Anti-Granzyme B/GZMB Antibody
Rabbit IgG polyclonal antibody for Granzyme B(GZMB) detection. Tested with WB in Human.
Anti-Granzyme B/GZMB Antibody Info At A Glance
|Product Name||Anti-Granzyme B/GZMB Antibody
See all Granzyme B primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Granzyme B/GZMB detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. Granzyme B/GZMB information: Molecular Weight: 27716 MW; Subcellular Localization: Cytoplasmic granule . Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.|
|Cite This Product||Anti-Granzyme B/GZMB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1738)|
|Similar Products From
|Anti-Granzyme B/GZMB Antibody may replace the following items: sc 1968|sc 1969|sc 56119|sc 73620|sc 8022.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Granzyme B(168-184aa QEDRKCESDLRHYYDST).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For GZMB (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3002|
|Species Of This Entry||Human|
|Protein Name||Granzyme B|
|Superfamily||peptidase S1 family|
|Alternative Names||Granzyme B|C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Glycosylation; Methylation; Reduction; Acetylation; Dephosphorylation; Myristoylation; Ubiquitination|
|Subcellular Localization||Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.|
|Gene Location||14q12, on Chromasome 14, gene sequence: NC_000014.9 (24630954..24634190, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For GZMB (Source: Uniprot.org, NCBI)
|Protein Function||This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.|
|Interacting Proteins||PRF1; SRGN|
|Related Diseases||Neoplasms; Malignant Neoplasms; Lymphoma; Virus Diseases; Carcinoma; Infective Disorder; Leukemia; T-cell Lymphoma; Autoimmune Reaction; Inflammation; Cytomegalovirus Infections; Autoimmune Diseases; Graft-vs-host Disease; Malignant Paraganglionic Neoplasm; Influenza; Decreased Immunologic Activity [pe]; Hiv Infections; Melanoma; Arthritis; Peripheral T-cell Lymphoma|
|Related Pathways||Cell Death; Immune Response; Exocytosis; Pathogenesis; Induction Of Apoptosis; Secretion; Localization; Cell Activation; Proteolysis; Cell Killing; Cytolysis; Programmed Cell Death; T Cell Activation; Cell Proliferation; Cytokine Production; Endocytosis; Cell Cycle; Cell Recognition; Cell Adhesion; Cell Differentiation|
|Background||Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes(CTL) and natural killer(NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are though to protect their host by inducing apoptosis of cells that bear on their surface "nonself" antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.|
|Scientific References||PMID: 2953813 by Schmid J., et al. Induction of mRNA for a serine protease and a beta-thromboglobulin- like protein in mitogen-stimulated human leukocytes.|
PMID: 3258865 by Caputo A., et al. Structure and differential mechanisms of regulation of expression of a serine esterase gene in activated human T lymphocytes.
Our Boster Quality Guarantee for Anti-Granzyme B/GZMB Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: SMMC Cell Lysate
Lane 2: HEPA Cell Lysate
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (PA1738)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.