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Product Info Summary
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Anti-Nebulin Antibody (Monoclonal, NB2)
Boster Bio Anti-Nebulin Antibody (Monoclonal, NB2) catalog # MA1069. Tested in WB applications. This antibody reacts with Chicken.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nebulin Antibody (Monoclonal, NB2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1069)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Chicken breast nebulin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
MA1069 is reactive to NEB in Chicken
MA1069 is guaranteed for WB Boster Guarantee
Background of Nebulin
Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 1-2 μg/ml, Chicken
Validation Images & Assay Conditions
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Anti-Nebulin antibody, MA1069, Western blotting
All lanes: Anti Nebulin (MA1069) at 0.5ug/ml
WB: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 773KD
Observed bind size: 773KD
Gene/Protein Information For NEB (Source: Uniprot.org, NCBI)
DKFZp686C1456; FLJ11505; FLJ36536; FLJ39568; FLJ39584; NEB177D; nebulin; NEM2 NEB NEB177D, NEM2 nebulin nebulin|nemaline myopathy type 2*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on NEB, check out the NEB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
MA1069 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li X, Ni M, Zhang C, Ma W, Zhang Y. Rna. 2014 Feb;20(2):252-9. Doi: 10.1261/Rna.040220.113. Epub 2013 Dec 17. A Convenient System For Highly Specific And Sensitive Detection Of Mirna Expression.
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