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MBP antibody (PA1050) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Pig, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 66 Publications

pa1050 mbp primary antibodies if testing 1

IF analysis of MBP using anti-MBP antibody (PA1050).
MBP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MBP Antibody (PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-Myelin Basic Protein/MBP Antibody Picoband®

Name: Anti-Myelin Basic Protein/MBP Antibody Picoband®
Brand: Boster Bio
SKU: PA1050
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

MBP antibody

66 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Pig, Rat	Rabbit	IF, IHC, WB

Tested Applications

MBP antibody was positively detected by WB in:

rat brain tissue, mouse brain tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MBP antibody was positively detected by IHC in:

human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

MBP antibody was positively detected by IF in:

human brain tissue, mouse brain tissue, rat brain tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Rat

Tested

Mouse

Tested

Human

Tested

Pig

Tested

Recommend

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-Myelin Basic Protein/MBP Antibody Picoband®
View all MBP Antibodies

Form

Lyophilized

Uniprot

P02686

Gene ID

4155

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein, identical to the related rat and mouse sequences.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

15-22 kDa

Calculated Molecular Weight

33.1 kDa

Background of MBP

Myelin basic protein (MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.

Cite This Product

Anti-Myelin Basic Protein/MBP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1050)

Protocols

Western Blot protocol for MBP antibody

Review Protocol

Immunohistochemistry (IHC) protocol for MBP antibody

Review Protocol

Immunofluorescence (IF) protocol for MBP antibody

Review Protocol

Publications

Deletion of wfs1 Impairs Oligodendrocyte Precursor Cells Dorsal Distribution and Myelination Through the wfs1-hmgcs1 Axis in Zebrafish

Published: 2026-08-16

Journal: Biology-Basel

Application: WB

Impact Factor: 4.30

Species: Zebrafish

PMID: 42651706

SKU	PA1050
Application	Immunofluorenscence
Sample	Mouse spinal cord
Sample Processing Description	Mouse spinal cord fixed with 2% paraformaldehyde for 6–8 hours, dehydrated in 30% sucrose, embedded in OCT, and sectioned using a cryostat.
Primary Antibody	Anti-MBP9 antibody
Primary Incubation	1:100, overnight at 4 ℃
Blocking Agent	3% BSA
Secondary Antibody	Anti-rabbit IgG-CY3 conjugated antibody.
Secondary Incubation	Incubate at room temperature for 1 hour
Detection	Immunofluorescence images were acquired using a confocal laser microscope (Leica SP8, Zeiss LSM 880 Airyscan).
Results Summary	BOSTER’s rabbit anti-MBP antibody (catalog BA0094) has high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study.

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Anti-Myelin Basic Protein/MBP Antibody Picoband®

MBP antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Deletion of wfs1 Impairs Oligodendrocyte Precursor Cells Dorsal Distribution and Myelination Through the wfs1-hmgcs1 Axis in Zebrafish

Forsythoside A alleviates experimental autoimmune encephalomyelitis by targeting Tnfaip2

Vitamin B6 modulates microglial phagocytosis after hemorrhagic stroke

Unveiling Remyelinating Properties of Roflumilast in CPZ-Induced Neuronal Demyelination in Mice

Meningeal blood vessel blockage enhances anti-glioblastoma immunity

NPD1/GPR37 signaling protects against painful traumatic brain injury and comorbidities by regulating demyelination, glial responses, and neuroinflammation in the mouse brain

COPII component Sec13 is required for peripheral myelination and Schwann cell maintenance

Berberine Ameliorates Postoperative Cognitive Dysfunction in Aged Mice and Regulates the PI3K‐AKT Pathway: A Network Pharmacology Study

Preventive and therapeutic effects of Tanshinone IIA on spinal cord injury without radiographic abnormality by regulating microglial phenotype polarization

Combined enriched environment and fluoxetine enhance myelin protein expression in the prefrontal cortex of a chronic unpredictable stress depression model

Genipin stabilized fibrin microbeads: Carrying cytokines to form niches for stem cell differentiation

Tanshinone IIA Promotes Functional Recovery After Spinal Cord Injury by Inhibiting Neuron and Oligodendrocyte Ferroptosis Through the GPX4/ACSL4 Axis

OCA-B promotes pathogenic maturation of stem-like CD4+ T cells and autoimmune demyelination

Diquat exposure causes brainstem demyelination by upregulating the mitochondrial calcium uniporter

Electroacupuncture promotes oligodendrocyte differentiation and myelin repair in a rat model of vascular dementia: Investigation of the mechanism from NF-κB-mediated inflammation

Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model

Transcranial near-infrared light promotes remyelination through AKT1/mTOR pathway to ameliorate postoperative neurocognitive disorder in aged mice

Can minor trauma cause asymptomatic cervical spinal cord compression leading to severe cervical spinal cord injury in rats?

Increased Thyroid Hormone Action Alleviates Hippocampal Damage by Downregulating Neuronal Type I Interferon Signaling/Necroptosis in Diabetes-Associated Cognitive Dysfunction

Selective bioaccumulation of polystyrene nanoplastics in fetal rat brain and damage to myelin development

Kv1.3 Blockade Alleviates White Matter Injury through Reshaping M1/M2 Phenotypes via the NF-κB Signaling Pathway after Intracerebral Hemorrhage

Tauroursodeoxycholic Acid Inhibited Apoptosis and Oxidative Stress in H2O2-Induced BMSC Death via Modulating the Nrf-2 Signaling Pathway: the Therapeutic Implications in a Rat Model of Spinal Cord Injury

Transplantation of MiR-28-5p-Modified BMSCs Promotes Functional Recovery After Spinal Cord Injury

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination

CCR3 gene knockout inhibits proliferation, differentiation, and migration of eosinophils in allergic rhinitis model mice

Electrical stimulation induced structural 3D human engineered neural tissue with well-developed neuronal network and functional connectivity

Mast cell stabilizer disodium cromoglycate improves long-term cognitive impairment after general anesthesia exposure in neonatal mice

JQ1 attenuates neuroinflammation by inhibiting the inflammasome-dependent canonical pyroptosis pathway in SAE

Dexpramipexole Attenuates White Matter Injury to Facilitate Locomotion and Motor Coordination Recovery via Reducing Ferroptosis after Intracerebral Hemorrhage

D-4F, an apolipoprotein A-I mimetic, promotes the clearance of myelin debris and the reduction of foamy macrophages after spinal cord injury

NEP1‑40 promotes myelin regeneration via upregulation of GAP‑43 and MAP‑2 expression after focal cerebral ischemia in rats Corrigendum in /10.3892/mmr.2023.12945

The Shh/Gli1 signaling pathway regulates regeneration via transcription factor Olig1 expression after focal cerebral ischemia in rats

[Met5]-enkephalin preserves diffusion metrics in EAE mice

Prox1 induces new lymphatic vessel formation and promotes nerve reconstruction in a mouse model of sciatic nerve crush injury

TrkA regulates the regenerative capacity of bone marrow stromal stem cells in nerve grafts

Effects of the transcription factor Olig1 on the differentiation and remyelination of oligodendrocyte precursor cells after focal cerebral ischemia in rats

Construction of Biofunctionalized Anisotropic Hydrogel Micropatterns and Their Effect on Schwann Cell Behavior in Peripheral Nerve Regeneration

Clobetasol propionate enhances neural stem cell and oligodendrocyte differentiation

Invasion of white matter tracts by glioma stem cells is regulated by a NOTCH1–SOX2 positive-feedback loop

Phenelzine, a small organic compound mimicking the functions of cell adhesion molecule L1, promotes functional recovery after mouse spinal cord injury

Procyanidin B2 mitigates behavioral impairment and protects myelin integrity in cuprizone-induced schizophrenia in mice

Phenelzine, a cell adhesion molecule L1 mimetic small organic compound, promotes functional recovery and axonal regrowth in spinal cord-injured zebrafish

Nexilin Regulates Oligodendrocyte Progenitor Cell Migration and Remyelination and Is Negatively Regulated by Protease-Activated Receptor 1/Ras-Proximate-1 Signaling Following Subarachnoid Hemorrhage

Neuroprotective effect of ischemic postconditioning on sciatic nerve transection

The human natural killer-1 (HNK-1) glycan mimetic ursolic acid promotes functional recovery after spinal cord injury in mouse

N-acetylcysteine attenuates the cuprizone-induced behavioral changes and oligodendrocyte loss in male C57BL/7 mice via its anti-inflammation actions

Inconsistence between number and function of autoreactive T cells in the course of experimental autoimmune encephalomyelitis

Nerve growth factor pretreatment inhibits lidocaine‑induced myelin damage via increasing BDNF expression and inhibiting p38 mitogen activation in the rat spinal cord

A Small Organic Compound Mimicking the L1 Cell Adhesion Molecule Promotes Functional Recovery after Spinal Cord Injury in Zebrafish

Erythropoietin reduces white matter damage in two-day-old rats exposed to hypoxic/ischemia injury

Myricetin alleviates cuprizone-induced behavioral dysfunction and demyelination in mice by Nrf2 pathway

Cyclin-dependent kinase inhibitor flavopiridol promotes remyelination in a cuprizone induced demyelination model

ROCK inhibition with fasudil promotes early functional recovery of spinal cord injury in rats by enhancing microglia phagocytosis

Changes in proinflammatory cytokines and white matter in chronically stressed rats

Focal MMP-2 and MMP-9 Activity at the Blood-Brain Barrier Promotes Chemokine-Induced Leukocyte Migration

Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury

Soluble epoxide hydrolase inhibition provides multi-target therapeutic effects in rats after spinal cord injury

Synergistic effects of Buyang Huanwu decoction and embryonic neural stem cell transplantation on the recovery of neurological function in a rat model of spinal cord injury

β-elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells

Molecular Alterations in the Hippocampus after Experimental Subarachnoid Hemorrhage

Apolipoprotein E and its mimetic peptide suppress Th1 and Th17 responses in experimental autoimmune encephalomyelitis

Cancer stem cell labeling using poly(l-lysine)-modified iron oxide nanoparticles

Brain histological changes in young mice submitted to diets with different ratios of n-6/n-3 polyunsaturated fatty acids during maternal pregnancy and lactation

The chemokine CXCL12 and its receptor CXCR4 promote glioma stem cell-mediated VEGF production and tumour angiogenesis via PI3K/AKT signalling

The interaction of Nogo-66 receptor with Nogo-p4 inhibits the neuronal differentiation of neural stem cells

Isolation and characterization of stem cell-like precursor cells from primary human anaplastic oligoastrocytoma

Review