Anti-GPR133 Antibody

GPR133 antibody

Boster Bio Anti-GPR133 Antibody (Catalog# A30805). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Monkey.

Product Info Summary

SKU: A30805
Size: 100ul
Reactive Species: Human, Monkey
Host: Rabbit
Application: ELISA, IF, ICC, WB

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Product Name

Anti-GPR133 Antibody

View all GPR133 Antibodies

SKU/Catalog Number

A30805

Size

100ul

Form

Liquid

Description

Boster Bio Anti-GPR133 Antibody (Catalog# A30805). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Monkey.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GPR133 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30805)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human GPR133. Immunogen sequence location: 461 - 510

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30805 is reactive to Adgrd1 in Human, Monkey

Applications

A30805 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

99.277kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For Adgrd1 (Source: Uniprot.org, NCBI)

Gene Name

Adgrd1

Full Name

Adhesion G-protein coupled receptor D1

Weight

99.277kDa

Superfamily

G-protein coupled receptor 2 family

Alternative Names

DKFZp434B1272; FLJ16770; G protein-coupled receptor 133; GPR133; G-protein coupled receptor PGR25; MGC138514; PGR25; PGR25MGC138512; probable G-protein coupled receptor 133 Adgrd1|E230012M21Rik, Gpr1, Gpr133|adhesion G protein-coupled receptor D1|adhesion G-protein coupled receptor D1|G protein coupled receptor Gpr133|G-protein coupled receptor PGR25|probable G-protein coupled receptor 133

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Adgrd1, check out the Adgrd1 Infographic

Adgrd1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Adgrd1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-GPR133 Antibody

Question

I was wanting to use your anti-GPR133 antibody for IF for human right atrium auricular region on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right atrium auricular region identification?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

As indicated on the product datasheet, A30805 anti-GPR133 antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right atrium auricular region in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-25

Question

We are currently using anti-GPR133 antibody A30805 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

The anti-GPR133 antibody (A30805) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-02

Question

Does A30805 anti-GPR133 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-19

Answer

You can see on the product datasheet, A30805 anti-GPR133 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-19

Question

Is a blocking peptide available for product anti-GPR133 antibody (A30805)?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

We do provide the blocking peptide for product anti-GPR133 antibody (A30805). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-10

Size

Total: $335

SKU:A30805

Backordered.

Lead time for this item is typically 5-7 days

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A30805
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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