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Product Info Summary
| SKU: | M00010-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HER2 Rabbit Monoclonal Antibody, Clone#RM228
SKU/Catalog Number
M00010-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HER2 Rabbit Monoclonal Antibody, Clone#RM228 (Catalog # M00010-2). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HER2 Rabbit Monoclonal Antibody, Clone#RM228 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00010-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM228
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of Human c-erbB-2/HER-2
Cross-reactivity
This antibody reacts to Human c-erbB-2/HER-2. This antibody may also react to mouse or rat c-erbB-2/HER-2, as predicted by immunogen homology.
Reactive Species
M00010-2 is reactive to ERBB2 in Human
Calculated molecular weight
137.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00010-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100 dilution
WB: 1:1000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of MCF-7, PC3, Jurkat, and A375 cell lysates using RM228, showed a band of Her2 expressed only in MCF-7 cells.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections.
Specific Publications For Anti-HER2 Rabbit Monoclonal Antibody, Clone#RM228 (M00010-2)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-HER2 Rabbit Monoclonal Antibody, Clone#RM228
Question
We ordered your anti-HER2 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
I took a look at the website and datasheets of our anti-HER2 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2020-03-31
Question
Can you help my question with product M00010-2, anti-HER2 Rabbit Monoclonal antibody, Clone#RM228. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-23
Question
Is this M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 reactive to the isotypes of ERBB2?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The immunogen of M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 is A peptide corresponding to the C-terminus of Human c-erbB-2/HER-2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-24
Question
Our lab want to know about using your anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 for positive regulation of transcription by rna polymerase i studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Thank you for your inquiry. This M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 is validated on breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-10
Question
I see that the anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-28
Question
Will M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
You can see on the product datasheet, M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-16
Question
Is there a BSA free version of anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 available?
Verified Customer
Verified customer
Asked: 2019-01-30
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-16
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-16
Question
Here is the WB image, lot number and protocol we used for fetal brain using anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-19
Question
Is a blocking peptide available for product anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 (M00010-2)?
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
We do provide the blocking peptide for product anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 (M00010-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-12-07
Question
I was wanting to use your anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 for IHC for human fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal brain identification?
G. Moore
Verified customer
Asked: 2017-08-15
Answer
As indicated on the product datasheet, M00010-2 anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-15
Question
Would anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 work for IHC with fetal brain?
K. Parker
Verified customer
Asked: 2017-08-10
Answer
According to the expression profile of fetal brain, ERBB2 is highly expressed in fetal brain. So, it is likely that anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 will work for IHC with fetal brain.
Boster Scientific Support
Answered: 2017-08-10
Question
Our lab were content with the WB result of your anti-HER2 Rabbit Monoclonal antibody, Clone
J. Johnson
Verified customer
Asked: 2016-07-05
Answer
Based on literature, mammary carcinoma does express ERBB2. Generally ERBB2 expresses in isoform 1: cell membrane, isoform 2: cytoplasm. nucleus., isoform 3: cytoplasm. nucleus. Regarding which tissues have ERBB2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 24722188
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 2992089
Boster Scientific Support
Answered: 2016-07-05
Question
We have been able to see staining in human cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 supposed to stain cervix carcinoma erythroleukemia positively?
R. Carter
Verified customer
Asked: 2015-10-28
Answer
From literature cervix carcinoma erythroleukemia does express ERBB2. From Uniprot.org, ERBB2 is expressed in esophagus mucosa, brain, fetal brain, mammary carcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ERBB2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 24722188
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 2992089
Boster Scientific Support
Answered: 2015-10-28
Question
We are currently using anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
C. Collins
Verified customer
Asked: 2015-10-06
Answer
The anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 (M00010-2) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-10-06
Question
you antibody to test anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 on human fetal brain for research purposes, then I may be interested in using anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Miller
Verified customer
Asked: 2013-09-09
Answer
The products we sell, including anti-HER2 Rabbit Monoclonal antibody, Clone#RM228 M00010-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-09-09
