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Product Info Summary
| SKU: | A30812 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GPR149 Antibody
SKU/Catalog Number
A30812
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GPR149 Antibody (Catalog# A30812). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR149 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30812)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GPR149. Immunogen sequence location: 451 - 500
Reactive Species
A30812 is reactive to GPR149 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
81.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30812 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HeLa cells, using GPR149 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HeLa and COLO205 cells, using GPR149 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from 293 cells using GPR149 antibody.
Specific Publications For Anti-GPR149 Antibody (A30812)
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Customer Reviews
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5 Customer Q&As for Anti-GPR149 Antibody
Question
We are currently using anti-GPR149 antibody A30812 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The anti-GPR149 antibody (A30812) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-25
Question
Will A30812 anti-GPR149 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
It shows on the product datasheet, A30812 anti-GPR149 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-26
Question
Is there a BSA free version of anti-GPR149 antibody A30812 available?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GPR149 antibody A30812 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-25
Question
Is this A30812 anti-GPR149 antibody reactive to the isotypes of GPR149?
S. Zhao
Verified customer
Asked: 2019-11-13
Answer
The immunogen of A30812 anti-GPR149 antibody is A peptide derived from human GPR149. Immunogen sequence location: 451 - 500. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-13
Question
I have a question about product A30812, anti-GPR149 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Gonzalez
Verified customer
Asked: 2015-09-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30812 anti-GPR149 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-09-21
