Click image to see more details
Product Info Summary
| SKU: | A30807 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-GPR139 Antibody
SKU/Catalog Number
A30807
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GPR139 Antibody (Catalog# A30807). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR139 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30807)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GPR139. Immunogen sequence location: 181 - 230
Reactive Species
A30807 is reactive to GPR139 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
40.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30807 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of K562 cells using GPR139 Polyclonal Antibody
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Immunofluorescence analysis of LOVO cells, using GPR139 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR139 Antibody (A30807)
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Customer Reviews
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3 Customer Q&As for Anti-GPR139 Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for caudate nucleus using anti-GPR139 antibody A30807. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-03
Question
We are currently using anti-GPR139 antibody A30807 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
B. Anderson
Verified customer
Asked: 2019-06-10
Answer
The anti-GPR139 antibody (A30807) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-10
Question
Would anti-GPR139 antibody A30807 work on pig IF with caudate nucleus?
R. Carter
Verified customer
Asked: 2018-04-10
Answer
Our lab technicians have not tested anti-GPR139 antibody A30807 on pig. You can run a BLAST between pig and the immunogen sequence of anti-GPR139 antibody A30807 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig caudate nucleus in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-10
