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Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Application:ELISA, IHC, WB
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Product Name Anti-CNTF Picoband™ Antibody
SKU/Catalog Number PB9067
Description Rabbit IgG polyclonal antibody for Ciliary neurotrophic factor(CNTF) detection. Tested with WB, IHC-P, ELISA in Mouse;Rat.
Cite This Product Anti-CNTF Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9067)
Replacement Item This antibody may replace the following items: sc-13996|sc-1912|sc-365211|sc-166272|sc-365210|sc-1911|sc-166273|sc-25286|sc-1912-R from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Mouse, Rat
Application ELISA, IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E. coli-derived rat CNTF recombinant protein (Position: A2-M200). Rat CNTF shares 84% and 95% amino acid (aa) sequences identity with human and mouse CNTF, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cntf
Protein Name Ciliary neurotrophic factor
Molecular Weight 22854 MW
Protein Function CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Tissue Specificity Nervous system.
Subcellular Localization Cytoplasm.
Uniprot ID P20294
Alternative Names Ciliary neurotrophic factor;CNTF;Cntf;
Research Areas |neuroscience|neurology process|growth and development|neurotrophins| neuroscience|cell type marker|neural stem cell marker| stem cells|neural stem cells|glial restricted lineage|glial progenitors|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Ciliary neurotrophic factor

Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene. The protein encoded by this gene is a polypeptide hormone andnerve growth factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neural populations includingastrocytes. It is mapped to 11q12.1. In addition to neurotrophic effects on parasympathetic neurons, CNTF was shown to have activities on sympathetic and sensory neurons. CNTF appears not to be involved in motor neuron survival during development. human CNTF has a trophic influence on degenerating striatal neurons as well as on critical nonstriatal regions. CNTF plays a role in a cardiac signal transduction pathway that regulates obesity-related left ventricular hypertrophy.

Anti-CNTF Picoband™ Antibody Images

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Anti-CNTF Picoband™ Antibody
Anti-CNTF Picoband antibody, PB9067-1.JPG
IHC(P): Mouse Brain Tissue
Anti-CNTF Picoband™ Antibody
Anti-CNTF Picoband antibody, PB9067-2.JPG
IHC(P): Rat Brain Tissue
Anti-CNTF Picoband™ Antibody
Anti-CNTF Picoband antibody, PB9067-3.jpg
All lanes: Anti-CNTF(PB9067) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: NRK Whole Cell Lysate at 40ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Lane 4: Rat Brain Tissue Lysate at 40ug
Lane 5: Rat Liver Tissue Lysate at 40ug
Lane 6: Rat Spleen Tissue Lysate at 40ug
Lane 7: Rat Testis Tissue Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.