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Product Info Summary
| SKU: | A05228-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Helios IKZF2 Antibody
SKU/Catalog Number
A05228-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Helios IKZF2 Antibody catalog # A05228-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Helios IKZF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05228-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human IKZF2. AA range:331-380
Reactive Species
A05228-1 is reactive to IKZF2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
59.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05228-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western blot analysis of Hela cells using Helios Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
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Western blot analysis of the lysates from HeLa cells using IKZF2 antibody.
Specific Publications For Anti-Helios IKZF2 Antibody (A05228-1)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Helios IKZF2 Antibody
Question
Is a blocking peptide available for product anti-Helios antibody (A05228-1)?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
We do provide the blocking peptide for product anti-Helios antibody (A05228-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-26
Question
We are currently using anti-Helios antibody A05228-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The anti-Helios antibody (A05228-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-13
Question
I was wanting to use your anti-Helios antibody for WB for mouse leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
As indicated on the product datasheet, A05228-1 anti-Helios antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-28
Question
See below the WB image, lot number and protocol we used for leukemic t-cell using anti-Helios antibody A05228-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-31
Question
Does A05228-1 anti-Helios antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Singh
Verified customer
Asked: 2013-12-04
Answer
It shows on the product datasheet, A05228-1 anti-Helios antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-12-04
