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Product Info Summary
| SKU: | A01710-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Ghrelin GHRL Antibody
SKU/Catalog Number
A01710-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Ghrelin GHRL Antibody catalog # A01710-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ghrelin GHRL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01710-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Ghrelin. AA range:47-96
Reactive Species
A01710-1 is reactive to GHRL in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
12.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01710-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:100-300
Validation Images & Assay Conditions
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Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min).3, Picture B: DAPI (blue) 10min.
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Western Blot analysis of 293 cells using Ghrelin Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunohistochemistry analysis of Ghrelin antibody in paraffin-embedded human lung carcinoma tissue.
Specific Publications For Anti-Ghrelin GHRL Antibody (A01710-1)
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Customer Reviews
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3 Customer Q&As for Anti-Ghrelin GHRL Antibody
Question
Would A01710-1 anti-Ghrelin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
As indicated on the product datasheet, A01710-1 anti-Ghrelin antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-29
Question
Would anti-Ghrelin antibody A01710-1 work for WB with placenta stomach?
A. Parker
Verified customer
Asked: 2018-11-13
Answer
According to the expression profile of placenta stomach, GHRL is highly expressed in placenta stomach. So, it is likely that anti-Ghrelin antibody A01710-1 will work for WB with placenta stomach.
Boster Scientific Support
Answered: 2018-11-13
Question
We are currently using anti-Ghrelin antibody A01710-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
F. Krishna
Verified customer
Asked: 2013-11-18
Answer
The anti-Ghrelin antibody (A01710-1) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-18
