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Product Info Summary
| SKU: | A01349-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GPCR GPR40/FFAR1 Antibody Picoband®
SKU/Catalog Number
A01349-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GPCR GPR40/FFAR1 Antibody Picoband® catalog # A01349-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPCR GPR40/FFAR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01349-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GPCR GPR40, which shares 88% and 84% amino acid (aa) sequence identity with mouse and rat GPCR GPR40, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01349-2 is reactive to FFAR1 in Human, Mouse, Rat
Observed Molecular Weight
31 kDa
Calculated molecular weight
31.5 kDa
Background of FFAR1
Free fatty acid receptor 1 (FFA1), also known as GPR40, is a class A G-protein coupled receptor that in humans is encoded by the FFAR1 gene. This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01349-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human PANC-1 whole cell
Validation Images & Assay Conditions
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Western blot analysis of GPCR GPR40 using anti-GPCR GPR40 antibody (A01349-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR GPR40 antigen affinity purified polyclonal antibody (Catalog # A01349-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR GPR40 at approximately 31KD. The expected band size for GPCR GPR40 is at 31KD.
Specific Publications For Anti-GPCR GPR40/FFAR1 Antibody Picoband® (A01349-2)
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Customer Q&As
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7 Customer Q&As for Anti-GPCR GPR40/FFAR1 Antibody Picoband®
Question
Would anti-GPCR GPR40/FFAR1 antibody A01349-2 work on bovine WB with right ovary?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
Our lab technicians have not tested anti-GPCR GPR40/FFAR1 antibody A01349-2 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-GPCR GPR40/FFAR1 antibody A01349-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine right ovary in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-05
Question
I have a question about product A01349-2, anti-GPCR GPR40/FFAR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Anderson
Verified customer
Asked: 2019-10-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01349-2 anti-GPCR GPR40/FFAR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-07
Question
Will A01349-2 anti-GPCR GPR40/FFAR1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
You can see on the product datasheet, A01349-2 anti-GPCR GPR40/FFAR1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-31
Question
I was wanting to use your anti-GPCR GPR40/FFAR1 antibody for WB for human right ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human right ovary identification?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
As indicated on the product datasheet, A01349-2 anti-GPCR GPR40/FFAR1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human right ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-13
Question
Please see the WB image, lot number and protocol we used for right ovary using anti-GPCR GPR40/FFAR1 antibody A01349-2. Please let me know if you require anything else.
H. Moore
Verified customer
Asked: 2017-12-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right ovary using anti-GPCR GPR40/FFAR1 antibody A01349-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-18
Question
We are currently using anti-GPCR GPR40/FFAR1 antibody A01349-2 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Z. Gonzalez
Verified customer
Asked: 2013-06-20
Answer
The anti-GPCR GPR40/FFAR1 antibody (A01349-2) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-20
