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Product Info Summary
| SKU: | A05578 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-GPR56 ADGRG1 Antibody
SKU/Catalog Number
A05578
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR56 ADGRG1 Antibody catalog # A05578. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR56 ADGRG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05578)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR56. AA range:251-300
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05578 is reactive to ADGRG1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
77.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05578 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of NIH-3T3 cells using GPR56 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from K562 cells, using GPR56 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of MCF7 cells, using GPR56 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR56 ADGRG1 Antibody (A05578)
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Customer Q&As
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6 Customer Q&As for Anti-GPR56 ADGRG1 Antibody
Question
I see that the anti-GPR56 antibody A05578 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adult mammalian kidney using anti-GPR56 antibody A05578. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-16
Question
Would A05578 anti-GPR56 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Edwards
Verified customer
Asked: 2019-08-19
Answer
It shows on the product datasheet, A05578 anti-GPR56 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-19
Question
Is a blocking peptide available for product anti-GPR56 antibody (A05578)?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
We do provide the blocking peptide for product anti-GPR56 antibody (A05578). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-02
Question
Would anti-GPR56 antibody A05578 work for WB with adult mammalian kidney?
Verified Customer
Verified customer
Asked: 2019-03-01
Answer
According to the expression profile of adult mammalian kidney, ADGRG1 is highly expressed in adult mammalian kidney. So, it is likely that anti-GPR56 antibody A05578 will work for WB with adult mammalian kidney.
Boster Scientific Support
Answered: 2019-03-01
Question
Is this A05578 anti-GPR56 antibody reactive to the isotypes of ADGRG1?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
The immunogen of A05578 anti-GPR56 antibody is Synthesized peptide derived from the Internal region of human GPR56. at AA rangle: 220-300. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-18
