Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody

Boster Bio Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody catalog # M00415. Tested in IP, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M00415
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IP, WB

Product Name

Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody

See all EGLN1/PHD2 products

SKU/Catalog Number

M00415

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody catalog # M00415. Tested in IP, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00415)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACEF-5

Immunogen

A synthesized peptide derived from human PHD2 / prolyl hydroxylase Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

Reactive Species

M00415 is reactive to EGLN1 in Human

Applications

M00415 is guaranteed for IP, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500~1:2000 IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For EGLN1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9GZT9

Gene ID

54583

Gene Name

EGLN1

Full Name

Egl nine homolog 1

Weight

46.021kDa

Alternative Names

C1orf12; C1orf12HPH2; EC 1.14.11; EC 1.14.11.-; ECYT3; egl nine homolog 1 (C. elegans); egl nine homolog 1; egl nine-like protein 1; EGLN1; HIF prolyl hydroxylase 2; HIFPH2 EGL nine (C.elegans) homolog 1; HIFPH2; HIF-PH2; HIF-prolyl hydroxylase 2; HPH2; HPH-2; Hypoxia-inducible factor prolyl hydroxylase 2; PHD2; PHD2SM20; Prolyl hydroxylase domain-containing protein 2; SM20; SM-20; zinc finger MYND domain-containing protein 6; ZMYND6; ZMYND6DKFZp761F179 EGLN1 C1orf12, ECYT3, HALAH, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6 egl-9 family hypoxia inducible factor 1 egl nine homolog 1|HIF-prolyl hydroxylase 2|egl nine-like protein 1|hypoxia-inducible factor prolyl hydroxylase 2|prolyl hydroxylase domain-containing protein 2|zinc finger MYND domain-containing protein 6

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EGLN1, check out the EGLN1 Infographic

EGLN1 infographic

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In this infographic you will see the following information for EGLN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody

Question

Does anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 work on goat WB with aorta?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

Our lab technicians have not tested anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 on goat. You can run a BLAST between goat and the immunogen sequence of anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat aorta in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-12

Question

We are currently using anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-10-26

Answer

The anti-PHD2 / prolyl hydroxylase Monoclonal antibody (M00415) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-26

Question

My question regards to test anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 on human promyelocytic leukemia for research purposes, then I may be interested in using anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-08-22

Answer

The products we sell, including anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-08-22

Question

Will anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 work for IP with promyelocytic leukemia?

J. Williams

Verified customer

Asked: 2014-11-10

Answer

According to the expression profile of promyelocytic leukemia, EGLN1 is highly expressed in promyelocytic leukemia. So, it is likely that anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 will work for IP with promyelocytic leukemia.

Boster Scientific Support

Answered: 2014-11-10

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M00415
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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