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Product Info Summary
| SKU: | PA1481 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-AIMP2/p38 Antibody Picoband®
SKU/Catalog Number
PA1481
BA3623 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AIMP2/p38 Antibody catalog # PA1481. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AIMP2/p38 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1481)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human AIMP2/p38, different from the related rat and mouse sequences by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1481 is reactive to AIMP2 in Human, Mouse, Rat
Observed Molecular Weight
36 kDa
Calculated molecular weight
35.3 kDa
Background of AIMP2
AIMP2, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 2, also known as AIMP2, is an enzyme that in humans is encoded by the AIMP2 gene. AIMP2 encodes a predicted 312-amino acid protein. The AIMP2 gene is located on chromosome 7p22 flanked by two genes, HRI and PMS2. AIMP2 and HRI overlap slightly and are arranged in a tail-to-tail fashion. AIMP2 and PMS2 are separated by approximately 200 base pairs and are arranged head-to-head. AIMP2 is transcribed in the opposite direction compared to HRI and PMS2. AIMP2 is a scaffold required for the assembly and stability of the multi-tRNA synthetase complex. AIMP2 can work as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1481 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: human MCF-7 whole cell, human U2OS whole cell, hman A549 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
IHC: human rectal cancer tissue, rat intestine tissue
Validation Images & Assay Conditions
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Western blot analysis of AIMP2/P38 using anti-AIMP2/P38 antibody (PA1481).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: hman A549 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIMP2/P38 antigen affinity purified polyclonal antibody (PA1481) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AIMP2/P38 at approximately 36 kDa. The expected band size for AIMP2/P38 is at 36 kDa.
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IHC analysis of AIMP2 using anti-AIMP2 antibody (PA1481).
AIMP2 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AIMP2 Antibody (PA1481) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of AIMP2 using anti-AIMP2 antibody (PA1481).
AIMP2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AIMP2 Antibody (PA1481) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-AIMP2/p38 Antibody Picoband® (PA1481)
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1 Customer Q&As for Anti-AIMP2/p38 Antibody Picoband®
Question
We are currently using anti-AIMP2/p38 antibody PA1481 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The anti-AIMP2/p38 antibody (PA1481) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-25
