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Product Info Summary
| SKU: | A13599 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-GPR175 TPRA1 Antibody
SKU/Catalog Number
A13599
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR175 TPRA1 Antibody catalog # A13599. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR175 TPRA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13599)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR175. AA range:198-247
Reactive Species
A13599 is reactive to TPRA1 in Human, Mouse, Rat
Calculated molecular weight
41.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13599 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using GPR175 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from 293 cells, using GPR175 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR175 TPRA1 Antibody (A13599)
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Customer Q&As
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6 Customer Q&As for Anti-GPR175 TPRA1 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas skin using anti-GPR175 antibody A13599. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-09
Question
Does anti-GPR175 antibody A13599 work on dog WB with fetal brain?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Our lab technicians have not validated anti-GPR175 antibody A13599 on dog. You can run a BLAST between dog and the immunogen sequence of anti-GPR175 antibody A13599 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog fetal brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-22
Question
I have a question about product A13599, anti-GPR175 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13599 anti-GPR175 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-14
Question
Is this A13599 anti-GPR175 antibody reactive to the isotypes of TPRA1?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
The immunogen of A13599 anti-GPR175 antibody is Synthesized peptide derived from the Internal region of human GPR175. at AA rangle: 170-250. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-02
Question
My question regards to test anti-GPR175 antibody A13599 on rat pancreas skin for research purposes, then I may be interested in using anti-GPR175 antibody A13599 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
The products we sell, including anti-GPR175 antibody A13599, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-20
Question
I see that the anti-GPR175 antibody A13599 works with WB, what is the protocol used to produce the result images on the product page?
E. Zhang
Verified customer
Asked: 2013-02-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-02-06
