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- Table of Contents
HIF1AN antibody (M02116-1) performs well in IF, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling HIF1AN with M02116-1 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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Anti-HIF1AN Antibody (C-term)
HIF1AN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | IF, WB |
Recommend Dilution
Application
Dilution
Species
IF: 1:25
WB: 1:4000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Product Information
Product Name
Anti-HIF1AN Antibody (C-term)
View all HIF1AN Antibodies
View all HIF1AN Antibodies
Uniprot
Gene ID
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
931CT3.1.1
Isotype
IgG1,k
Immunogen
This HIF1AN antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-349 amino acids from human HIF1AN.
Calculated Molecular Weight
40.3 kDa
Background of HIF1AN
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Cite This Product
Anti-HIF1AN Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02116-1)
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Product Promise
We promise Anti-HIF1AN Antibody (C-term) performs excellently in relevant applications for IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M02116-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026