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Product Info Summary
| SKU: | A30790 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-GPR109 HCAR3 Antibody
SKU/Catalog Number
A30790
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GPR109 HCAR3 Antibody (Catalog# A30790). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR109 HCAR3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30790)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GPR109. Immunogen sequence location: 285 - 334
Reactive Species
A30790 is reactive to HCAR3 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
44.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30790 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of MCF7 cells, using GPR109 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from RAW264.7 cells, using GPR109 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HepG2 cells using GPR109 antibody.
Specific Publications For Anti-GPR109 HCAR3 Antibody (A30790)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-GPR109 HCAR3 Antibody
Question
Is a blocking peptide available for product anti-GPR109 antibody (A30790)?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
We do provide the blocking peptide for product anti-GPR109 antibody (A30790). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-27
Question
I have a question about product A30790, anti-GPR109 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30790 anti-GPR109 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-16
Question
We are currently using anti-GPR109 antibody A30790 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
S. Mangal
Verified customer
Asked: 2016-04-13
Answer
The anti-GPR109 antibody (A30790) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-04-13
Question
I am interested in to test anti-GPR109 antibody A30790 on rat pancreas for research purposes, then I may be interested in using anti-GPR109 antibody A30790 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Huang
Verified customer
Asked: 2015-08-05
Answer
The products we sell, including anti-GPR109 antibody A30790, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-08-05
