SKU PA1050
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-Myelin Basic Protein/MBP Antibody
SKU/Catalog Number PA1050
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Myelin basic protein(MBP) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-Myelin Basic Protein/MBP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1050)
Host Rabbit
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein(182-197aa KLGGRDSRSGSPMARR), identical to the related rat and mouse sequences.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

/antibody/pa1050 1 WB anti myelin basic protein antibody.jpg

Anti-Myelin Basic Protein antibody, PA1050, Western blotting
WB: Mouse Brain Tissue Lysate

/antibody/pa1050 2 IHC anti myelin basic protein antibody.jpg

Anti-Myelin Basic Protein antibody, PA1050, IHC(P)
IHC(P): Rat Brain Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P02686
Gene Name MBP
Protein Name Myelin basic protein
Tissue Specificity MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. .
Alternative Names Myelin basic protein;MBP;Myelin A1 protein;Myelin membrane encephalitogenic protein;MBP;
Subcellular Localization Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic side of myelin.
Molecular Weight 33117 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. .
Background Myelin basic protein(MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.

Order Product (PA1050)

Promotion:

Buy primary get secondary antibody for free.

$50 fee for conjugation. Antibody size is reduced to 50ug.

Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280

USD $280

Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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Publications

Changes in proinflammatory cytokines and white matter in chronically stressed rats
Cyclin-dependent kinase inhibitor flavopiridol promotes remyelination in a cuprizone induced demyelination model
Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro
Synergistic effects of Buyang Huanwu decoction and embryonic neural stem cell transplantation on the recovery of neurological function in a rat model of spinal cord injury
Nogo-66 Promotes the Differentiation of Neural Progenitors into Astroglial Lineage Cells through mTOR-STAT3 Pathway
Zhou XB, Liu N, Wang D, Zou DX, Wei CW, Zhou JL. Neural Regen Res. 2018 Mar;13(3):492-496. doi: 10.4103/1673-5374.228733. Neuroprotective effect of ischemic postconditioning on sciatic nerve transection
Gao S, Zheng Y, Cai Q, Yao W, Wang J, Zhang P, Wang X. J Mater Sci Mater Med. 2014 May;25(5):1283-91. Doi: 10.1007/S10856-014-5150-3. Epub 2014 Jan 23. Comparison Of Morphology And Biocompatibility Of Acellular Nerve Scaffolds Processed By Differe...
Wang B, Xiao Z, Chen B, Han J, Gao Y, Zhang J, Zhao W, Wang X, Dai J. Plos One. 2008 Mar 26;3(3):E1856. Doi: 10.1371/Journal.Pone.0001856. Nogo-66 Promotes The Differentiation Of Neural Progenitors Into Astroglial Lineage Cells Through Mtor-Stat3 ...
Jin Sj, Liu Y, Deng Sh, Liao Lh, Lin Tl, Ning Q, Luo Xp. Int J Mol Med. 2015 May;35(5):1199-212. Doi: 10.3892/Ijmm.2015.2136. Epub 2015 Mar 13. Neuroprotective Effects Of Activated Protein C On Intrauterine Inflammation-Induced Neonatal White Matt...
Wei J, Zheng M, Liang P, Wei Y, Yin X, Tang Y, Xue Y. Neurobiol Dis. 2013 Aug;56:59-65. Doi: 10.1016/J.Nbd.2013.04.009. Epub 2013 Apr 22. Apolipoprotein E And Its Mimetic Peptide Suppress Th1 And Th17 Responses In Experimental Autoimmune Encephalo...
Chen X,, Chen X, Huang X, Qin C, Fang Y, Liu Y, Zhang G, Pan D, Wang W, Xie M. Mol Neurobiol. 2015 Feb 10. [Epub Ahead Of Print] Soluble Epoxide Hydrolase Inhibition Provides Multi-Target Therapeutic Effects In Rats After Spinal Cord Injury.
Han Sm, Wan H, Kudo G, Foltz Wd, Vines Dc, Green De, Zoerle T, Tariq A, Brathwaite S, D'Abbondanza J, Ai J, Macdonald Rl. J Cereb Blood Flow Metab. 2014 Jan;34(1):108-17. Doi: 10.1038/Jcbfm.2013.170. Epub 2013 Sep 25. Molecular Alterations In The ...
Wang X, Tu Q, Zhao B, An Y, Wang Jc, Liu W, Yuan Ms, Ahmed Sm, Xu J, Liu R, Zhang Y, Wang J. Biomaterials. 2013 Jan;34(4):1155-69. Doi: 10.1016/J.Biomaterials.2012.10.063. Epub 2012 Nov 17. Effects Of Poly(L-Lysine)-Modified Fe3O4 Nanoparticles On...
Ping Yf, Yao Xh, Jiang Jy, Zhao Lt, Yu Sc, Jiang T, Lin Mc, Chen Jh, Wang B, Zhang R, Cui Yh, Qian C, Wang Jm, Bian Xw. J Pathol. 2011 Jul;224(3):344-54. Doi: 10.1002/Path.2908. Epub 2011 May 27. The Chemokine Cxcl12 And Its Receptor Cxcr4 Promote...

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to mbp antibody, myelin basic protein antibody, sp antibody