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Product Info Summary
| SKU: | A10245 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-GPR172B SLC52A1 Antibody
SKU/Catalog Number
A10245
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR172B SLC52A1 Antibody catalog # A10245. Tested in WB, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR172B SLC52A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10245)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PEVR2. AA range:235-284
Reactive Species
A10245 is reactive to SLC52A1 in Human
Calculated molecular weight
46.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10245 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of 293T cells using GPR172B Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from LOVO cells, using PEVR2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of MCF7 cells, using PEVR2 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR172B SLC52A1 Antibody (A10245)
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Customer Q&As
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6 Customer Q&As for Anti-GPR172B SLC52A1 Antibody
Question
Is a blocking peptide available for product anti-GPR172B antibody (A10245)?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
We do provide the blocking peptide for product anti-GPR172B antibody (A10245). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-06
Question
Would A10245 anti-GPR172B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
As indicated on the product datasheet, A10245 anti-GPR172B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-01
Question
Does anti-GPR172B antibody A10245 work on goat WB with placenta?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
Our lab technicians have not tested anti-GPR172B antibody A10245 on goat. You can run a BLAST between goat and the immunogen sequence of anti-GPR172B antibody A10245 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-26
Question
Would anti-GPR172B antibody A10245 work for WB with embryo?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
According to the expression profile of embryo, SLC52A1 is highly expressed in embryo. So, it is likely that anti-GPR172B antibody A10245 will work for WB with embryo.
Boster Scientific Support
Answered: 2019-07-25
Question
Is this A10245 anti-GPR172B antibody reactive to the isotypes of SLC52A1?
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
The immunogen of A10245 anti-GPR172B antibody is Synthesized peptide derived from the Internal region of human GPR172B. at AA rangle: 210-290. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-26
Question
I see that the anti-GPR172B antibody A10245 works with WB, what is the protocol used to produce the result images on the product page?
P. Zhang
Verified customer
Asked: 2014-03-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-03-24
