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FMO4 antibody (PA1883) performs well in IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of FMO4 using anti-FMO4 antibody (PA1883).
FMO4 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FMO4 Antibody (PA1883) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
FMO4 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FMO4 Antibody (PA1883) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Flavin containing monooxygenase 4/FMO4 Antibody Picoband®
FMO4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, ICC, WB |
Tested Applications
FMO4 antibody was positively detected by WB in:
human THP-1 whole cell, human U251 whole cell, human Jurkat whole cell, human MCF-7 whole cell, rat liver tissue, mouse kidney tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FMO4 antibody was positively detected by ICC/IF in:
MCF-7 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Flavin containing monooxygenase 4/FMO4 Antibody Picoband®
View all FMO4 Antibodies
View all FMO4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Flavin containing monooxygenase 4, different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
63 kDa
Calculated Molecular Weight
63.3 kDa
Background of FMO4
FMO4 (Flavin-containing monooxygenase 4) also known as FMO2, FORMERLY or FMO, ADULT LIVER FORM, is an enzyme that in humans is encoded by the FMO4 gene. By PCR analysis, Dolphin et al. (1992) mapped the FMO4 gene to chromosome 1. Southern blot hybridization with single exon probes demonstrated that human FMO4 and FMO1 are, in each case, the product of a single gene. Lawton et al. (1994) proposed a reclassification of mammalian FMOs. Under this system, FMO2 described by Dolphin et al. (1992) is now designated FMO4.
Cite This Product
Anti-Flavin containing monooxygenase 4/FMO4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1883)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
94%
High Homology
Rat
89%
Moderate Homology
Cow
89%
Moderate Homology
Mouse
83%
Moderate Homology
Rabbit
78%
Moderate Homology
Sheep
78%
Moderate Homology
Dog
78%
Moderate Homology
Zebrafish
28%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FMO4 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for FMO4 antibody
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Product Promise
We promise Anti-Flavin containing monooxygenase 4/FMO4 Antibody Picoband® performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1883
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026