|Ig Isotype||Mouse IgG1|
|Product Name||Anti-Nebulin Antibody (Monoclonal, NB2)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Nebulin, nebulin (NEB) detection. Tested with WB in chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Nebulin Antibody (Monoclonal, NB2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1069)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Chicken breast nebulin.|
Assay Dilutions Overview
Western blot, 1-2 μg/ml, Chicken
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
Images And Assay Conditions
Anti- Nebulin antibody, MA1069, Western blotting
All lanes: Anti Nebulin (MA1069) at 0.5ug/ml
WB: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 773KD
Observed bind size: 773KD
Protein Target Info (Source: Uniprot.org)
|Tissue Specificity||Muscle specific. Located in the thin filament of striated muscle.|
|Subcellular Localization||Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.|
|Molecular Weight||772914 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.|
|Research Areas||Actin Binding Proteins, Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction
*You can search these to find other products in these research areas.
|Background||Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.|
Other Recommended Resources
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