Anti-Nebulin Antibody (Monoclonal, NB2)

Nebulin antibody

Boster Bio Anti-Nebulin Antibody (Monoclonal, NB2) catalog # MA1069. Tested in WB applications. This antibody reacts with Chicken. Cited in 1 publication(s).

Product Info Summary

SKU: MA1069
Size: 100μg/vial
Reactive Species: Chicken
Host: Mouse
Application: WB

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Product Name

Anti-Nebulin Antibody (Monoclonal, NB2)

View all Nebulin Antibodies

SKU/Catalog Number

MA1069

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Nebulin Antibody (Monoclonal, NB2) catalog # MA1069. Tested in WB applications. This antibody reacts with Chicken.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nebulin Antibody (Monoclonal, NB2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1069)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

NB2

Isotype

Mouse IgG1

Immunogen

Chicken breast nebulin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1069 is reactive to NEB in Chicken

Applications

MA1069 is guaranteed for WB Boster Guarantee

Background of Nebulin

Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 1-2 μg/ml, Chicken

Validation Images & Assay Conditions

Gene/Protein Information For NEB (Source: Uniprot.Org, NCBI)

Uniprot ID

P20929

Gene ID

4703

Gene Name

NEB

Full Name

Nebulin

Weight

772.914kDa

Alternative Names

DKFZp686C1456; FLJ11505; FLJ36536; FLJ39568; FLJ39584; NEB177D; nebulin; NEM2 NEB NEB177D, NEM2 nebulin nebulin|nemaline myopathy type 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NEB, check out the NEB Infographic

NEB infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NEB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1069 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li X, Ni M, Zhang C, Ma W, Zhang Y. Rna. 2014 Feb;20(2):252-9. Doi: 10.1261/Rna.040220.113. Epub 2013 Dec 17. A Convenient System For Highly Specific And Sensitive Detection Of Mirna Expression.

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2 Customer Q&As for Anti-Nebulin Antibody (Monoclonal, NB2)

Question

We are currently using anti-Nebulin antibody (Monoclonal, NB2) MA1069 for chicken tissue, and we are content with the WB results. The species of reactivity given in the datasheet says chicken. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

The anti-Nebulin antibody (Monoclonal, NB2) (MA1069) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-04

Question

We are interested in using your anti-Nebulin antibody (Monoclonal, NB2) for regulation of actin filament length studies. Has this antibody been tested with western blotting on mouse skeletal muscle tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-09-06

Answer

We appreciate your inquiry. This MA1069 anti-Nebulin antibody (Monoclonal, NB2) is validated on mouse skeletal muscle tissue, tissue lysate. It is guaranteed to work for WB in chicken. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-09-06

Size

Total: $315

SKU:MA1069

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MA1069
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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