-type mice.Oral Dis.2020 Aug 20.doi:10.1111/odi.13618.Epub ahead of print.PMID:32815656.
Species: Mouse
M00213-1 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:50

Wang X,Zhou W,Li X,Ren J,Ji G,Du J,Tian W,Liu Q,Hao A. Graphene oxide suppresses the growth and malignancy of glioblastoma stem cell-like spheroids via epigenetic mechanisms. J Transl Med.2020 May 14;18(1):200. doi:10.1186/s12967-020-02359-z.PMID:32410622
Species: Human
M00213-1 usage in article: APP:WB, SAMPLE:GSCS, DILUTION:1:1000

Li R,Wen Y,Wu B,He M,Zhang P,Zhang Q,Chen Y.MicroRNA-25-3p suppresses epileptiform discharges through inhibiting oxidative stress and apoptosis via targeting OXSR1 in neurons.Biochem Biophys Res Commun. 2020 Mar 19;523(4):859-866.doi:10.1016/j.bbrc.2020.
Species: Rat
M00213-1 usage in article: APP:IHC, SAMPLE:HIPPOCAMPUL NEURONS, DILUTION:NA

Deng Z,Wei Y,Yao Y,Gao S,Wang X.Let-7f promotes the differentiation of neural stem cells in rats.Am J Transl Res.2020 Sep 15;12(9):5752-5761.PMID:33042454;PMCID:PMC7540113.
Species: Rat
M00213-1 usage in article: APP:IF, SAMPLE:NSCS, DILUTION:1:100

Li X,Shi MQ,Chen C,Du JR.Phthalide derivative CD21 ameliorates ischemic brain injury in a mouse model of global cerebral ischemia: involvement of inhibition of NLRP3.Int Immunopharmacol.2020 Sep;86:106714.doi: 10.1016/j.intimp.2020.106714.Epub 2020 Jun 24
Species: Mouse
M00213-1 usage in article: APP:IF, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:100

Yang Z,Bao Y,Chen W,He Y.Melatonin exerts neuroprotective effects by attenuating astro- and microgliosis and suppressing inflammatory response following spinal cord injury.Neuropeptides.2020 Feb;79:102002.doi: 10.1016/j.npep.2019.102002.Epub 2019 Dec 25.P
Species: Mouse
M00213-1 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:500

Xue NY,Ge DY,Dong RJ,Kim HH,Ren XJ,Tu Y.Effect of electroacupuncture on glial fibrillary acidic protein and nerve growth factor in the hippocampus of rats with hyperlipidemia and middle cerebral artery thrombus. Neural Regen Res.2021 Jan;16(1):137-142.doi
Species: Rat
M00213-1 usage in article: APP:IHC, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:100

Zhu X,Yao Y,Yang J,Zhengxie J,Li X,Hu S,Zhang A,Dong J,Zhang C,Gan G.COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice. Int Immunopharmacol. 2020 Oct;87:106801.doi: 10.1016/j.inti
Species: Mouse
M00213-1 usage in article: APP:ICC, SAMPLE:BRAIN TISSUE, DILUTION:1:100

Zhou Z,Hou J,Mo Y,Ren M,Yang G,Qu Z,Hu Y.Geniposidic acid ameliorates spatial learning and memory deficits and alleviates neuroinflammation via inhibiting HMGB-1 and downregulating TLR4/2 signaling pathway in APP/PS1 mice.Eur J Pharmacol.2020 Feb 15;869:1
Species: Mouse
M00213-1 usage in article: APP:IHC, SAMPLE:BRAIN SECTION, DILUTION:1:200

Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Zhang C,Yang H. Increased Expression of Fibroblast Growth Factor 13 in Cortical Lesions of the Focal Cortical Dysplasia. Brain Res Bull. 2020 Dec 4:S0361-9230(20)30710-3.doi: 10.1016/j.brainresbull.2020.11.023.Epub ahead
Species: Human
M00213-1 usage in article: APP:IHC, SAMPLE:BRAIN TISSU E, DILUTION:1:50

The Novel Dual GLP-1/GIP Receptor Agonist DA-CH5 Is Superior to Single GLP-1 Receptor Agonists in the MPTP Model of Parkinson's Disease Lingyu Zhang J Parkinson's Dis. 2020;10(2):523-542. DOI: 10.3233/JPD-191768.

Metalloprotease Adam10 suppresses epilepsy through repression of hippocampal neuroinflammation

Activation of LILRB2 signal pathway in temporal lobe epilepsy patients and in a pilocarpine induced epilepsy model

Neuroprotective effects of a GIP analogue in the MPTP Parkinson's disease mouse model

Neuroprotective Effects of Pycnogenol Against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in Primary Rat Astrocytes via NF-%u03BAB and ERK1/2 MAPK %u2026

Tumor-associated microglia/macrophages enhance the invasion of glioma stem-like cells via TGF-%u03B21 signaling pathway

Local injection of bone morphogenetic protein 7 promotes neuronal regeneration and motor function recovery after acute spinal cord injury

Inhibition of P2X7 receptors improves outcomes after traumatic brain injury in rats

Water-soluble ginseng oligosaccharides protect against scopolamine-induced cognitive impairment by functioning as an antineuroinflammatory agent

Reversion of malignant phenotypes of human glioblastoma cells by ?-elemene through ?-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules

The Neuropilin-1 Inhibitor, ATWLPPR Peptide, Prevents Experimental Diabetes-Induced Retinal Injury by Preserving Vascular Integrity and Decreasing Oxidative Stress

Brain Localization and Neurotoxicity Evaluation of Polysorbate 80-Modified Chitosan Nanoparticles in Rats

Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats

Loss of the golgin GM130 causes Golgi disruption, Purkinje neuron loss, and ataxia in mice

In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats

The association and significance of H3K27me3 and a folate metabolic gene ACat2 in neural tube defects

Cyclin-dependent kinase inhibitor flavopiridol promotes remyelination in a cuprizone induced demyelination model

Evaluation of the Combination of Methylprednisolone and Tranilast after Spinal Cord Injury in Rat Models

Effect of Corilagin on the Proliferation and NF-?B in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells

GDNF Enhances Therapeutic Efficiency of Neural Stem Cells-Based Therapy in Chronic Experimental Allergic Encephalomyelitis in Rat

The hGFAP-driven conditional TSPO knockout is protective in a mouse model of multiple sclerosis

Transplantation of Neural Stem Cells Cotreated with Thyroid Hormone and GDNF Gene Induces Neuroprotection in Rats of Chronic Experimental Allergic Encephalomyelitis

Re-infection of the prion from the scrapie?infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c

Effect of siRNA-Livin on drug resistance to chemotherapy in glioma U251 cells and CD133+ stem cells

Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro

Histidine provides long-term neuroprotection after cerebral ischemia through promoting astrocyte migration

Different methods for inducing adipose-derived stem cells to differentiate into Schwann-like cells

Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer?s Disease-Like Amyloid-? Pathology and Neuroinflammation in the Rat Hippocampus

Acrylamide neurotoxicity on the cerebrum of weaning rats

Optimal electroacupuncture frequency for maintaining astrocyte structural integrity in cerebral ischemia

Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke

Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia

Wang Y, Huang N, Li H, Liu S, Chen X, Yu S, Wu N, Bian XW, Shen HY, Li C, Xiao L. Oncotarget. 2017 Jun 6;8(23):37511-37524. doi: 10.18632/oncotarget.16400. Promoting oligodendroglial-oriented differentiation of glioma stem cell: a repurposing of q...

Zhang M, Chai Y, Liu T, Xu N, Yang C. Exp Ther Med. 2015 Apr;9(4):1141-1148. Epub 2015 Feb 2. Synergistic Effects Of Buyang Huanwu Decoction And Embryonic Neural Stem Cell Transplantation On The Recovery Of Neurological Function In A Rat Model Of ...

Li T, Mo X, Jiang Z, He W, Lu W, Zhang H, Zhang J, Zeng L, Yang B, Xiao H, Hu Z. Oxid Med Cell Longev. 2012;2012:945071. Doi: 10.1155/2012/945071. Epub 2012 Oct 12. Study Of ??b-Crystallin Expression In Gerbil Bcao Model Of Transient Global Cerebr...

Bai Y, Xu G, Xu M, Li Q, Qin X. Int J Mol Med. 2014 Dec;34(6):1473-82. Doi: 10.3892/Ijmm.2014.1946. Epub 2014 Sep 24. Inhibition Of Src Phosphorylation Reduces Damage To The Blood-Brain Barrier Following Transient Focal Cerebral Ischemia In Rats.

Zhang X, Zheng Z, Shu B, Liu X, Zhang Z, Liu Y, Bai B, Hu Q, Mao P, Wang H. J Virol. 2013 Nov;87(22):12407-21. Doi: 10.1128/Jvi.02090-13. Epub 2013 Sep 11. Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection.

Wang R, Zhang X, Zhang J, Fan Y, Shen Y, Hu W, Chen Z. Plos One. 2012;7(5):E37574. Doi: 10.1371/Journal.Pone.0037574. Epub 2012 May 22. Oxygen-Glucose Deprivation Induced Glial Scar-Like Change In Astrocytes.

Ma H, Yu B, Kong L, Zhang Y, Shi Y. Mol Med Rep. 2011 Sep-Oct;4(5):849-56. Doi: 10.3892/Mmr.2011.510. Epub 2011 Jun 16. Transplantation Of Neural Stem Cells Enhances Expression Of Synaptic Protein And Promotes Functional Recovery In A Rat Model Of...

Chen Y, Wu X, Yu S, Lin X, Wu J, Li L, Zhao J, Zhao Y. Plos One. 2012;7(6):E40165. Doi: 10.1371/Journal.Pone.0040165. Epub 2012 Jun 29. Neuroprotection Of Tanshinone Iia Against Cerebral Ischemia/Reperfusion Injury Through Inhibition Of Macrophage...

Yu Q, Guo Z, Liu X, Ouyang Q, He C, Burnstock G, Yuan H, Xiang Z. Purinergic Signal. 2013 Dec;9(4):663-75. Doi: 10.1007/S11302-013-9379-Y. Epub 2013 Jul 23. Block Of P2X7 Receptors Could Partly Reverse The Delayed Neuronal Death In Area Ca1 Of The...

Have you used Anti-GFAP Rabbit Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-GFAP Rabbit Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

18 Customer Q&As for Anti-GFAP Rabbit Monoclonal Antibody

Question

Is there a BSA free version of anti-GFAP Rabbit Monoclonal antibody M00213-1 available?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GFAP Rabbit Monoclonal antibody M00213-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-09

Question

We have seen staining in rat brain thalamus. Are there any suggestions? Is anti-GFAP Rabbit Monoclonal antibody supposed to stain brain thalamus positively?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

From literature brain thalamus does express GFAP. From Uniprot.org, GFAP is expressed in dorsal motor nucleus of vagus nerve, brain thalamus, brain, kidney, fetal brain cortex, fetal brain, blood, among other tissues. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2020-01-09

Question

I see that the anti-GFAP Rabbit Monoclonal antibody M00213-1 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-23

Question

I am interested in using your anti-GFAP Rabbit Monoclonal antibody for nuclear signaling by erbb4 studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

Thanks for your inquiry. This M00213-1 anti-GFAP Rabbit Monoclonal antibody is validated on rat brain tissue, mouse brain. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-29

Question

We ordered your anti-GFAP Rabbit Monoclonal antibody for IF on blood in the past. I am using human, and We want to use the antibody for WB next. My lab would like examining blood as well as kidney in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

I took a look at the website and datasheets of our anti-GFAP Rabbit Monoclonal antibody and I see that M00213-1 has been validated on human in both IF and WB. Thus M00213-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-21

Question

We are currently using anti-GFAP Rabbit Monoclonal antibody M00213-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

The anti-GFAP Rabbit Monoclonal antibody (M00213-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-15

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-GFAP Rabbit Monoclonal antibody M00213-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-15

Question

Has M00213-1 been tested for Flow Cytometry application?

Verified customer

Asked: 2019-07-04

Answer

The Anti-GFAP Rabbit Monoclonal Antibody (M00213-1) can't work for Flow Cytometry application.

Boster Scientific Support

Answered: 2019-07-05

Question

Can FITC or Alexfluor 488 secondary antibodies work for M00213-1?

Verified customer

Asked: 2019-07-04

Answer

FITC or Alexfluor 488 secondary antibodies could work for the Anti-GFAP Rabbit Monoclonal Antibody (M00213-1).

Boster Scientific Support

Answered: 2019-07-04

Question

Does anti-GFAP Rabbit Monoclonal antibody M00213-1 work for IF with kidney?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

According to the expression profile of kidney, GFAP is highly expressed in kidney. So, it is likely that anti-GFAP Rabbit Monoclonal antibody M00213-1 will work for IF with kidney.

Boster Scientific Support

Answered: 2019-06-21

Question

Will M00213-1 anti-GFAP Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

As indicated on the product datasheet, M00213-1 anti-GFAP Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-05

Question

I have a question about product M00213-1, anti-GFAP Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00213-1 anti-GFAP Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-28

Question

Is a blocking peptide available for product anti-GFAP Rabbit Monoclonal antibody (M00213-1)?

Verified Customer

Verified customer

Asked: 2018-12-14

Answer

We do provide the blocking peptide for product anti-GFAP Rabbit Monoclonal antibody (M00213-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-12-14

Question

I was wanting to use your anti-GFAP Rabbit Monoclonal antibody for IF for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?

Verified Customer

Verified customer

Asked: 2017-07-25

Answer

You can see on the product datasheet, M00213-1 anti-GFAP Rabbit Monoclonal antibody has been validated for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-07-25

Question

Here is the WB image, lot number and protocol we used for kidney using anti-GFAP Rabbit Monoclonal antibody M00213-1. Please let me know if you require anything else.

D. Carter

Verified customer

Asked: 2014-10-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-10-28

Question

Is this M00213-1 anti-GFAP Rabbit Monoclonal antibody reactive to the isotypes of GFAP?

D. Rodriguez

Verified customer

Asked: 2014-08-26

Answer

The immunogen of M00213-1 anti-GFAP Rabbit Monoclonal antibody is A synthesized peptide derived from human GFAP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-08-26

Question

My team were satisfied with the WB result of your anti-GFAP Rabbit Monoclonal antibody. However we have observed positive staining in brain cytoplasm. using this antibody. Is that expected? Could you tell me where is GFAP supposed to be expressed?

Z. Roberts

Verified customer

Asked: 2013-03-27

Answer

Based on literature, brain does express GFAP. Generally GFAP expresses in cytoplasm. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2013-03-27

Question

My lab would like to test anti-GFAP Rabbit Monoclonal antibody M00213-1 on human kidney for research purposes, then I may be interested in using anti-GFAP Rabbit Monoclonal antibody M00213-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

A. Parker

Verified customer

Asked: 2013-02-25

Answer

The products we sell, including anti-GFAP Rabbit Monoclonal antibody M00213-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-02-25

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M00213-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.