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Product Info Summary
| SKU: | A30805 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-GPR133 Antibody
SKU/Catalog Number
A30805
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GPR133 Antibody (Catalog# A30805). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR133 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30805)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GPR133. Immunogen sequence location: 461 - 510
Reactive Species
A30805 is reactive to ADGRD1 in Human, Monkey
Observed Molecular Weight
72 kDa
Calculated molecular weight
96.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30805 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HeLa cells, using GPR133 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COS7 cells, using GPR133 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from K562 cells using GPR133 antibody.
Specific Publications For Anti-GPR133 Antibody (A30805)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-GPR133 Antibody
Question
I was wanting to use your anti-GPR133 antibody for IF for human right atrium auricular region on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right atrium auricular region identification?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
As indicated on the product datasheet, A30805 anti-GPR133 antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right atrium auricular region in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-25
Question
We are currently using anti-GPR133 antibody A30805 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
The anti-GPR133 antibody (A30805) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-02
Question
Does A30805 anti-GPR133 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
You can see on the product datasheet, A30805 anti-GPR133 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-19
Question
Is a blocking peptide available for product anti-GPR133 antibody (A30805)?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
We do provide the blocking peptide for product anti-GPR133 antibody (A30805). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-10
