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GPX4 antibody (M02059-1) performs well in Flow Cytometry, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of Hela cells using anti-Glutathione Peroxidase 4/GPX4 antibody (M02059-1).
Overlay histogram showing Hela cells stained with M02059-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Glutathione Peroxidase 4/GPX4 Antibody (M02059-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Hela cells stained with M02059-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Glutathione Peroxidase 4/GPX4 Antibody (M02059-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7)
GPX4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, WB |
Tested Applications
GPX4 antibody was positively detected by WB in:
human HepG2 whole cell, human CACO-2 whole cell, human 293T whole cell, human K562 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GPX4 antibody was positively detected by FC in:
Hela cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
Human
Tested
Monkey
Predicted
Mouse
Predicted
Bovine
Predicted
Sheep
Predicted
Dog
Predicted
Rat
Predicted
Product Information
Product Name
Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7)
View all GPX4 Antibodies
View all GPX4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
6I4E7
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
19 kDa
Calculated Molecular Weight
22.2 kDa
Background of GPX4
Glutathione peroxidase 4, also known as GPX4, is an enzyme that in humans is encoded by the GPX4 gene. This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02059-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Mouse
93%
High Homology
Cow
93%
High Homology
Sheep
93%
High Homology
Dog
92%
High Homology
Rat
92%
High Homology
Zebrafish
73%
Moderate Homology
Drosophila
45%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GPX4 antibody
Flow Cytometry (FC) protocol for GPX4 antibody
Publications
Application:
Species:
SIRT5 safeguards against T-2 toxin induced liver injury by repressing iron accumulation, oxidative stress, and the activation of NLRP3 inflammasome
Published: 2024-09-04
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY
Application: WB
Impact Factor: 3.30
Species: Mouse
PMID: 39241930
Contribution of impaired autophagy, mitochondrial dysfunction and abnormal lipolysis to epididymal aging in mice
Published: 2024-07-26
Journal: EXPERIMENTAL GERONTOLOGY
Application: WB
Impact Factor: 3.30
Species: Mouse
PMID: 39067197
Expression of the laminin genes family and its relationship to prognosis in pancreatic carcinoma
Published: 2024-07-21
Journal: Arab Journal of Gastroenterology
Application: WB
Impact Factor: 1.10
Species: Human
PMID: 39039002
Review
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Product Promise
We promise Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7) performs excellently in relevant applications for Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M02059-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026