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Product Info Summary
| SKU: | A00417-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ID2 Antibody Picoband®
SKU/Catalog Number
A00417-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ID2 Antibody Picoband® catalog # A00417-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ID2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00417-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ID2 recombinant protein (Position: M1-C133).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00417-3 is reactive to ID2 in Human, Mouse, Rat
Observed Molecular Weight
18 kDa
Calculated molecular weight
14.9 kDa
Background of ID2
DNA-binding protein inhibitor ID-2 is a protein that in humans is encoded by the ID2 gene. The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00417-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HEK293 whole cell, human HepG2 whole cell, human Caco-2 whole cell, human U87 whole cell, human PC-3 whole cell, human HL-60 whole cell, human Hela whole cell, rat liver tissue, rat ovary tissue, mouse liver tissue, mouse lung tissue, mouse spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of ID2 using anti-ID2 antibody (A00417-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human HL-60 whole cell lysates,
Lane 7: human Hela whole cell lysates,
Lane 8: rat liver tissue lysates,
Lane 9: rat ovary tissue lysates,
Lane 10: mouse liver tissue lysates,
Lane 11: mouse lung tissue lysates,
Lane 12: mouse spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ID2 antigen affinity purified polyclonal antibody (Catalog # A00417-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ID2 at approximately 18KD. The expected band size for ID2 is at 18KD.
Specific Publications For Anti-ID2 Antibody Picoband® (A00417-3)
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