Anti-HER2 Rabbit Monoclonal Antibody, Clone#RM228

Boster Bio Anti-HER2 Rabbit Monoclonal Antibody, Clone#RM228 catalog # M00010-2. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: M00010-2
Size: 100ul
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-HER2 Rabbit Monoclonal Antibody, Clone#RM228

See all ErbB2/Her2 products

SKU/Catalog Number

M00010-2

Size

100ul

Description

Boster Bio Anti-HER2 Rabbit Monoclonal Antibody, Clone#RM228 catalog # M00010-2. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HER2 Rabbit Monoclonal Antibody, Clone#RM228 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00010-2)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM228

Immunogen

A peptide corresponding to the C-terminus of Human c-erbB-2/HER-2

Reactive Species

M00010-2 is reactive to ERBB2 in Human

Applications

M00010-2 is guaranteed for IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:100 - 1:400 dilution;
Western Blot (WB): 1:1000 dilution.

Validation Images & Assay Conditions

Gene/Protein Information For ERBB2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04626

Gene ID

2064

Gene Name

ERBB2

Full Name

Receptor tyrosine-protein kinase erbB-2

Weight

137.91kDa

Superfamily

protein kinase superfamily

Alternative Names

CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomade ERBB2 CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1 erb-b2 receptor tyrosine kinase 2 receptor tyrosine-protein kinase erbB-2|c-erb B2/neu protein|herstatin|human epidermal growth factor receptor 2|metastatic lymph node gene 19 protein|neuro/glioblastoma derived oncogene homolog|neuroblastoma/glioblastoma derived oncogene homolog|proto-oncogene Neu|proto-oncogene c-ErbB-2|tyrosine kinase-type cell surface receptor HER2|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2|v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2|v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ERBB2, check out the ERBB2 Infographic

ERBB2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ERBB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00010-2 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lapatinib-incorporated lipoprotein-like nanoparticles: preparation and a proposed breast cancer-targeting mechanism

Tong ZJ, Shi NY, Zhang ZJ, Yuan XD, Hong XM. Biosci Rep. 2017 Aug 2;37(4). pii: BSR20170121. doi: 10.1042/BSR20170121. Print 2017 Aug 31. Expression and prognostic value of HER-2/neu in primary breast cancer with sentinel lymph node metastasis

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16 Customer Q&As for Anti-HER2 Rabbit Monoclonal Antibody, Clone#RM228

Question

We ordered your anti-HER2 Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

I took a look at the website and datasheets of our anti-HER2 Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2020-03-31

Question

Can you help my question with product M00010-2, anti-HER2 Rabbit Monoclonal antibody, Clone#RM228. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-23

Question

Is this M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 reactive to the isotypes of ERBB2?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

The immunogen of M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 is A peptide corresponding to the C-terminus of Human c-erbB-2/HER-2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-24

Question

Our lab want to know about using your anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 for positive regulation of transcription by rna polymerase i studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

Thank you for your inquiry. This M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 is validated on breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-10

Question

I see that the anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-28

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-28

Question

Will M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

You can see on the product datasheet, M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-16

Question

Is there a BSA free version of anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 available?

Verified Customer

Verified customer

Asked: 2019-01-30

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-01-30

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-11-16

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-16

Question

Here is the WB image, lot number and protocol we used for fetal brain using anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-09-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-09-19

Question

Is a blocking peptide available for product anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 (M00010-2)?

Verified Customer

Verified customer

Asked: 2017-12-07

Answer

We do provide the blocking peptide for product anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 (M00010-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-12-07

Question

I was wanting to use your anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 for IHC for human fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal brain identification?

G. Moore

Verified customer

Asked: 2017-08-15

Answer

As indicated on the product datasheet, M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-15

Question

Would anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 work for IHC with fetal brain?

K. Parker

Verified customer

Asked: 2017-08-10

Answer

According to the expression profile of fetal brain, ERBB2 is highly expressed in fetal brain. So, it is likely that anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 will work for IHC with fetal brain.

Boster Scientific Support

Answered: 2017-08-10

Question

Our lab were content with the WB result of your anti-HER2 Rabbit Monoclonal antibody, Clone

J. Johnson

Verified customer

Asked: 2016-07-05

Answer

Based on literature, mammary carcinoma does express ERBB2. Generally ERBB2 expresses in isoform 1: cell membrane, isoform 2: cytoplasm. nucleus., isoform 3: cytoplasm. nucleus. Regarding which tissues have ERBB2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 24722188
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 2992089

Boster Scientific Support

Answered: 2016-07-05

Question

We have been able to see staining in human cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 supposed to stain cervix carcinoma erythroleukemia positively?

R. Carter

Verified customer

Asked: 2015-10-28

Answer

From literature cervix carcinoma erythroleukemia does express ERBB2. From Uniprot.org, ERBB2 is expressed in esophagus mucosa, brain, fetal brain, mammary carcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ERBB2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 24722188
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 2992089

Boster Scientific Support

Answered: 2015-10-28

Question

We are currently using anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?

C. Collins

Verified customer

Asked: 2015-10-06

Answer

The anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 (M00010-2) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-10-06

Question

you antibody to test anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 on human fetal brain for research purposes, then I may be interested in using anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Miller

Verified customer

Asked: 2013-09-09

Answer

The products we sell, including anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-09-09

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M00010-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$382.00

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