Anti-HFE Antibody Picoband™

Boster Bio Anti-HFE Antibody Picoband™ catalog # A00506-1. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A00506-1
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-HFE Antibody Picoband™

See all HFE products

SKU/Catalog Number

A00506-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HFE Antibody Picoband™ catalog # A00506-1. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HFE Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00506-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human HFE recombinant protein (Position: Q82-R199). Human HFE shares 72.2% and 74.6% amino acid (aa) sequence identity with mouse and rat HFE, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00506-1 is reactive to HFE in Human

Applications

A00506-1 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For HFE (Source: Uniprot.Org, NCBI)

Uniprot ID

Q30201

Gene ID

3077

Gene Name

HFE

Full Name

Hereditary hemochromatosis protein

Weight

40.108kDa

Superfamily

MHC class I family

Alternative Names

dJ221C16.10.1; hemochromatosis; hereditary hemochromatosis protein HLA-H; hereditary hemochromatosis protein; HH; high Fe; HLAH; HLA-HHFE1; MGC103790; MHC class I-like protein HFE; MVCD7 HFE HFE1, HH, HLA-H, MVCD7, TFQTL2 homeostatic iron regulator hereditary hemochromatosis protein|MHC class I-like protein HFE|hereditary hemochromatosis protein HLA-H|high Fe

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HFE, check out the HFE Infographic

HFE infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HFE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A00506-1

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4 Customer Q&As for Anti-HFE Antibody Picoband™

Question

Is there a BSA free version of anti-HFE antibody A00506-1 available?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HFE antibody A00506-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-17

Question

I have a question about product A00506-1, anti-HFE antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-02-15

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00506-1 anti-HFE antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-02-15

Question

Is a blocking peptide available for product anti-HFE antibody (A00506-1)?

Verified Customer

Verified customer

Asked: 2017-11-15

Answer

We do provide the blocking peptide for product anti-HFE antibody (A00506-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-11-15

Question

We are currently using anti-HFE antibody A00506-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?

W. Patel

Verified customer

Asked: 2015-08-25

Answer

The anti-HFE antibody (A00506-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-08-25

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
A00506-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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