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Product Info Summary
| SKU: | PA1597 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-HDJ2/DNAJA1 Antibody Picoband®
SKU/Catalog Number
PA1597
BA3658-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HDJ2/DNAJA1 Antibody catalog # PA1597. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDJ2/DNAJA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1597)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HDJ2, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1597 is reactive to DNAJA1 in Human, Mouse, Rat
Observed Molecular Weight
45 kDa
Calculated molecular weight
44.9 kDa
Background of DNAJA1
DNAJA1 (DnaJ homolog subfamily A member 1), also called DJA1, HSDJ, HSPF4, is a protein that in humans is encoded by the DNAJA1 gene. The deduced 397-amino acid DNAJA1 protein is 32% identical to E. coli DnaJ, with the highest identity in the N-terminal region. Among the known DNAJ homologs in S. cerevisiae, DNAJA1 is most identical to YDJ1, which may be involved in the transport of certain proteins into the mitochondria and endoplasmic reticulum. Dja1 is expressed in mouse testis and prostate by immunohistochemical analysis and Western blot analysis. Transplantation experiments with fluorescence-tagged spermatogonia into Dja1 -/- mice revealed a primary defect of Sertoli cells in maintaining spermiogenesis at steps 8 and 9.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1597 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Positive Control
WB: Rat Brain Tissue, Rat Lung Tissue, Mouse Brain Tissue, Mouse Lung Tissue, U87 Whole Cell, A549 Whole Cell, COLO320 Whole Cell, A431 Whole Cell, HT1080 Whole Cell
ICC/IF: A431 cell
Validation Images & Assay Conditions
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Anti-HDJ2 antibody, PA1597, Western blotting
All lanes: Anti HDJ2 (PA1597) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Lane 4: Mouse Lung Tissue Lysate at 50ug
Lane 5: U87 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: A431 Whole Cell Lysate at 40ug
Lane 9: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
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IF analysis of DNAJA1 using anti-DNAJA1 antibody (PA1597).
DNAJA1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-DNAJA1 Antibody (PA1597) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-HDJ2/DNAJA1 Antibody Picoband® (PA1597)
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1 Customer Q&As for Anti-HDJ2/DNAJA1 Antibody Picoband®
Question
We are currently using anti-HDJ2/DNAJA1 antibody PA1597 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
The anti-HDJ2/DNAJA1 antibody (PA1597) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-17
