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Product Info Summary
| SKU: | A02357 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MAD2L2/Mad2B Antibody
SKU/Catalog Number
A02357
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-MAD2L2/Mad2B Antibody (Catalog # A02357). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-MAD2L2/Mad2B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02357)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-terminus region of Human MAD2L2.
Reactive Species
A02357 is reactive to MAD2L2 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
24.3 kDa
Background of MAD2L2
MAD2L2 is also known as MAD2B, REV7 and mitotic arrest deficient-like 2 protein. MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02357 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:7,500 - 1:30,000
IHC: 1:500 - 1:2,000
WB: 1:500 - 1:2,000
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. For western blotting HeLa whole cell lysates and nuclear extracts are suggested. Reactivity in other immunoassays is unknown. Antibody activity can be blocked using a control peptide (000-001-470).
Validation Images & Assay Conditions
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Boster's Affinity Purified anti-MAD2L2 antibody shows strong nuclear and cytoplasmic staining of tumor cells in cancerous human kidney tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
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Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Lane 1: HeLa whole cell lysate and nuclear lysate were probed using this antibody. This antibody clearly detects a ~20 kDa band corresponding to human MAD2L2 (predicted molecular weight is 24 kDa). Approximately 20 µg of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.
Specific Publications For Anti-MAD2L2/Mad2B Antibody (A02357)
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