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Product Info Summary
| SKU: | M00415 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody
SKU/Catalog Number
M00415
BM5273 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody catalog # M00415. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00415)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACEF-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PHD2 / prolyl hydroxylase Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
Reactive Species
M00415 is reactive to EGLN1 in Human
Observed Molecular Weight
50 kDa
Calculated molecular weight
46.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00415 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of PHD2 / prolyl hydroxylase expression in SH-SY5Y cell lysate.
Specific Publications For Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody (M00415)
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Customer Q&As
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4 Customer Q&As for Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody
Question
Does anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 work on goat WB with aorta?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Our lab technicians have not tested anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 on goat. You can run a BLAST between goat and the immunogen sequence of anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat aorta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
We are currently using anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
The anti-PHD2 / prolyl hydroxylase Monoclonal antibody (M00415) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-26
Question
My question regards to test anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 on human promyelocytic leukemia for research purposes, then I may be interested in using anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
The products we sell, including anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-22
Question
Will anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 work for IP with promyelocytic leukemia?
J. Williams
Verified customer
Asked: 2014-11-10
Answer
According to the expression profile of promyelocytic leukemia, EGLN1 is highly expressed in promyelocytic leukemia. So, it is likely that anti-PHD2 / prolyl hydroxylase Monoclonal antibody M00415 will work for IP with promyelocytic leukemia.
Boster Scientific Support
Answered: 2014-11-10
