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Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody
Boster Bio Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody catalog # M00415. Tested in IP, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00415)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human PHD2 / prolyl hydroxylase Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
M00415 is reactive to EGLN1 in Human
M00415 is guaranteed for IP, WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB 1:500~1:2000 IP 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EGLN1 expression in SH-SY5Y cell lysate using anti-EGLN1 antibody (M00415).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGLN1 antigen affinity purified polyclonal antibody (Catalog # M00415) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for EGLN1.
Gene/Protein Information For EGLN1 (Source: Uniprot.Org, NCBI)
Egl nine homolog 1
C1orf12; C1orf12HPH2; EC 1.14.11; EC 1.14.11.-; ECYT3; egl nine homolog 1 (C. elegans); egl nine homolog 1; egl nine-like protein 1; EGLN1; HIF prolyl hydroxylase 2; HIFPH2 EGL nine (C.elegans) homolog 1; HIFPH2; HIF-PH2; HIF-prolyl hydroxylase 2; HPH2; HPH-2; Hypoxia-inducible factor prolyl hydroxylase 2; PHD2; PHD2SM20; Prolyl hydroxylase domain-containing protein 2; SM20; SM-20; zinc finger MYND domain-containing protein 6; ZMYND6; ZMYND6DKFZp761F179 EGLN1 C1orf12, ECYT3, HALAH, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6 egl-9 family hypoxia inducible factor 1 egl nine homolog 1|HIF-prolyl hydroxylase 2|egl nine-like protein 1|hypoxia-inducible factor prolyl hydroxylase 2|prolyl hydroxylase domain-containing protein 2|zinc finger MYND domain-containing protein 6*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on EGLN1, check out the EGLN1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EGLN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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