Anti-HDAC-5 (internal) Antibody

Product Name

Anti-HDAC-5 (internal) Antibody

View all HDAC5 Antibodies

SKU/Catalog Number

A01230-2

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-HDAC-5 (internal) Antibody (Catalog # A01230-2). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-HDAC-5 (internal) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01230-2)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 325-375aa of human HDAC5 protein.

Reactive Species

A01230-2 is reactive to HDAC5 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

122.0 kDa

Background of HDAC5

HDAC5 is a ubiquitous protein responsible for the deacetylation of lysine residues on the N-terminal part of the core histones. A tag for epigenetic repression, Anti-HDAC5 is an enzyme involved in transcriptional regulation, cell cycle progression and developmental events. HDAC5 is a class II histone deacetylase as defined by its similarities to other members of this class. HDAC5 is a major player in muscle differentiation, acting by repressing transcription of myocyte enhancer MEF2C. During differentiation it moves from the nucleus into the cytoplasm, allowing for myocyte enhancer factor expression. Anti-HDAC5 antibody is ideal for researchers interested in Stem Cell, Cardiovascular, or Epigenetics and Nuclear Signaling research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01230-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1: 10,000
IHC: 5 µg/mL
WB: 1ug/ml
Anti-HDAC5 antibody tested in ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~122kDa corresponding to the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information HDAC5

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