Anti-Myelin Basic Protein/MBP Antibody

Boster Bio Anti-Myelin Basic Protein/MBP Antibody catalog # PA1050. Tested in IF, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 15 publication(s).

Product Info Summary

SKU: PA1050
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC-P, WB

Product Name

Anti-Myelin Basic Protein/MBP Antibody

See all MBP products

SKU/Catalog Number

PA1050

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Myelin Basic Protein/MBP Antibody catalog # PA1050. Tested in IF, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Myelin Basic Protein/MBP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1050)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein (182-197aa KLGGRDSRSGSPMARR), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1050 is reactive to MBP in Human, Mouse, Rat

Applications

PA1050 is guaranteed for IF, IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Immunofluorescence, 2μg/ml, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For MBP (Source: Uniprot.Org, NCBI)

Uniprot ID

P02686

Gene ID

4155

Gene Name

MBP

Full Name

Myelin basic protein

Weight

33.117kDa

Superfamily

myelin basic protein family

Alternative Names

Hmbpr; MBP; MGC99675; mld; Myelin A1 protein; myelin basic protein; Myelin membrane encephalitogenic protein; shi MBP myelin basic protein myelin basic protein|Golli-MBP|proline-rich proteoglycan 2|myelin A1 protein|myelin membrane encephalitogenic protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MBP, check out the MBP Infographic

MBP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MBP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1050 has been cited in 15 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Meng FW,Jing XN,Song GH,Jie LL,Shen FF.Prox1 induces new lymphatic vessel formation and promotes nerve reconstruction in a mouse model of sciatic nerve crush injury.J Anat.2020 Nov;237(5):933-940.doi: 10.1111/joa.13247.Epub 2020 Jun 9.PMID:32515838;PMCID:
Species: Mouse
PA1050 usage in article: APP:IF, SAMPLE:SCIATIC NERVE TISSUE, DILUTION:1:100

Nexilin Regulates Oligodendrocyte Progenitor Cell Migration and Remyelination and is Negatively Regulated by PAR1/RAP1 Signaling Following Subarachnoid %u2026

Cyclin-dependent kinase inhibitor flavopiridol promotes remyelination in a cuprizone induced demyelination model

Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro

Zhou XB, Liu N, Wang D, Zou DX, Wei CW, Zhou JL. Neural Regen Res. 2018 Mar;13(3):492-496. doi: 10.4103/1673-5374.228733. Neuroprotective effect of ischemic postconditioning on sciatic nerve transection

Zhang M, Chai Y, Liu T, Xu N, Yang C. Exp Ther Med. 2015 Apr;9(4):1141-1148. Epub 2015 Feb 2. Synergistic Effects Of Buyang Huanwu Decoction And Embryonic Neural Stem Cell Transplantation On The Recovery Of Neurological Function In A Rat Model Of ...

Gao S, Zheng Y, Cai Q, Yao W, Wang J, Zhang P, Wang X. J Mater Sci Mater Med. 2014 May;25(5):1283-91. Doi: 10.1007/S10856-014-5150-3. Epub 2014 Jan 23. Comparison Of Morphology And Biocompatibility Of Acellular Nerve Scaffolds Processed By Differe...

Wang B, Xiao Z, Chen B, Han J, Gao Y, Zhang J, Zhao W, Wang X, Dai J. Plos One. 2008 Mar 26;3(3):E1856. Doi: 10.1371/Journal.Pone.0001856. Nogo-66 Promotes The Differentiation Of Neural Progenitors Into Astroglial Lineage Cells Through Mtor-Stat3 ...

Jin Sj, Liu Y, Deng Sh, Liao Lh, Lin Tl, Ning Q, Luo Xp. Int J Mol Med. 2015 May;35(5):1199-212. Doi: 10.3892/Ijmm.2015.2136. Epub 2015 Mar 13. Neuroprotective Effects Of Activated Protein C On Intrauterine Inflammation-Induced Neonatal White Matt...

Wei J, Zheng M, Liang P, Wei Y, Yin X, Tang Y, Xue Y. Neurobiol Dis. 2013 Aug;56:59-65. Doi: 10.1016/J.Nbd.2013.04.009. Epub 2013 Apr 22. Apolipoprotein E And Its Mimetic Peptide Suppress Th1 And Th17 Responses In Experimental Autoimmune Encephalo...

Chen X,, Chen X, Huang X, Qin C, Fang Y, Liu Y, Zhang G, Pan D, Wang W, Xie M. Mol Neurobiol. 2015 Feb 10. [Epub Ahead Of Print] Soluble Epoxide Hydrolase Inhibition Provides Multi-Target Therapeutic Effects In Rats After Spinal Cord Injury.

Han Sm, Wan H, Kudo G, Foltz Wd, Vines Dc, Green De, Zoerle T, Tariq A, Brathwaite S, D'Abbondanza J, Ai J, Macdonald Rl. J Cereb Blood Flow Metab. 2014 Jan;34(1):108-17. Doi: 10.1038/Jcbfm.2013.170. Epub 2013 Sep 25. Molecular Alterations In The ...

Yang P, Gao Z, Zhang H, Fang Z, Wu C, Xu H, Huang Qj. Neuropsychiatr Dis Treat. 2015 Mar 6;11:597-607. Doi: 10.2147/Ndt.S78131. Ecollection 2015. Changes In Proinflammatory Cytokines And White Matter In Chronically Stressed Rats.

Wang X, Tu Q, Zhao B, An Y, Wang Jc, Liu W, Yuan Ms, Ahmed Sm, Xu J, Liu R, Zhang Y, Wang J. Biomaterials. 2013 Jan;34(4):1155-69. Doi: 10.1016/J.Biomaterials.2012.10.063. Epub 2012 Nov 17. Effects Of Poly(L-Lysine)-Modified Fe3O4 Nanoparticles On...

Ping Yf, Yao Xh, Jiang Jy, Zhao Lt, Yu Sc, Jiang T, Lin Mc, Chen Jh, Wang B, Zhang R, Cui Yh, Qian C, Wang Jm, Bian Xw. J Pathol. 2011 Jul;224(3):344-54. Doi: 10.1002/Path.2908. Epub 2011 May 27. The Chemokine Cxcl12 And Its Receptor Cxcr4 Promote...

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17 Customer Q&As for Anti-Myelin Basic Protein/MBP Antibody

Question

We have observed staining in mouse amygdala. Any tips? Is anti-Myelin Basic Protein/MBP antibody supposed to stain amygdala positively?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

From what I have seen in literature amygdala does express MBP. From what I have seen in Uniprot.org, MBP is expressed in c1 segment of cervical spinal cord, embryonic spinal cord, brain, amygdala, hippocampus subthalamic nucleus, brain, lung skin, leukemic t-cell, cervix carcinoma, among other tissues. Regarding which tissues have MBP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 7504278, 7574672, 7685161
Cervix carcinoma, Pubmed ID: 23186163
Embryonic spinal cord, Pubmed ID: 2427738, 2442403
Hippocampus, and Subthalamic nucleus, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2020-04-16

Question

Will anti-Myelin Basic Protein/MBP antibody PA1050 work on goat IF with amygdala?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

Our lab technicians have not tested anti-Myelin Basic Protein/MBP antibody PA1050 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Myelin Basic Protein/MBP antibody PA1050 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat amygdala in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-06

Question

Our lab want to know about using your anti-Myelin Basic Protein/MBP antibody for myelination studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

R. Yang

Verified customer

Asked: 2020-03-16

Answer

Thanks for your inquiry. This PA1050 anti-Myelin Basic Protein/MBP antibody is tested on mouse brain, brain tissue, rat brain tissue. It is guaranteed to work for IF, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-16

Question

I was wanting to use your anti-Myelin Basic Protein/MBP antibody for IHC-P for rat lung skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat lung skin identification?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

You can see on the product datasheet, PA1050 anti-Myelin Basic Protein/MBP antibody has been tested for IF, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung skin in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-27

Question

We ordered your anti-Myelin Basic Protein/MBP antibody for WB on hippocampus subthalamic nucleus in the past. I am using human, and We want to use the antibody for IHC-P next. I would like examining hippocampus subthalamic nucleus as well as amygdala in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

I looked at the website and datasheets of our anti-Myelin Basic Protein/MBP antibody and I see that PA1050 has been validated on human in both WB and IHC-P. Thus PA1050 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-26

Question

I am interested in to test anti-Myelin Basic Protein/MBP antibody PA1050 on rat lung skin for research purposes, then I may be interested in using anti-Myelin Basic Protein/MBP antibody PA1050 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

The products we sell, including anti-Myelin Basic Protein/MBP antibody PA1050, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-18

Question

Will anti-Myelin Basic Protein/MBP antibody PA1050 work for IHC-P with lung skin?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

According to the expression profile of lung skin, MBP is highly expressed in lung skin. So, it is likely that anti-Myelin Basic Protein/MBP antibody PA1050 will work for IHC-P with lung skin.

Boster Scientific Support

Answered: 2019-06-26

Question

Our team were happy with the WB result of your anti-Myelin Basic Protein/MBP antibody. However we have been able to see positive staining in hippocampus subthalamic nucleus myelin membrane using this antibody. Is that expected? Could you tell me where is MBP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

Based on literature, hippocampus subthalamic nucleus does express MBP. Generally MBP expresses in myelin membrane. Regarding which tissues have MBP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 7504278, 7574672, 7685161
Cervix carcinoma, Pubmed ID: 23186163
Embryonic spinal cord, Pubmed ID: 2427738, 2442403
Hippocampus, and Subthalamic nucleus, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2019-06-17

Question

Is a blocking peptide available for product anti-Myelin Basic Protein/MBP antibody (PA1050)?

Verified Customer

Verified customer

Asked: 2019-05-09

Answer

We do provide the blocking peptide for product anti-Myelin Basic Protein/MBP antibody (PA1050). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-09

Question

Is this PA1050 anti-Myelin Basic Protein/MBP antibody reactive to the isotypes of MBP?

C. Walker

Verified customer

Asked: 2018-05-11

Answer

The immunogen of PA1050 anti-Myelin Basic Protein/MBP antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein(182-197aa KLGGRDSRSGSPMARR), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-05-11

Question

Do you have a BSA free version of anti-Myelin Basic Protein/MBP antibody PA1050 available?

Verified Customer

Verified customer

Asked: 2017-12-04

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Myelin Basic Protein/MBP antibody PA1050 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-12-04

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung skin using anti-Myelin Basic Protein/MBP antibody PA1050. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-11-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-15

Question

We are currently using anti-Myelin Basic Protein/MBP antibody PA1050 for rat tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

D. Yang

Verified customer

Asked: 2017-10-12

Answer

The anti-Myelin Basic Protein/MBP antibody (PA1050) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-12

Question

My question regarding product PA1050, anti-Myelin Basic Protein/MBP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

D. Moore

Verified customer

Asked: 2017-06-09

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1050 anti-Myelin Basic Protein/MBP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-06-09

Question

I see that the anti-Myelin Basic Protein/MBP antibody PA1050 works with IHC-P, what is the protocol used to produce the result images on the product page?

P. Wu

Verified customer

Asked: 2016-09-14

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-09-14

Question

See attached the WB image, lot number and protocol we used for lung skin using anti-Myelin Basic Protein/MBP antibody PA1050. Please let me know if you require anything else.

E. Li

Verified customer

Asked: 2014-09-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-09-25

Question

Would PA1050 anti-Myelin Basic Protein/MBP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

K. Taylor

Verified customer

Asked: 2014-02-04

Answer

As indicated on the product datasheet, PA1050 anti-Myelin Basic Protein/MBP antibody as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-02-04

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PA1050
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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