Product Info Summary
SKU: | A12477S10-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H3 (Phospho-S10) HIST1H3A Antibody
SKU/Catalog Number
A12477S10-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Histone H3 (Phospho-S10) HIST1H3A Antibody catalog # A12477S10-1. Tested in WB,ICC/IF,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H3 (Phospho-S10) HIST1H3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12477S10-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human FA58A protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12477S10-1 is reactive to H3C1 in Human, Mouse, Rat
Applications
A12477S10-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
15.404kDa
Background of H3C1
Required for normal Golgi ribbon organization and ER-to-Golgi trafficking.
Ross M.T., Nature 434:325-337(2005).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of HIST1H3A using Anti-Histone H3 (Phospho-S10) HIST1H3A Antibody (A12477S10-1).
ICC staining Phospho-Histone H3(S10) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Histone H3 (Phospho-S10) HIST1H3A Antibody A12477S10-1
Western blot analysis of Phospho-Histone H3(S10) on Hela cell lysates using anti-Phospho-Histone H3(S10) antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For H3C1 (Source: Uniprot.org, NCBI)
Gene Name
H3C1
Full Name
Histone H3.1
Weight
15.404kDa
Superfamily
histone H3 family
Alternative Names
Histone H3.1 H3C1 H3/A0, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, H3C1 H3 clustered histone 1 histone H3.1|H3 histone family, member A|histone 1, H3a|histone H3/a|histone cluster 1 H3 family member a|histone cluster 1, H3a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H3C1, check out the H3C1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H3C1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H3 (Phospho-S10) HIST1H3A Antibody (A12477S10-1)
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