Anti-HAND1 Antibody

Product Name

Anti-HAND1 Antibody

View all HAND1 Antibodies

SKU/Catalog Number

A06496-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-HAND1 Antibody catalog # A06496-2. Tested in WB applications. This antibody reacts with Human,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HAND1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06496-2)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human HAND1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06496-2 is reactive to HAND1 in Human, Rat

Applications

A06496-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

23.627kDa

Background of HAND1

Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) By similarity.

Knoefler M., Gene 224:77-86(1998).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-HAND1 Antibody A06496-2

Western blot (WB) analysis of HAND1 polyclonal antibody at 1:500
Line1:Hela whole cell lysate
Line2:H9C2 whole cell lysate
Line3:
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For HAND1 (Source: Uniprot.org, NCBI)

Alternative Names

BHLHA27; bHLHa27heart- and neural crest derivatives-expressed protein 1; Class A basic helix-loop-helix protein 27; eHand; Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1; HAND1; heart and neural crest derivatives expressed 1; Hxt; Thing1 HAND1 Hxt, Thing1, bHLHa27, eHand heart and neural crest derivatives expressed 1 heart- and neural crest derivatives-expressed protein 1|class A basic helix-loop-helix protein 27|extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1

For more info on HAND1, check out the HAND1 Infographic

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In this infographic, you will see the following information for HAND1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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