Product Info Summary
SKU: | PA1481 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-AIMP2/p38 Antibody
SKU/Catalog Number
PA1481
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AIMP2/p38 Antibody catalog # PA1481. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AIMP2/p38 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1481)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human AIMP2/p38, different from the related rat and mouse sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1481 is reactive to AIMP2 in Human, Mouse, Rat
Applications
PA1481 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
35.349kDa
Background of AIMP2
AIMP2, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 2, also known as AIMP2, is an enzyme that in humans is encoded by the AIMP2 gene. AIMP2 encodes a predicted 312-amino acid protein. The AIMP2 gene is located on chromosome 7p22 flanked by two genes, HRI and PMS2. AIMP2 and HRI overlap slightly and are arranged in a tail-to-tail fashion. AIMP2 and PMS2 are separated by approximately 200 base pairs and are arranged head-to-head. AIMP2 is transcribed in the opposite direction compared to HRI and PMS2. AIMP2 is a scaffold required for the assembly and stability of the multi-tRNA synthetase complex. AIMP2 can work as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AIMP2 using anti-AIMP2 antibody (PA1481).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: Jurkat cell lysates,
Lane 6: CEM cell lysates,
Lane 7: HUT cell lysates,
Lane 8: U93T cell lysates,
Lane 9: U93T cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIMP2 antigen affinity purified polyclonal antibody (Catalog # PA1481) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Click image to see more details
Figure 2. IHC analysis of AIMP2 using anti-AIMP2 antibody (PA1481).
AIMP2 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AIMP2 Antibody (PA1481) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of AIMP2 using anti-AIMP2 antibody (PA1481).
AIMP2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AIMP2 Antibody (PA1481) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For AIMP2 (Source: Uniprot.org, NCBI)
Gene Name
AIMP2
Full Name
Aminoacyl tRNA synthase complex-interacting multifunctional protein 2
Weight
35.349kDa
Alternative Names
aminoacyl tRNA synthase complex-interacting multifunctional protein 2; aminoacyl tRNA synthetase complex-interacting multifunctional protein 2; ARS-interacting multi-functional protein 2; JTV1JTV-1; Multisynthase complex auxiliary component p38; multisynthetase complex auxiliary component p38; P38; PRO0992; Protein JTV-1 AIMP2 HLD17, JTV-1, JTV1, P38 aminoacyl tRNA synthetase complex interacting multifunctional protein 2 aminoacyl tRNA synthase complex-interacting multifunctional protein 2|ARS-interacting multi-functional protein 2|multisynthase complex auxiliary component p38|multisynthetase complex auxiliary component p38|protein JTV-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AIMP2, check out the AIMP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AIMP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AIMP2/p38 Antibody (PA1481)
Hello CJ!
PA1481 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu T,Liu Q,Li Y,Li H,Chen L,Yang X,Tang Q,Pu S,Kuang J,Li R,Huang Y,Zhang J,Zhang Z,Zhou J,Huang C,Zhang G,Zhao Y,Zou M,Jiang W,Mo L,He J.Feeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway.J Exp Med.2021 Jun 7;218(6):e20201203.doi:10. 1084/jem. 20201203.PMID:33856409.
Species: Mouse
PA1481 usage in article: APP:WB, SAMPLE:ADIPOCYTES, DILUTION:1:2000
Ma G,Kimatu BM,Yang W,Pei F,Zhao L,Du H,Su A,Hu Q,Xiao H.Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential.J Food Sci.2020 Sep;85(9):2822-2831. doi:10.1111/1750-3841.15375.Epub 2020 Aug 14
Species: Mouse
PA1481 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA
Ji F,Wang Y,Yuan J,Wu Q,Wang J,Liu D.The potential role of stromal cell-derived factor-1α/CXCR4/CXCR7 axis in adipose-derived mesenchymal stem cells.J Cell Physiol.2020 Apr;235(4):3548-3557.doi:10.1002/jcp.29243.Epub 2019 Sep 30.PMID:31566725.
Species: Rat
PA1481 usage in article: APP:IF, SAMPLE:ADSCS, DILUTION:1:400
Zheng Q, Liu Y, Liu W, Ma F, Zhou Y, Chen M, Chang J, Wang Y, Yang G, He G. Mol Med Rep. 2014 Jul;10(1):89-94. Doi: 10.3892/Mmr.2014.2175. Epub 2014 Apr 24. Cucurbitacin B Inhibits Growth And Induces Apoptosis Through The Jak2/Stat3 And Mapk Pathw...
Zhao B, Li X. Oncol Rep. 2014 Jun;31(6):2769-75. Doi: 10.3892/Or.2014.3126. Epub 2014 Apr 3. Altholactone Induces Reactive Oxygen Species-Mediated Apoptosis In Bladder Cancer T24 Cells Through Mitochondrial Dysfunction, Mapk-P38 Activation And Akt...
Sun Y, Yang J, Wang Lz, Sun Lr, Dong Q. Hum Exp Toxicol. 2014 Aug;33(8):855-62. Doi: 10.1177/0960327113511475. Epub 2013 Nov 25. Crocin Attenuates Cisplatin-Induced Liver Injury In The Mice.
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Customer Q&As
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1 Customer Q&As for Anti-AIMP2/p38 Antibody
Question
We are currently using anti-AIMP2/p38 antibody PA1481 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The anti-AIMP2/p38 antibody (PA1481) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-25