Click image to see more details
Product Info Summary
| SKU: | M30939 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GFP Rabbit Monoclonal Antibody
SKU/Catalog Number
M30939
BM3883 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GFP Rabbit Monoclonal Antibody catalog # M30939. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GFP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30939)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CE-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from green fluorescent protein
Reactive Species
M30939 is reactive to GFP in Human, Mouse, Rat
Observed Molecular Weight
27 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30939 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: 293 cell
IHC: GFP transgenic human colon tissue
ICC/IF: 293 cell
Validation Images & Assay Conditions
Click image to see more details
MCMV infection influence the lipid metabolism pathway in infant mice hepatocytes. (A) The hepatocytes cell cluster in Figure 1A were selected. Enriched GO terms for significantly down-regulated genes in hepatocytes cluster, compared with other clusters, were shown as bubble diagram. (B) Hepatocytes from three uninfected and three MCMV infected 2 weeks old WT mice were isolated and proteomic mass spectrometry analysis were performed individually. PCA analysis showed that the difference between infection group and control group. Each point represents an individual mice. (C) Enriched KEGG terms for significantly down-regulated genes of hepatocytes between uninfected and infected group in proteomic mass spectrometry analysis. (D) Proteomic gene regulation network of down-regulated PPAR-γ pathway analyzed by STRING. (E) WT mice were infected by MCMV at 14 days post birth. Immunoblotting with antibodies target ACOX1, ACSL1, FABP1, SLC27A2 and GFP in hepatocytes from mice infected with MCMV at three dpi or treated with PBS. Experiments were repeated three times. Each lane represents an individual mice. (F) Quantitative analysis of (E) (mean ± SD). Statistical significance was determined by non-parametric Mann Whitney test between groups (*P < 0.05, ***P < 0.001). (G) AML12 mice hepatocyte cell were transfected with siRNA that does not match any known genes or regulatory regions(siControl) or target Pparg mRNA(si Pparg ). qPCR analysis of indicated genes in different cells at 24 hours post MCMV infection (n=4, mean ± SD). Statistical significance was determined by non-parametric Mann Whitney test between groups (*P < 0.05, ***P < 0.001). Experiments were repeated three times. Each point represents an individual cell sample.
Index in PubMed under a CC BY license. PMID: 37638012
Click image to see more details
Immunohistochemical analysis of paraffin-embedded GFP transgenic human colon, using GFP Antibody.
Click image to see more details
Immunofluorescent analysis of 293 cells, using GFP Antibody.
Click image to see more details
Western blot analysis of GFP expression in 293 cell lysate transfected with GFP.
Specific Publications For Anti-GFP Rabbit Monoclonal Antibody (M30939)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GFP Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-GFP Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
