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Product Info Summary
| SKU: | M02116-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-HIF1AN Antibody (C-term)
SKU/Catalog Number
M02116-1
Size
50 µl
Description
Boster Bio Anti-HIF1AN Antibody (C-term) (Catalog # M02116-1). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HIF1AN Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02116-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
931CT3.1.1
Isotype
IgG1,k
Immunogen
This HIF1AN antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-349 amino acids from human HIF1AN.
Reactive Species
M02116-1 is reactive to HIF1AN in Human
Calculated molecular weight
40.3 kDa
Background of HIF1AN
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02116-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:4000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling HIF1AN with M02116-1 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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All lanes : Anti-HIF1AN Antibody (C-term) at 1:4000 dilution
Lane 1: 293 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: ZR-75-1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 40 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-HIF1AN Antibody (C-term) (M02116-1)
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