Human Haptoglobin ELISA Kit PicoKine™

Human Haptoglobin ELISA Kit PicoKine™ (96 Tests). Quantitate Human HP in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml . Cited in 2 publication(s).

Product Info Summary

SKU: EK1702
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human Haptoglobin ELISA Kit PicoKine™

See all Haptoglobin products

SKU/Catalog Number

EK1702

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Haptoglobin ELISA Kit PicoKine™ (96 Tests). Quantitate Human HP in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml .

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Haptoglobin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1702)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: V19-N406

Sensitivity

<20 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1702 is reactive to HP in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1702 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1702-CAP Anti-Human HP Pre-coated 96-well strip microplate 1
EK1702-ST Human HP Standard 2 vials, 200 ng/tube
EK1702-DA Human HP Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0700.3320.5260.9981.3291.7201.9612.389

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Haptoglobin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7782764990077428959099
Standard deviation37.34110.56712.839.47167.91718.82
CV (%)4.8%4%7.2%7.9%5.8%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Haptoglobin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 177869880478176540.15.2%
Sample 22764269727052507266896.623.6%
Sample 3990010211101141044110166194.361.9%
*number of samples for each test n=16.

Gene/Protein Information For HP (Source: Uniprot.Org, NCBI)

Uniprot ID

P00738

Gene ID

3240

Gene Name

HP

Full Name

Haptoglobin

Weight

45.205kDa

Superfamily

peptidase S1 family

Alternative Names

binding peptide; BP; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; Haptoglobin; haptoglobin, alpha polypeptide; haptoglobin, beta polypeptide; HP; HP-1; hp2-alpha; HP2ALPHA2; HPA1S; MGC111141; zonulin HP BP2ALPHA2, HPA1S, HP haptoglobin haptoglobin|binding peptide|haptoglobin alpha(1S)-beta|haptoglobin alpha(2FS)-beta|haptoglobin, alpha polypeptide|haptoglobin, beta polypeptide|zonulin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HP, check out the HP Infographic

HP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1702 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of haptoglobin in human keratinocytes and Langerhans cells

Localization of haptoglobin in normal human skin and some skin diseases

Have you used Human Haptoglobin ELISA Kit PicoKine™?

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8 Customer Q&As for Human Haptoglobin ELISA Kit PicoKine™

Question

Can EK1702 recognize Hp1-1, Hp2-1, and Hp2-2?

Verified customer

Asked: 2020-10-13

Answer

The Human Haptoglobin ELISA Kit PicoKine™ (EK1702) recognizes natural human Haptoglobin. It also recognizes natural human Haptoglobin 1-1 and Haptoglobin 2-2.

Boster Scientific Support

Answered: 2020-10-13

Question

What is the suggested dilution ratio of serum sample for EK1702?

Verified customer

Asked: 2020-10-07

Answer

For the Human Haptoglobin ELISA Kit PicoKine™ (EK1702), the suggested dilution ratio of serum sample is 1:1000000.

Boster Scientific Support

Answered: 2020-10-09

Question

Q: can you recommend the dilution ratio of serum samples for detection of Haptoglobin in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

W. Puri

Verified customer

Asked: 2019-12-29

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Haptoglobin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-12-29

Question

Q: how can I thaw whole blood sample for Haptoglobin ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Haptoglobin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-12-06

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

W. Thompson

Verified customer

Asked: 2019-07-28

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-07-28

Question

Q: if the enzyme conjugated Haptoglobin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Haptoglobin antigen?

Verified Customer

Verified customer

Asked: 2018-10-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-10-25

Question

Q: Can Haptoglobin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2017-05-15

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-05-15

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

E. Kaur

Verified customer

Asked: 2015-03-27

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2015-03-27

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