Product Info Summary
| SKU: | PA1977 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-VRL1/TRPV2 Antibody Picoband®
SKU/Catalog Number
PA1977
BA4959 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VRL1/TRPV2 Antibody catalog # PA1977. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VRL1/TRPV2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1977)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human VRL1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1977 is reactive to TRPV2 in Human
Observed Molecular Weight
450 kDa
Calculated molecular weight
86.0 kDa
Background of TRPV2
TRPV2 (Transient Receptor Potential Cation Channel Subfamily V Member 2), also known as VRL1, is a protein that, in humans, is encoded by the TRPV1 gene. The International Radiation Hybrid Mapping Consortium mapped the TRPV2 gene to chromosome 17. This gene encodes an ion channel that is activated by high temperatures above 52ËšC. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is though that in other tissues the channel may be activated by stimuli other than heat.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1977 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Cell
IHC: Human Mammary Cancer Tissue, Human Intestinal Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-VRL1 antibody, PA1977, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-VRL1 antibody, PA1977, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Click image to see more details
Anti-VRL1 antibody, PA1977, Western blotting
WB: HELA Cell Lysate
Specific Publications For Anti-VRL1/TRPV2 Antibody Picoband® (PA1977)
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1 Customer Q&As for Anti-VRL1/TRPV2 Antibody Picoband®
Question
We are currently using anti-VRL1/TRPV2 antibody PA1977 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
The anti-VRL1/TRPV2 antibody (PA1977) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-01


