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- Table of Contents
FFAR1 antibody (A01349-2) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Western blot analysis of GPCR GPR40 using anti-GPCR GPR40 antibody (A01349-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR GPR40 antigen affinity purified polyclonal antibody (Catalog # A01349-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR GPR40 at approximately 31KD. The expected band size for GPCR GPR40 is at 31KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR GPR40 antigen affinity purified polyclonal antibody (Catalog # A01349-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR GPR40 at approximately 31KD. The expected band size for GPCR GPR40 is at 31KD.
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Anti-GPCR GPR40/FFAR1 Antibody Picoband®
FFAR1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
FFAR1 antibody was positively detected by WB in:
human PANC-1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Human
Tested
Dog
Predicted
Monkey
Predicted
Sheep
Predicted
Product Information
Product Name
Anti-GPCR GPR40/FFAR1 Antibody Picoband®
View all FFAR1 Antibodies
View all FFAR1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GPCR GPR40, which shares 88% and 84% amino acid (aa) sequence identity with mouse and rat GPCR GPR40, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
31 kDa
Calculated Molecular Weight
31.5 kDa
Background of FFAR1
Free fatty acid receptor 1 (FFA1), also known as GPR40, is a class A G-protein coupled receptor that in humans is encoded by the FFAR1 gene. This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes.
Cite This Product
Anti-GPCR GPR40/FFAR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01349-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
96%
High Homology
Monkey
96%
High Homology
Sheep
92%
High Homology
Mouse
88%
Moderate Homology
Rat
84%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FFAR1 antibody
Publications
Application:
Species:
Palmitic Acid Methyl Ester Enhances Adipogenic Differentiation in Rat Adipose Tissue-Derived Mesenchymal Stem Cells through a G Protein-Coupled Receptor-Mediated Pathway
Published: 2021-10-05
Journal: Stem Cells International
Application: WB
Impact Factor: 5.44
Species: Rat
PMID: 34650609
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Product Promise
We promise Anti-GPCR GPR40/FFAR1 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01349-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026