Anti-Nebulin Antibody (Monoclonal, NB2)

SKU MA1069
Size 100μg/vial
Reactivity Chicken
Clonality Monoclonal
Clone Number NB2
Host Mouse
Ig Isotype Mouse IgG1
Applications WB

Overview

Product Name Anti-Nebulin Antibody (Monoclonal, NB2)
SKU/Catalog Number MA1069
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Nebulin, nebulin (NEB) detection. Tested with WB in chicken. No cross reactivity with other proteins.
Cite This Product Anti-Nebulin Antibody (Monoclonal, NB2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1069)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Chicken breast nebulin.
Reactivity Chicken

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Western blot, 1-2 μg/ml, Chicken

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Images And Assay Conditions

Anti- Nebulin antibody, MA1069, Western blotting
All lanes: Anti Nebulin (MA1069) at 0.5ug/ml
WB: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 773KD
Observed bind size: 773KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P20929
Gene Name NEB
Protein Name Nebulin
Tissue Specificity Muscle specific. Located in the thin filament of striated muscle.
Alternative Names Nebulin;NEB;
Subcellular Localization Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.
Molecular Weight 772914 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.
Research Areas Chicken

*You can search these to find other products in these research areas.
Background Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.

Other Recommended Resources

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Monoclonal clone# G2 antibody for NEB detection. Host: Mouse.Size: 100μg/vial. Tested applications: WB. Reactive species: Chicken. NEB information: Molecular Weight: 772914 MW; Subcellular Localization: Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton; Tissue Specificity: Muscle specific. Located in the thin filament of striated muscle.
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In stock
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MA1069
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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Publications

Li X, Ni M, Zhang C, Ma W, Zhang Y. Rna. 2014 Feb;20(2):252-9. Doi: 10.1261/Rna.040220.113. Epub 2013 Dec 17. A Convenient System For Highly Specific And Sensitive Detection Of Mirna Expression.

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
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