radio
SKU MA1069
Size 100μg/vial
Reactivity Chicken
Clonality Monoclonal
Clone Number NB2
Host Mouse
Ig Isotype Mouse IgG1
Applications WB

Overview

Product Name Anti-Nebulin Antibody (Monoclonal, NB2)
SKU/Catalog Number MA1069
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Nebulin, nebulin (NEB) detection. Tested with WB in chicken. No cross reactivity with other proteins.
Cite This Product Anti-Nebulin Antibody (Monoclonal, NB2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1069)
Host Mouse
Immunogen Chicken breast nebulin.
Reactivity Chicken

Assay Details

Assay Dilutions Overview

Western blot, 1-2 μg/ml, Chicken

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Images And Assay Conditions

/antibody/ma1069 1 WB anti nebulin antibody monoclonal nb2.jpg

Anti- Nebulin antibody, MA1069, Western blotting
All lanes: Anti Nebulin (MA1069) at 0.5ug/ml
WB: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 773KD
Observed bind size: 773KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P20929
Gene Name NEB
Protein Name Nebulin
Tissue Specificity Muscle specific. Located in the thin filament of striated muscle.
Alternative Names Nebulin;NEB;
Subcellular Localization Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.
Molecular Weight 772914 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.
Background Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.

Order Product (MA1069)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240
Ships in 5-7 business days.

Troubleshooting

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Publications

Li X, Ni M, Zhang C, Ma W, Zhang Y. Rna. 2014 Feb;20(2):252-9. Doi: 10.1261/Rna.040220.113. Epub 2013 Dec 17. A Convenient System For Highly Specific And Sensitive Detection Of Mirna Expression.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.